Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,873,439	G→A	D58D (GAC→GAT)	dgoA ←	2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,873,439	0	G	A	100.0%	185.5 / NA	49	D58D (GAC→GAT)	dgoA	2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/34); total (15/34)

AGCCGGGGCAGACGGTCATGCCGTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTGTCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCAAAATGGCGAT  >  NC_000913/3873291‑3873585

aGCCGGGGCAGACGGTCATGCCGTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATc                                                                                                                                                  <  2:276479/151‑1 (MQ=255)

 gCCGGGGCAGACGGTCATGCCGTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCg                                                                                                                                                 <  1:752712/151‑1 (MQ=255)

   cGGGGCAGACGGTCATGCCGTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGcc                                                                                                                                               <  2:412692/151‑1 (MQ=255)

     gggCAGACGGTCATGCCGTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGt                                                                                                                                             <  1:182801/151‑1 (MQ=255)

                   gCCGTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGAt                                                                                                                                <  2:29430/150‑1 (MQ=255)

                      gTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCg                                                                                                                            <  1:343231/151‑1 (MQ=255)

                      gTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCg                                                                                                                            <  2:352472/151‑1 (MQ=255)

                           ccACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAAt                                                                                                                       <  1:247946/151‑1 (MQ=255)

                               cGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCtt                                                                                                                   >  1:575424/1‑151 (MQ=255)

                                gCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCttt                                                                                                                  <  1:609716/151‑1 (MQ=255)

                                    ggcggATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTc                                                                                                              <  1:164554/151‑1 (MQ=255)

                                        gATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCAt                                                                                                          <  2:23983/151‑1 (MQ=255)

                                         aTCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCAtt                                                                                                         >  2:375407/1‑151 (MQ=255)

                                                cactATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGa                                                                                                  <  1:784127/151‑1 (MQ=255)

                                                    aTGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAAtt                                                                                               <  2:436415/150‑1 (MQ=255)

                                                     tGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTc                                                                                              >  1:237087/1‑150 (MQ=255)

                                                      ggATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTc                                                                                              >  2:496601/1‑149 (MQ=255)

                                                      ggATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAg                                                                                            <  1:292756/151‑1 (MQ=255)

                                                               gCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTc                                                                                   <  1:712804/151‑1 (MQ=255)

                                                               gCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTc                                                                                   <  2:472513/151‑1 (MQ=255)

                                                                   aaCGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAAcc                                                                               <  1:481196/151‑1 (MQ=255)

                                                                   aaCGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAAcc                                                                               >  1:42968/1‑151 (MQ=255)

                                                                   aaCGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAAcc                                                                               <  1:639103/151‑1 (MQ=255)

                                                                   aaCGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAAcc                                                                               >  1:282871/1‑151 (MQ=255)

                                                                    aCGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAAc                                                                                <  1:477619/149‑1 (MQ=255)

                                                                     cGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACcgc                                                                             <  2:363872/151‑1 (MQ=255)

                                                                       aTGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGt                                                                           <  1:91896/151‑1 (MQ=255)

                                                                          aGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGa                                                                        <  2:207839/151‑1 (MQ=255)

                                                                              gACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAAccc                                                                    <  2:469024/151‑1 (MQ=255)

                                                                                  gCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCg                                                                <  2:336490/151‑1 (MQ=255)

                                                                                   cccATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCCCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGt                                                               >  2:494055/1‑151 (MQ=255)

                                                                                     cATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCa                                                             <  2:467441/151‑1 (MQ=255)

                                                                                     cATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCAGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCa                                                             <  1:584629/151‑1 (MQ=255)

                                                                                        ccTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATc                                                          <  1:375382/151‑1 (MQ=255)

                                                                                             cGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACcgc                                                     >  1:829196/1‑151 (MQ=255)

                                                                                              gAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACcgcg                                                    <  2:569522/151‑1 (MQ=255)

                                                                                                   cATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAAc                                               <  2:69887/151‑1 (MQ=255)

                                                                                                       gACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACa                                              >  1:728543/1‑148 (MQ=255)

                                                                                                          cTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGcc                                        >  1:193181/1‑151 (MQ=255)

                                                                                                                     tCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCtcgtc                             >  1:544219/1‑151 (MQ=255)

                                                                                                                     tCAGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCtcgt                              <  2:282896/150‑1 (MQ=255)

                                                                                                                       aGTACCGTACCTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCgg                           <  2:805659/151‑1 (MQ=255)

                                                                                                                          accgtaccTGCGCCAATCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGc                         <  2:725436/150‑1 (MQ=255)

                                                                                                                                        aaTCAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCaa           >  2:236416/1‑150 (MQ=255)

                                                                                                                                           cAACGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACAGCGTCGAAACCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCAAAATg       >  1:363721/1‑151 (MQ=255)

                                                                                                                                            aaCGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCAAAATgg      <  2:622019/151‑1 (MQ=255)

                                                                                                                                            aaCGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCAAAATgg      >  2:186510/1‑151 (MQ=255)

                                                                                                                                             aCGCCTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCAAAATGGc     <  2:142777/151‑1 (MQ=255)

                                                                                                                                                ccTTATCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCAAAATGGCGAt  >  1:850827/1‑151 (MQ=255)

AGCCGGGGCAGACGGTCATGCCGTAGCCCACCGCACGGCGGATCACTTCACTATGGATATTGGGCGTAACGATGAGCTGACAGCCCATCCTGGCGAGCGCATCGACCTGTTCAGGTTTCAGTACCGTACCTGCGCCAATCAACGCCTTGTCGCCGTACGCATCAACGATGGCGGGAATGCTTTGCTCCCATTGTGGGGAATTCAGCGGGATTTCAACCGCGTCGAACCCGGCGTCAATCACCGCGCCAACATGCGCCAGCGCCTCGTCGGGCGTAATACCGCGCAAAATGGCGAT  >  NC_000913/3873291‑3873585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A