Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,733,738	T→C	V513A (GTC→GCC)	xylG →	D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,733,738	0	T	C	100.0%	189.6 / NA	52	V513A (GTC→GCC)	xylG	D‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/25); total (27/25)

CCTGAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAGCGCCCGT  >  NC_000913/3733596‑3733884

ccTGAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTg                                                                                                                                            >  2:387908/1‑151 (MQ=255)

  tGAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGa                                                                                                                                           >  1:733605/1‑150 (MQ=255)

   gAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGaa                                                                                                                                          >  2:879319/1‑150 (MQ=255)

    aaGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAAt                                                                                                                                        >  1:880212/1‑151 (MQ=255)

        gCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTgg                                                                                                                                     <  2:226025/150‑1 (MQ=255)

           cGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCg                                                                                                                                  <  1:542203/150‑1 (MQ=255)

            ggCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTa                                                                                                                                <  1:325851/151‑1 (MQ=255)

                ttAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCg                                                                                                                             >  2:613402/1‑150 (MQ=255)

                 tAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCg                                                                                                                             <  1:685467/149‑1 (MQ=255)

                  aGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGAc                                                                                                                           >  1:88675/1‑150 (MQ=255)

                    cGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATc                                                                                                                        <  2:750364/151‑1 (MQ=255)

                    cGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATc                                                                                                                        <  1:349287/151‑1 (MQ=255)

                        cGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGt                                                                                                                    >  2:760366/1‑151 (MQ=255)

                                         tGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGa                                                                                                    >  1:361682/1‑150 (MQ=255)

                                          gAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGaaa                                                                                                  <  2:471027/151‑1 (MQ=255)

                                           aaGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAAt                                                                                                 >  1:397377/1‑151 (MQ=255)

                                            aGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATc                                                                                                <  2:826734/151‑1 (MQ=255)

                                             gggAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATc                                                                                                >  2:535796/1‑150 (MQ=255)

                                                 aaCTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGa                                                                                           <  2:465463/151‑1 (MQ=255)

                                                     aaaaGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAAt                                                                                         <  1:536834/149‑1 (MQ=255)

                                                          ccAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAgg                                                                                  <  1:244072/151‑1 (MQ=255)

                                                              ccTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCtt                                                                              <  2:190374/151‑1 (MQ=255)

                                                                    aaaTCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGtgat                                                                        >  2:718319/1‑151 (MQ=255)

                                                                        cATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGAtt                                                                    <  1:516983/151‑1 (MQ=255)

                                                                         aTAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTg                                                                   >  1:336310/1‑151 (MQ=255)

                                                                                gACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTat                                                            <  1:493172/151‑1 (MQ=255)

                                                                                 aCTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTatc                                                           <  2:127614/151‑1 (MQ=255)

                                                                                     aGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCg                                                       >  2:624609/1‑151 (MQ=255)

                                                                                      ggAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGc                                                      <  2:779944/151‑1 (MQ=255)

                                                                                       gAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCa                                                     <  1:814095/151‑1 (MQ=255)

                                                                                              tGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCAttc                                              >  2:639331/1‑149 (MQ=255)

                                                                                                   gAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTc                                         <  2:218128/151‑1 (MQ=255)

                                                                                                    aaGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCt                                        >  2:572003/1‑151 (MQ=255)

                                                                                                     aGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCtt                                       <  1:129820/151‑1 (MQ=255)

                                                                                                        cGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTAc                                    >  2:279056/1‑151 (MQ=255)

                                                                                                           aTTGAGGAGCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTg                                 <  1:781181/151‑1 (MQ=255)

                                                                                                              gaggagCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGac                              >  2:68782/1‑151 (MQ=255)

                                                                                                               aggagCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGacc                             >  2:41989/1‑151 (MQ=255)

                                                                                                                  agCGAACATCATGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGaccacc                          <  1:78171/151‑1 (MQ=255)

                                                                                                                        catcatGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATgg                     <  2:645937/150‑1 (MQ=255)

                                                                                                                             tGTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCt                >  2:82460/1‑150 (MQ=255)

                                                                                                                              gTCGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCCCCGATGGTGCCTa               <  2:370911/150‑1 (MQ=255)

                                                                                                                                cGAAAAGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACt             <  1:636463/150‑1 (MQ=255)

                                                                                                                                  aaaaGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGACTACTTa           >  2:458526/1‑150 (MQ=255)

                                                                                                                                   aaaGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTa           >  1:279799/1‑149 (MQ=255)

                                                                                                                                    aaGCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAgc        >  1:753937/1‑151 (MQ=255)

                                                                                                                                      gCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAgcgc      >  1:255178/1‑151 (MQ=255)

                                                                                                                                      gCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAgcgc      <  1:718368/151‑1 (MQ=255)

                                                                                                                                      gCAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAgcgc      >  1:566786/1‑151 (MQ=255)

                                                                                                                                       cAATCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAGCGcc     >  1:608344/1‑151 (MQ=255)

                                                                                                                                        aaTCCGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAGCGccc    >  1:164819/1‑151 (MQ=255)

                                                                                                                                           ccGCCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAGCGCCCGt  >  1:73876/1‑150 (MQ=255)

CCTGAAGTGCTCGGCCTTAGCGATCGTGTACTGGTGATGCATGAAGGGAAACTAAAAGCCAACCTGATAAATCATAACCTGACTCAGGAGCAGGTGATGGAAGCCGCATTGAGGAGCGAACATCATGTCGAAAAGCAATCCGTCTGAAGTGAAATTGGCCGTACCGACATCCGGTGGCTTCTCCGGGCTGAAATCACTGAATTTGCAGGTCTTCGTGATGATTGCAGCTATCATCGCAATCATGCTGTTCTTTACCTGGACCACCGATGGTGCCTACTTAAGCGCCCGT  >  NC_000913/3733596‑3733884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A