Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 283 58.3 1819865 98.8% 1798026 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,586,594 (G)6→5 coding (168/3513 nt) hsdR ← endonuclease R Type I restriction enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,586,5890G.100.0% 258.3 / NA 54coding (173/3513 nt)hsdRendonuclease R Type I restriction enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (25/29);  total (25/29)

GGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGG  >  NC_000913/4586449‑4586735
                                                                                                                                            |                                                                                                                                                  
gggCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAgggggatg                                                                                                                                             <  2:800154/148‑4 (MQ=255)
    aTCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGt                                                                                                                                      >  1:831899/1‑150 (MQ=255)
    aTCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGt                                                                                                                                      >  1:636501/1‑150 (MQ=255)
      cGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTaa                                                                                                                                    <  2:590087/150‑1 (MQ=255)
         tGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAg                                                                                                                                 <  2:26510/150‑1 (MQ=255)
         tGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAg                                                                                                                                 <  1:767785/150‑1 (MQ=255)
         tGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAg                                                                                                                                 <  1:443008/150‑1 (MQ=255)
           agaTCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGAcc                                                                                                                              >  1:219108/1‑151 (MQ=255)
               cGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGa                                                                                                                          <  1:262132/151‑1 (MQ=255)
                 ttATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATg                                                                                                                        >  1:121995/1‑151 (MQ=255)
                 ttATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATg                                                                                                                        >  1:354567/1‑151 (MQ=255)
                       tatTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGc                                                                                                                  >  1:798676/1‑151 (MQ=255)
                        gttCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGGGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGAAACAGACCAAGATGTATAGCt                                                                                                                 >  2:103024/2‑151 (MQ=255)
                               cACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCtt                                                                                                          <  2:9331/151‑1 (MQ=255)
                                aCCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTc                                                                                                         <  1:1172/151‑1 (MQ=255)
                                aCCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTc                                                                                                         <  1:631579/151‑1 (MQ=255)
                                  cGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGc                                                                                                       >  1:464709/1‑151 (MQ=255)
                                    ccTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCa                                                                                                     <  1:204921/151‑1 (MQ=255)
                                       ggTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCaaa                                                                                                   <  2:563859/150‑1 (MQ=255)
                                        gTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACa                                                                                                 <  2:630966/151‑1 (MQ=255)
                                          tACCAATGCGGCGTAATTTTTGAAATACAGAGAGGATGTTGGCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCaa                                                                                                    >  2:346266/1‑146 (MQ=255)
                                          tACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACAt                                                                                                >  2:689644/1‑150 (MQ=255)
                                            ccAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACg                                                                                             >  2:325069/1‑151 (MQ=255)
                                                 gcggcgTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCAtt                                                                                         <  2:202115/150‑1 (MQ=255)
                                                  cggcgTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCAtttt                                                                                       >  1:698993/1‑151 (MQ=255)
                                                    gcgTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTaa                                                                                     <  2:15/151‑1 (MQ=255)
                                                      gTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATc                                                                                   <  2:495150/151‑1 (MQ=255)
                                                         aTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGc                                                                                <  1:545748/151‑1 (MQ=255)
                                                                aaaTACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCgtgt                                                                         >  1:801084/1‑151 (MQ=255)
                                                                         gagGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGatcat                                                                >  2:112081/1‑151 (MQ=255)
                                                                          agGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGatcatc                                                               >  2:763105/1‑151 (MQ=255)
                                                                             aTGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCgg                                                            <  2:244103/151‑1 (MQ=255)
                                                                               gttgtCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGt                                                          >  1:242948/1‑151 (MQ=255)
                                                                                 tgtCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGt                                                          >  1:463246/1‑149 (MQ=255)
                                                                                    catcaACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGtt                                                      >  1:436103/1‑150 (MQ=255)
                                                                                    catcaACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTa                                                     <  1:420614/151‑1 (MQ=255)
                                                                                       caacaaAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTAttt                                                  <  1:804245/151‑1 (MQ=255)
                                                                                       caacaaAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTAtt                                                   <  1:132481/150‑1 (MQ=255)
                                                                                                cGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCcgccgc                                          >  2:871620/1‑150 (MQ=255)
                                                                                                 gATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGcac                                        <  2:831832/151‑1 (MQ=255)
                                                                                                  aTTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGcac                                        >  1:441154/1‑150 (MQ=255)
                                                                                                        ccGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGAt                                 <  1:112103/151‑1 (MQ=255)
                                                                                                               cACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATa                           >  2:907141/1‑150 (MQ=255)
                                                                                                                  gCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTg                       >  1:284631/1‑151 (MQ=255)
                                                                                                                    aGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGa                      >  2:462935/1‑150 (MQ=255)
                                                                                                                       agaTCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTc                  <  1:852297/151‑1 (MQ=255)
                                                                                                                       agaTCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTc                  >  1:446525/1‑151 (MQ=255)
                                                                                                                            gtgtTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTg              <  2:108176/150‑1 (MQ=255)
                                                                                                                            gtgtTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTg              >  1:706924/1‑150 (MQ=255)
                                                                                                                            gtgtTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGa             <  2:706875/151‑1 (MQ=255)
                                                                                                                            gtgtTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGa             <  2:909797/151‑1 (MQ=255)
                                                                                                                              gtTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGAc            <  1:876612/150‑1 (MQ=255)
                                                                                                                                  aTTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCt       <  1:849486/151‑1 (MQ=255)
                                                                                                                                   ttCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGTGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGCTGGCATAAGTGAAGTCGTTGACGCCCt       <  2:287262/150‑1 (MQ=255)
                                                                                                                                       cacaA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAgg  <  1:457763/151‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
GGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGG  >  NC_000913/4586449‑4586735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A