Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 283 58.3 1819865 98.8% 1798026 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,925,611 T→C D8G (GAC→GGC)  mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,925,6110TC100.0% 153.8 / NA 45D8G (GAC→GGC) mnmG5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/22);  total (23/22)

CCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTT  >  NC_000913/3925469‑3925756
                                                                                                                                              |                                                                                                                                                 
ccaaaTCCCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGGGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGa                                                                                                                                           <  2:329960/148‑1 (MQ=255)
 ccAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGAt                                                                                                                                          >  2:252070/1‑151 (MQ=255)
        cgccgATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATa                                                                                                                                   <  1:76601/151‑1 (MQ=255)
        cgccgATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATa                                                                                                                                   <  1:86219/151‑1 (MQ=255)
          ccgATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATaa                                                                                                                                  >  1:439174/1‑150 (MQ=255)
           cgATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATaaa                                                                                                                                 >  2:165902/1‑150 (MQ=255)
            gATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAAc                                                                                                                               >  2:289977/1‑151 (MQ=255)
            gATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAAc                                                                                                                               >  1:505302/1‑151 (MQ=255)
              tCGCCGGGTTGAAGCTCATCTGCCCCAGTGGGCCGATATTGTGTGTCAAACGCAGAGCCTGTTACCCCACGCGCGGCGCGGCCACCGCGACCTCGGTGCCAACACGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACa                                                                                                                              <  2:108582/150‑1 (MQ=255)
               cGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATg                                                                                                                            >  2:856947/1‑151 (MQ=255)
                   gggTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGa                                                                                                                        <  1:26888/151‑1 (MQ=255)
                      ttGCAGCTCATCTGCCCCAGAGTGGCGATATTGTGTTTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCCTCGCGGCCTCGGTGCCTGCTTGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGAtt                                                                                                                      <  1:442071/150‑1 (MQ=255)
                        gCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGcc                                                                                                                   >  1:747127/1‑151 (MQ=255)
                                   gCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATCCGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAAcg                                                                                                         <  2:787362/150‑1 (MQ=255)
                                   gCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAAcg                                                                                                         >  1:351800/1‑150 (MQ=255)
                                    ccccAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAAcgc                                                                                                        <  1:491780/150‑1 (MQ=255)
                                     cccAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCgg                                                                                                      <  2:621064/151‑1 (MQ=255)
                                      ccAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCgg                                                                                                      >  1:297515/1‑150 (MQ=255)
                                        agagTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTAt                                                                                                   <  1:692426/151‑1 (MQ=255)
                                               cGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATg                                                                                            >  2:98977/1‑151 (MQ=255)
                                                gATATTGTGTGTCAAAAGCAGAGTCTGTTGCCCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAAt                                                                                             <  1:200792/149‑1 (MQ=255)
                                                    ttGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGAtt                                                                                        <  1:551807/150‑1 (MQ=255)
                                                    ttGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGAt                                                                                         <  2:553913/149‑1 (MQ=255)
                                                    ttGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTg                                                                                       >  1:714948/1‑151 (MQ=255)
                                                     tgtgtgTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTg                                                                                       >  1:545848/1‑150 (MQ=255)
                                                              aaaGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGc                                                                             >  1:617428/1‑151 (MQ=255)
                                                               aggCAGACTCTGTCGGCCCATACGCGACGCGGCAAAGGCGGATCCGGTGCTGGTAGGACCCCCGCCAATGATGATGACGCCAAAAGGTTCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGc                                                                             <  1:658282/148‑1 (MQ=255)
                                                               aaGCGGATTCTTTTGACCCATACGCCTCGCGGCCATCGCGTCCTCGGTGCCTGCATGCCCCCCGCCAATGATGATGACGCCAAAAGGACCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGct                                                                            <  2:508416/151‑2 (MQ=255)
                                                                aGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCg                                                                           <  1:142937/151‑1 (MQ=255)
                                                                         tGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCt                                                                   >  2:588296/1‑150 (MQ=255)
                                                                             gACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTc                                                               >  2:11089/1‑150 (MQ=255)
                                                                                         ccgcGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCt                                                  >  1:148680/1‑151 (MQ=255)
                                                                                                      ggCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTg                                     <  2:155263/151‑1 (MQ=255)
                                                                                                                ccTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTggg                           >  2:772783/1‑151 (MQ=255)
                                                                                                                    cATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTAtt                       <  1:654321/151‑1 (MQ=255)
                                                                                                                     aTGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTa                      <  1:499748/151‑1 (MQ=255)
                                                                                                                          ccccGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTaaaa                   >  2:558878/1‑149 (MQ=255)
                                                                                                                                  atgatgatgACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCt          >  2:624203/1‑150 (MQ=255)
                                                                                                                                  atgatgatgACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGAATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCt          >  2:32242/1‑150 (MQ=255)
                                                                                                                                    gatgatgaCGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCtatt       <  2:629799/151‑1 (MQ=255)
                                                                                                                                    gatgatgaCGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCtat        <  2:7646/150‑1 (MQ=255)
                                                                                                                                      tgatgaCGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCtattt      >  1:415159/1‑150 (MQ=255)
                                                                                                                                      tgatgaCGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCtattt      >  2:778031/1‑150 (MQ=255)
                                                                                                                                        atgaCGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCtatttatt   <  2:237761/151‑1 (MQ=255)
                                                                                                                                          gaCGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTatttattt  >  2:227139/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
CCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTT  >  NC_000913/3925469‑3925756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A