Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 257 49.9 1554294 99.0% 1538751 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,925,611 T→C D8G (GAC→GGC)  mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,925,6110TC100.0% 159.7 / NA 47D8G (GAC→GGC) mnmG5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/26);  total (21/26)

CCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTT  >  NC_000913/3925469‑3925756
                                                                                                                                              |                                                                                                                                                 
cccAATCCCGCCGATCGCCGGGTTGCAGCGCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGa                                                                                                                                           <  2:621300/151‑1 (MQ=255)
ccaaaacccgccgATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCaaac                                                                                                                                              <  2:108122/141‑2 (MQ=255)
 caaaTCCCGCCGATCGCCGGGTGGAAGCACATCTGCCCCAGAGGGTCAATATTGTGTGTCAAAAGCAGAGCCTTTTCACCCATACCCGCCGCGGCCACCGAGGCCTCGGTCCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAg                                                                                                                                             <  1:282097/146‑1 (MQ=255)
        cgccgATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATa                                                                                                                                   <  1:497749/151‑1 (MQ=255)
          ccgATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATaaa                                                                                                                                 <  2:378947/151‑1 (MQ=255)
           cgATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATaaa                                                                                                                                 >  2:741608/1‑150 (MQ=255)
             aTCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAAc                                                                                                                               >  1:62070/1‑150 (MQ=255)
                gCCGGGTTGCAGCTCTTCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATgg                                                                                                                           >  2:767395/1‑151 (MQ=255)
                gCCGGGTTGCAGCGCATCTGCCCGAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATTGCGGTCTCGGTGCATGCATGACCCCCGCCAATGATGATGGAGCAAAAAGGCTCCGGATAAAACAt                                                                                                                             >  1:258705/1‑149 (MQ=255)
                   gggTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATTGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTg                                                                                                                         <  2:97555/150‑1 (MQ=255)
                   gggTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTg                                                                                                                         >  1:393777/1‑150 (MQ=255)
                   gggTTGCAGCTCATCTGCCCCAGAGGGGCGATTTTGTGTGTCAAAAGCAGAGTCGGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTg                                                                                                                         <  1:529599/150‑1 (MQ=255)
                          aGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTc                                                                                                                 <  2:758670/151‑1 (MQ=255)
                           gCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCg                                                                                                                >  1:26730/1‑151 (MQ=255)
                            cccATGTGCCCCAGGGGGTGGGGATTGGGTGGCAAAAGAAGAGGCTGTGGACCCATACGCGCCGCGGCCAACGCGGCCTCGGTGCCAGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCt                                                                                                                  <  1:460035/146‑1 (MQ=255)
                             tCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGc                                                                                                               >  2:524986/1‑150 (MQ=255)
                                    ccccAGAGTGTCGATATTGTGGGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAAcgc                                                                                                        <  1:297684/150‑1 (MQ=255)
                                      ccAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGt                                                                                                     >  2:750438/1‑151 (MQ=255)
                                              tCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGaaa                                                                                               <  2:535413/149‑1 (MQ=255)
                                               cGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATg                                                                                            >  2:201475/1‑151 (MQ=255)
                                                 atatTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATgg                                                                                           >  2:91990/1‑150 (MQ=255)
                                                  tatTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGAt                                                                                         >  1:97624/1‑151 (MQ=255)
                                                    ttGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTg                                                                                       >  2:657314/1‑151 (MQ=255)
                                                            cAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCgg                                                                               >  2:637770/1‑151 (MQ=255)
                                                             aaaacaagagTCTATGCACCCAGACGCGCCGCGCCACCCGCGGCGCAGTGGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGcc                                                                                  <  2:44874/141‑1 (MQ=255)
                                                             aaaaGCAGAGTCTGTTGACCAATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGccc                                                                                 <  1:653318/148‑1 (MQ=255)
                                                               aggCAGAGTCTGTTGCCCCATACGCGCCGCGGCCATCGCGGCCCCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGcc                                                                            <  2:760507/149‑1 (MQ=255)
                                                                    gagTCTGTTGCCCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGCCCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTgg                                                                        <  1:494254/150‑1 (MQ=255)
                                                                    gagTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTgg                                                                        >  1:239957/1‑150 (MQ=255)
                                                                     agTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGa                                                                       <  2:543008/150‑1 (MQ=255)
                                                                            tGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACt                                                                <  2:9929/150‑1 (MQ=255)
                                                                             gACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTc                                                               >  2:709016/1‑150 (MQ=255)
                                                                                     cgcgCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTc                                                      <  2:193642/151‑1 (MQ=255)
                                                                                     cgcgCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTc                                                      <  2:762324/151‑1 (MQ=255)
                                                                                      gcgcCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCg                                                     <  2:393826/151‑1 (MQ=255)
                                                                                                cATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGaa                                            >  2:752233/1‑150 (MQ=255)
                                                                                                   cgcgGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGa                                         <  2:34599/150‑1 (MQ=255)
                                                                                                   cgcgGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGAc                                        >  2:664233/1‑151 (MQ=255)
                                                                                                      ggCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTg                                     <  2:307240/151‑1 (MQ=255)
                                                                                                                ccTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTggg                           >  1:18075/1‑151 (MQ=255)
                                                                                                                 gtGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTgg                            <  1:379873/148‑1 (MQ=255)
                                                                                                                 cTGCATGACCCCCGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTggg                           >  1:96865/1‑150 (MQ=255)
                                                                                                                          ccccGCCAATGATGATGACGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTaa                     <  1:311687/147‑1 (MQ=255)
                                                                                                                                     atgatgaCGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCtattt      >  1:32730/1‑151 (MQ=255)
                                                                                                                                      tgatgaCGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCtattt      >  2:182785/1‑150 (MQ=255)
                                                                                                                                       gatgaCGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCtattta     <  1:522347/150‑1 (MQ=255)
                                                                                                                                          gaCGCCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTatttattt  <  1:655913/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
CCCAATACCGCCGATCGCCGGGTTGCAGCTCATCTGCCCCAGAGTGTCGATATTGTGTGTCAAAAGCAGAGTCTGTTGACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGCCTGCATGACCCCCGCCAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTT  >  NC_000913/3925469‑3925756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A