Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I1 R1 300 54.7 1718999 98.4% 1691495 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,586,594 (G)6→5 coding (168/3513 nt) hsdR ← endonuclease R Type I restriction enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,586,5890G.100.0% 199.9 / NA 42coding (173/3513 nt)hsdRendonuclease R Type I restriction enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (20/22);  total (20/22)

GGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAG  >  NC_000913/4586440‑4586734
                                                                                                                                                     |                                                                                                                                                 
ggCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAgg                                                                                                                                                  >  1:632506/1‑151 (MQ=255)
ggCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAg                                                                                                                                                   <  2:396911/150‑1 (MQ=255)
  cacaTCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAgggg                                                                                                                                                <  2:108055/151‑1 (MQ=255)
    caTCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAggggg                                                                                                                                               >  2:291863/1‑150 (MQ=255)
     aTCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAgggggat                                                                                                                                             >  1:567861/1‑149 (MQ=255)
        tGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGtt                                                                                                                                         >  2:56263/1‑151 (MQ=255)
          ggCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGa                                                                                                                                       <  2:72015/151‑1 (MQ=255)
                  tGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAAc                                                                                                                                  >  2:222359/1‑148 (MQ=255)
                  tGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAAc                                                                                                                                  >  2:135521/1‑148 (MQ=255)
                  tGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAAc                                                                                                                                  >  1:119925/1‑148 (MQ=255)
                        cGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAg                                                                                                                          <  1:844906/150‑1 (MQ=255)
                              gATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGttgc                                                                                                                   <  1:279921/151‑3 (MQ=255)
                                    cGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCtgtg                                                                                                             >  2:221286/1‑151 (MQ=255)
                                       gCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGc                                                                                                           <  2:135563/150‑1 (MQ=255)
                                         aCCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCtt                                                                                                         >  2:194432/1‑150 (MQ=255)
                                            gCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGcc                                                                                                     >  1:463010/1‑151 (MQ=255)
                                            gCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGcc                                                                                                     >  2:554801/1‑151 (MQ=255)
                                                ggTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCaa                                                                                                   <  1:520224/149‑1 (MQ=255)
                                                   tACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACAt                                                                                               >  2:484819/1‑150 (MQ=255)
                                                     ccAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACg                                                                                            <  1:606256/151‑1 (MQ=255)
                                                       aaTGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCa                                                                                          <  2:632546/151‑1 (MQ=255)
                                                             gcgTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTaa                                                                                    <  1:739900/151‑1 (MQ=255)
                                                             gcgTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTaa                                                                                    <  1:316452/151‑1 (MQ=255)
                                                               gTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATc                                                                                  <  1:777744/151‑1 (MQ=255)
                                                                 aaTTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCa                                                                                 <  1:829295/150‑1 (MQ=255)
                                                                      gtgAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGcgtc                                                                            <  2:593612/150‑1 (MQ=255)
                                                                           aTACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTg                                                                      >  1:656859/1‑151 (MQ=255)
                                                                              cAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGa                                                                   <  2:732623/151‑1 (MQ=255)
                                                                                gagagGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGat                                                                  >  2:104638/1‑150 (MQ=255)
                                                                                 agagGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGTAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGatc                                                                 >  2:707820/1‑150 (MQ=255)
                                                                                        gttgtCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGt                                                         >  2:414176/1‑151 (MQ=255)
                                                                                          tgtCATCAACAAAGGCGATTTTGCCGATTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGAGGTTGTGTAACATACCAAGATGTTTCGCTGTGGCTTGGCCAAACATACGCTTTTTAATCAGCGTCGTGTTGGGATCATCCGGGt                                                         >  1:421887/1‑149 (MQ=255)
                                                                                            tcatcaACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGtt                                                     >  1:21115/1‑151 (MQ=255)
                                                                                              atcaACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTAt                                                   <  1:554763/151‑1 (MQ=255)
                                                                                                  acaaAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTc                                               >  2:495125/1‑151 (MQ=255)
                                                                                                   caaAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTcc                                              <  2:15114/151‑1 (MQ=255)
                                                                                                     aaGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTccgc                                            >  1:163554/1‑151 (MQ=255)
                                                                                                           aTTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGcac                                       >  1:444434/1‑150 (MQ=255)
                                                                                                                 ccGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGAt                                <  2:187706/151‑1 (MQ=255)
                                                                                                                 ccGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGAt                                <  2:724427/151‑1 (MQ=255)
                                                                                                                  cGAGTTCACGCAGGAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATg                               <  1:709144/151‑1 (MQ=255)
                                                                                                                              ggAGATCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAg                   <  1:222335/151‑1 (MQ=255)
                                                                                                                                agaTCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTc                 <  2:599584/151‑1 (MQ=255)
                                                                                                                                   tCGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGtt              >  1:755525/1‑151 (MQ=255)
                                                                                                                                    cGTGTTGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGtt              <  1:28879/150‑1 (MQ=255)
                                                                                                                                         tGATTCTCACAA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGcc        >  2:579286/1‑151 (MQ=255)
                                                                                                                                                cacaA‑GGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAg  <  1:527276/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAG  >  NC_000913/4586440‑4586734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A