Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I2 R1 14 51.3 1601205 98.8% 1581990 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,386 A→C W4G (TGG→GGG)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,3860AC100.0% 171.2 / NA 43W4G (TGG→GGG) metJtranscriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/24);  total (19/24)

GTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAAT  >  NC_000913/4128238‑4128529
                                                                                                                                                    |                                                                                                                                               
gTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCtt                                                                                                                                               >  1:226516/1‑151 (MQ=255)
   gCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTc                                                                                                                                              >  1:547352/1‑149 (MQ=255)
          gCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATgag                                                                                                                                     <  1:276389/151‑1 (MQ=255)
            aGGTTGTTCACCTGACGATGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGAt                                                                                                                                   <  1:232383/151‑1 (MQ=255)
                 gttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACtt                                                                                                                               <  2:756244/150‑1 (MQ=255)
                    cACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAAt                                                                                                                            <  2:585052/150‑1 (MQ=255)
                      ccTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCct                                                                                                                         <  2:449493/151‑1 (MQ=255)
                            gacgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGt                                                                                                                   >  2:297270/1‑151 (MQ=255)
                            gacgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGt                                                                                                                   >  2:493112/1‑151 (MQ=255)
                             acgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTc                                                                                                                  <  2:492437/151‑1 (MQ=255)
                                  tgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATa                                                                                                              >  2:240111/1‑150 (MQ=255)
                                  tgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATa                                                                                                              >  2:725124/1‑150 (MQ=255)
                                   gcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATaa                                                                                                             <  2:25173/150‑1 (MQ=255)
                                      ttCATCGGGGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTAACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAATTTg                                                                                                         >  1:214065/1‑151 (MQ=255)
                                               gAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagac                                                                                                >  2:354487/1‑151 (MQ=255)
                                                 ggATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAgaccagacca                                                                                              <  2:630940/151‑1 (MQ=255)
                                                     ttttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACcacac                                                                                           <  2:7068/150‑1 (MQ=255)
                                                      tttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACcacaca                                                                                          <  1:338079/150‑1 (MQ=255)
                                                          acacCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGa                                                                                     <  2:750294/151‑1 (MQ=255)
                                                                tAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtgtg                                                                                 <  1:614359/149‑1 (MQ=255)
                                                                          ggAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCg                                                                      <  2:782825/150‑1 (MQ=255)
                                                                             aaCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTaa                                                                   >  1:441506/1‑150 (MQ=255)
                                                                                cGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAAccc                                                               >  2:542182/1‑151 (MQ=255)
                                                                                 gTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCg                                                              >  1:423725/1‑151 (MQ=255)
                                                                                   aaTCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGc                                                             >  1:387374/1‑150 (MQ=255)
                                                                                        ttttGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGt                                                        <  1:493063/150‑1 (MQ=255)
                                                                                            gACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTAc                                                    <  2:242410/150‑1 (MQ=255)
                                                                                             aCTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCg                                                  >  2:552562/1‑151 (MQ=255)
                                                                                                  ttCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTAc                                               >  1:626933/1‑149 (MQ=255)
                                                                                                   tCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAg                                             <  2:538496/150‑1 (MQ=255)
                                                                                                          tcttGCCGTGCTCAGCGTATGGGCTGAGATATTCGCCGCTCCCTTCCGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTAcc                                     >  1:781448/1‑151 (MQ=255)
                                                                                                             tGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGt                                   <  1:297806/150‑1 (MQ=255)
                                                                                                              gCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTg                                  <  2:226551/150‑1 (MQ=255)
                                                                                                              gCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGa                                 <  2:403061/151‑1 (MQ=255)
                                                                                                                      cAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCgtg                          <  1:297235/150‑1 (MQ=255)
                                                                                                                       aGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCgtgt                         >  2:73588/1‑150 (MQ=255)
                                                                                                                          gTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATg                     <  2:375702/151‑1 (MQ=255)
                                                                                                                           tATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGc                    >  1:665564/1‑151 (MQ=255)
                                                                                                                           tATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGc                    >  1:107709/1‑151 (MQ=255)
                                                                                                                             tGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCAc                  >  2:529534/1‑151 (MQ=255)
                                                                                                                                   gATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCg            <  2:404735/151‑1 (MQ=255)
                                                                                                                                           cgccgcTCCCTTCAGCCATGAGATGCTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTtata    <  1:92373/151‑1 (MQ=255)
                                                                                                                                              cgcTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAAt  <  1:6858/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAAT  >  NC_000913/4128238‑4128529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A