Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I3 R1 16 56.1 1756558 98.6% 1731966 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,386 A→C W4G (TGG→GGG)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,3860AC100.0% 188.8 / NA 48W4G (TGG→GGG) metJtranscriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/29);  total (19/29)

AGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCAT  >  NC_000913/4128240‑4128533
                                                                                                                                                  |                                                                                                                                                   
aGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCa                                                                                                                                                 <  1:296217/151‑1 (MQ=255)
 gCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAg                                                                                                                                                <  1:368114/151‑1 (MQ=255)
   gTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGcc                                                                                                                                              >  2:229161/1‑151 (MQ=255)
     gACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCa                                                                                                                                             <  2:642801/150‑1 (MQ=255)
            gttgttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATAc                                                                                                                                     <  1:206871/151‑1 (MQ=255)
                  cACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAAt                                                                                                                                <  2:606159/150‑1 (MQ=255)
                     cTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCctc                                                                                                                            >  1:764889/1‑151 (MQ=255)
                             gcgtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCaa                                                                                                                    <  1:368446/151‑1 (MQ=255)
                               gtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAAt                                                                                                                   >  1:234473/1‑150 (MQ=255)
                               gtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAAt                                                                                                                   >  2:457169/1‑150 (MQ=255)
                                 gcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATaa                                                                                                                 >  1:100824/1‑150 (MQ=255)
                                 gcgtTCATCGGTGAGGATTTTAAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATaaa                                                                                                                <  2:602369/151‑1 (MQ=255)
                                  cgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAt                                                                                                               >  2:599027/1‑151 (MQ=255)
                                   gtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAtt                                                                                                              <  2:252810/151‑1 (MQ=255)
                                      cATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTga                                                                                                            <  2:476442/150‑1 (MQ=255)
                                         cGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacc                                                                                                        >  1:376278/1‑151 (MQ=255)
                                         cGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacc                                                                                                        <  2:656302/151‑1 (MQ=255)
                                             gAGGATTTTTAACCCCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTgagacgagac                                                                                                    <  1:260548/151‑1 (MQ=255)
                                               ggATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAgaccagacca                                                                                                  <  1:795738/151‑1 (MQ=255)
                                                gATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACcac                                                                                                 >  2:662313/1‑151 (MQ=255)
                                                 aTTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACcac                                                                                                 >  1:604469/1‑150 (MQ=255)
                                                    tttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACcacaca                                                                                              <  1:269972/150‑1 (MQ=255)
                                                      tAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGtt                                                                                           >  2:817576/1‑151 (MQ=255)
                                                       aaCACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTg                                                                                          <  2:470861/151‑1 (MQ=255)
                                                           ccTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtg                                                                                       <  1:8341/150‑1 (MQ=255)
                                                                agagGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGt                                                                                  >  2:286113/1‑150 (MQ=255)
                                                                 gagGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTAc                                                                                >  2:143474/1‑151 (MQ=255)
                                                                  agGAATTGAAACCGTAATCTGTTTGACTTGTTCACTCTTCTTGCCGTACTCAGCGTATGGGCTGATATATTCGCCGCTCCCTACAGCCATGAGATACTTAATCCTCGTCGTCAATAAATTGAGACCAGACCACACAGTTTATGTGGGTAcg                                                                               <  1:256502/151‑2 (MQ=255)
                                                                  agGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACt                                                                               <  1:636934/151‑1 (MQ=255)
                                                                      aTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGAcc                                                                           <  2:444230/151‑1 (MQ=255)
                                                                                    cTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCAt                                                               >  1:366303/1‑149 (MQ=255)
                                                                                      ttttGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATa                                                              <  2:382666/148‑1 (MQ=255)
                                                                                        ttGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGttt                                                          >  1:247716/1‑150 (MQ=255)
                                                                                            cTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGt                                                     >  1:254048/1‑151 (MQ=255)
                                                                                                 tCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAgg                                                >  1:647370/1‑151 (MQ=255)
                                                                                                        tcttGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGtt                                            <  1:74133/148‑1 (MQ=255)
                                                                                                           tGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCg                                        <  1:669439/149‑1 (MQ=255)
                                                                                                            gCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTg                                      >  2:854975/1‑150 (MQ=255)
                                                                                                            gCCGTGCTCAGCGTATGGGCGGATATATTCTCCGCTCCCTTCAGCCATGAGATGCTTAATCCTCGTCGTCAATAAATGGAGACCAGGCCACACAGTTGATGTGGGTACTGACCGTAAACCGGCATAGGTGACCGTACAGGCGTTACCGGGa                                     <  1:705711/151‑1 (MQ=255)
                                                                                                                    cAGCGTATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCgt                               <  1:275174/149‑1 (MQ=255)
                                                                                                                                 gATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGt                  <  1:40659/149‑1 (MQ=255)
                                                                                                                                 gATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTc                 <  2:764966/150‑1 (MQ=255)
                                                                                                                                   tatatTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGc              >  1:155309/1‑151 (MQ=255)
                                                                                                                                    atatTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACCCAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGc              <  1:859841/150‑1 (MQ=255)
                                                                                                                                      atTCTCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAGTGCACCTGTCGGCGt            <  1:292565/150‑1 (MQ=255)
                                                                                                                                      atTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGt            <  1:598961/150‑1 (MQ=255)
                                                                                                                                        tCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGa          <  2:273592/150‑1 (MQ=255)
                                                                                                                                               tCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCat  >  1:478572/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCAT  >  NC_000913/4128240‑4128533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A