Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,873,439	G→A	D58D (GAC→GAT)	dgoA ←	2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,873,439	0	G	A	100.0%	201.8 / NA	53	D58D (GAC→GAT)	dgoA	2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (31/22); total (31/22)

AGCCGGGGCAGACGGTCATGCCGTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTGTCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCAAAATGGCGAT  >  NC_000913/3873291‑3873585

aGCCGGGGCAGACGGTCATGCCGTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATc                                                                                                                                                  >  2:370413/1‑151 (MQ=255)

aGCCGGGGCAGACGGTCATGCCGTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATc                                                                                                                                                  >  2:122013/1‑151 (MQ=255)

       gCAGACGGTCATGCCGTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTAc                                                                                                                                           >  2:230705/1‑151 (MQ=255)

         aGACGGTCATGCCGTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACg                                                                                                                                          <  1:613302/150‑1 (MQ=255)

                        aGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCgg                                                                                                                           <  2:592159/150‑1 (MQ=255)

                          cccACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGaa                                                                                                                        <  2:366166/151‑1 (MQ=255)

                                gCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCttt                                                                                                                  <  1:657843/151‑1 (MQ=255)

                                 cACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCttt                                                                                                                  >  1:904551/1‑150 (MQ=255)

                                  aCGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGc                                                                                                                <  2:126164/151‑1 (MQ=255)

                                   cggcggATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCttt                                                                                                                  >  2:80315/1‑148 (MQ=255)

                                     gcggATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTcc                                                                                                             >  2:560862/1‑151 (MQ=255)

                                      cggATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTccc                                                                                                            <  1:564578/151‑1 (MQ=255)

                                      cggATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTccc                                                                                                            >  2:529292/1‑151 (MQ=255)

                                          tcacttcactATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCAtt                                                                                                         >  2:465736/1‑150 (MQ=255)

                                               tcactATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTgggg                                                                                                   >  1:820948/1‑151 (MQ=255)

                                               tcactATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTggg                                                                                                    <  2:365462/150‑1 (MQ=255)

                                                cactATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTgggg                                                                                                   <  2:710724/150‑1 (MQ=255)

                                                        atatTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGGATTCAGCg                                                                                          >  2:472033/1‑151 (MQ=255)

                                                        atatTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTACCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCg                                                                                          >  1:714501/1‑151 (MQ=255)

                                                          atTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCgg                                                                                         <  2:159900/150‑1 (MQ=255)

                                                          atTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCgg                                                                                         <  1:57384/150‑1 (MQ=255)

                                                            tGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGAt                                                                                      >  1:741533/1‑151 (MQ=255)

                                                              ggCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGAttt                                                                                    <  2:778622/151‑1 (MQ=255)

                                                               gCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTc                                                                                   <  2:506685/151‑1 (MQ=255)

                                                                      gATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACcgc                                                                             >  2:751406/1‑150 (MQ=255)

                                                                            cTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAAc                                                                      >  1:152912/1‑151 (MQ=255)

                                                                              gACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCCACCGCGTCGAAccc                                                                    >  2:27119/1‑151 (MQ=255)

                                                                               aCAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAAccc                                                                    >  2:850957/1‑150 (MQ=255)

                                                                               aCAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCg                                                                   >  1:661715/1‑151 (MQ=255)

                                                                                 aGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCgg                                                                  >  2:280297/1‑150 (MQ=255)

                                                                                     cATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACTCATCAACGATGGCGGAAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGt                                                               >  2:607521/1‑149 (MQ=255)

                                                                                        ccTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATc                                                          <  2:319033/151‑1 (MQ=255)

                                                                                          tGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCAc                                                        >  2:231869/1‑151 (MQ=255)

                                                                                            gCGAGCGCATCGGCCTGTTCAGCTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCAGCAACGATGGAGTGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACAGCGTCGCACCCgg                                                                  <  2:662866/139‑1 (MQ=255)

                                                                                              gAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACcgcg                                                    >  1:126327/1‑151 (MQ=255)

                                                                                              gAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACcgcg                                                    <  2:288656/151‑1 (MQ=255)

                                                                                                gcgcATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGGATGCTTTGCTCCCGTTGTGGGGAATTCAGCGGGTTTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGcc                                                  >  1:498127/1‑151 (MQ=255)

                                                                                                      cGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATg                                            >  1:19200/1‑151 (MQ=255)

                                                                                                           tGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGcc                                        >  2:562981/1‑150 (MQ=255)

                                                                                                            gTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGcc                                        >  2:479858/1‑149 (MQ=255)

                                                                                                            gTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGcc                                        <  1:415061/149‑1 (MQ=255)

                                                                                                                      cAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCtcgtcg                            >  2:682738/1‑151 (MQ=255)

                                                                                                                      cAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCtcgtc                             <  1:682341/150‑1 (MQ=255)

                                                                                                                       aGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCgg                           <  1:114476/151‑1 (MQ=255)

                                                                                                                         taccgtaccTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCggg                          >  2:466440/1‑150 (MQ=255)

                                                                                                                          accgtaccTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCg                        <  2:594882/151‑1 (MQ=255)

                                                                                                                                    cgcCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACcgcg              >  1:189835/1‑151 (MQ=255)

                                                                                                                                        aaTCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCaaa          <  2:565399/151‑1 (MQ=255)

                                                                                                                                            aaCGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGGCGGGCGTAATACCGCGCAAAATgg      >  2:168298/1‑151 (MQ=255)

                                                                                                                                            aaCGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCCGCAGCGCCTCGTCGGGCGTAATACCGCGCAAAATgg      >  1:22540/1‑151 (MQ=255)

                                                                                                                                              cGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCAAAATGGCg    >  2:260984/1‑151 (MQ=255)

                                                                                                                                              cGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCAAAATGGCg    <  2:879179/151‑1 (MQ=255)

                                                                                                                                                ccTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCAAAATGGCGAt  <  2:59069/151‑1 (MQ=255)

AGCCGGGGCAGACGGTCATGCCGTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTGTCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCAAAATGGCGAT  >  NC_000913/3873291‑3873585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A