Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,998,412	T→C	W144R (TGG→CGG)	uvrD →	DNA‑dependent ATPase I and helicase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,998,412	0	T	C	100.0%	141.9 / NA	40	W144R (TGG→CGG)	uvrD	DNA‑dependent ATPase I and helicase II
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/19); total (21/19)

GTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGAAGCGTGTGACCGCGCGGGCCTGGTGGACTTCGCCGAG  >  NC_000913/3998269‑3998555

gTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACAt                                                                                                                                          >  1:517831/1‑151 (MQ=255)

    gctgcGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAAc                                                                                                                                      >  2:688973/1‑151 (MQ=255)

       gcgtgcgCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACa                                                                                                                                     <  1:430150/149‑1 (MQ=255)

         gtgcgCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCa                                                                                                                                 <  2:376698/151‑1 (MQ=255)

             gcACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGaa                                                                                                                              >  2:432072/1‑150 (MQ=255)

                 cATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATg                                                                                                                         <  2:207018/151‑1 (MQ=255)

                   tatGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGa                                                                                                                        >  1:207680/1‑150 (MQ=255)

                        aCGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGcc                                                                                                                   <  1:656362/150‑1 (MQ=255)

                             aaTCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTc                                                                                                             <  2:150195/151‑1 (MQ=255)

                               tCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCg                                                                                                           >  1:741099/1‑151 (MQ=255)

                                cTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGc                                                                                                          >  1:124504/1‑151 (MQ=255)

                                   ccGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGcatc                                                                                                       <  2:479760/151‑1 (MQ=255)

                                     gCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCATAGCCATGAACCTCGACTAGAAGCAGTGGTCGCCGCGGCACGCAATGCGGTACATCAACAGCCAGCAAGATGAAGGCCTGCCTCCGcatcat                                                                                                     >  1:744669/1‑151 (MQ=255)

                                                  aTCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACg                                                                                        >  1:492615/1‑151 (MQ=255)

                                                     cTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACgg                                                                                       >  1:256666/1‑149 (MQ=255)

                                                                 gACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGc                                                                         <  2:409547/151‑1 (MQ=255)

                                                                  aCCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGc                                                                         >  2:625885/1‑150 (MQ=255)

                                                                   ccAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGACGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGc                                                                         >  1:444007/1‑149 (MQ=255)

                                                                   ccAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAg                                                                       <  2:573356/151‑1 (MQ=255)

                                                                         aCGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCt                                                                   <  1:599516/149‑1 (MQ=255)

                                                                         aCGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTgg                                                                 <  2:114518/151‑1 (MQ=255)

                                                                               gCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGaa                                                            <  2:560452/150‑1 (MQ=255)

                                                                                  tAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGgtg                                                        >  2:236170/1‑151 (MQ=255)

                                                                                   aaGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGgtgt                                                       <  2:637466/151‑1 (MQ=255)

                                                                                   aaGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGgtgt                                                       <  2:741173/151‑1 (MQ=255)

                                                                                          tGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGc                                                >  2:341027/1‑151 (MQ=255)

                                                                                              cAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTAt                                            >  1:108419/1‑151 (MQ=255)

                                                                                               aaaGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATc                                           >  1:265257/1‑151 (MQ=255)

                                                                                                    cATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGaa                                      <  1:93114/151‑1 (MQ=255)

                                                                                                     aTGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGa                                       >  1:144448/1‑149 (MQ=255)

                                                                                                          ccTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGAAGCgtgt                                >  2:716074/1‑151 (MQ=255)

                                                                                                           cTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGAAGCGtgtg                               >  2:308837/1‑151 (MQ=255)

                                                                                                               acgaGAAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGAAGCGTGTGACcg                           <  2:317484/151‑1 (MQ=255)

                                                                                                                 gagaAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGAAGCGTGTGACcgcg                         >  2:14163/1‑151 (MQ=255)

                                                                                                                 gagaAGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGAATCAGGAAGCGTGTGACcg                           >  1:328989/1‑149 (MQ=255)

                                                                                                                    aaGCAGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGAAGCGTGTGACCGCGCgg                      >  1:774833/1‑151 (MQ=255)

                                                                                                                        aGTGGCCGCCGCGGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGAAGCGTGTGACCGCGCGGGCCt                  <  2:619254/151‑1 (MQ=255)

                                                                                                                                 cgcgGCAGGCAATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGAAGCGTGTGACCGCGCGGGCCTGGTGGACtt         <  2:620731/151‑1 (MQ=255)

                                                                                                                                      caggcaATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGAAGCGTGTGACCGCGCGGGCCTGGTGGACTTCGCCg    <  1:624465/151‑1 (MQ=255)

                                                                                                                                        ggcaATGCGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGAAGCGTGTGACCGCGCGGGCCTGGTGGACTTCGCCGAg  <  1:487838/151‑1 (MQ=255)

GTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAAAGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGAAGCGTGTGACCGCGCGGGCCTGGTGGACTTCGCCGAG  >  NC_000913/3998269‑3998555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A