Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 257 49.9 1554294 99.0% 1538751 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,014,886 A→G S612G (AGC→GGC)  metE → 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,014,8860AG100.0% 150.1 / NA 43S612G (AGC→GGC) metE5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/19);  total (24/19)

GTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGAC  >  NC_000913/4014738‑4015032
                                                                                                                                                    |                                                                                                                                                  
gTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGAT‑‑AGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCg                                                                                                                                                 >  1:82465/1‑150 (MQ=255)
     cgcgTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTg                                                                                                                                             <  1:20396/151‑1 (MQ=255)
      gcgTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTgg                                                                                                                                            >  2:304570/1‑151 (MQ=255)
                gTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTAtc                                                                                                                                  <  1:438080/151‑1 (MQ=255)
                  cAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTAtctc                                                                                                                                <  2:182401/151‑1 (MQ=255)
                    gCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCa                                                                                                                              <  1:628473/151‑1 (MQ=255)
                             ccATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGt                                                                                                                     >  2:305462/1‑151 (MQ=255)
                              cATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTa                                                                                                                    <  2:190976/151‑1 (MQ=255)
                               aTCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTAGCTCCAGTGGGGCGTag                                                                                                                   <  1:85482/151‑1 (MQ=255)
                                     aaaCAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCt                                                                                                             >  1:130638/1‑151 (MQ=255)
                                           aTTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGACGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGt                                                                                                        >  1:714805/1‑150 (MQ=255)
                                                    gcgctgCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAAcgccg                                                                                              >  2:6019/1‑151 (MQ=255)
                                                    gcgctgCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAAcgc                                                                                                <  1:424459/149‑1 (MQ=255)
                                                      gctgCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACgccgcc                                                                                            >  1:346877/1‑151 (MQ=255)
                                                         gCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGccgccg                                                                                           <  2:752305/149‑1 (MQ=255)
                                                               tgaAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGaa                                                                                    >  2:502359/1‑150 (MQ=255)
                                                                gaAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGaaa                                                                                   <  1:531259/150‑1 (MQ=255)
                                                                        cGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGacac                                                                           >  2:206128/1‑150 (MQ=255)
                                                                             tGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAAt                                                                     >  1:523721/1‑151 (MQ=255)
                                                                               gAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATcc                                                                   >  2:368306/1‑151 (MQ=255)
                                                                               gAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATcc                                                                   <  2:742580/151‑1 (MQ=255)
                                                                                aaGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATcc                                                                   >  1:543716/1‑150 (MQ=255)
                                                                                 aGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCcac                                                                 <  2:109391/151‑1 (MQ=255)
                                                                                 aGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCca                                                                  >  2:688537/1‑150 (MQ=255)
                                                                                  gCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCcaca                                                                <  1:144125/151‑1 (MQ=255)
                                                                                       tGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTcac                                                           >  1:768538/1‑151 (MQ=255)
                                                                                       tGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTcac                                                           >  1:96821/1‑151 (MQ=255)
                                                                                            ttGGCATAATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACAtgtg                                                      >  1:742171/1‑151 (MQ=255)
                                                                                               gCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTa                                                   <  2:769783/151‑1 (MQ=255)
                                                                                                catcatCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTAt                                                  >  2:298924/1‑151 (MQ=255)
                                                                                                   catcCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGc                                               >  1:289868/1‑151 (MQ=255)
                                                                                                                    cGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGAcatcat                              <  2:655320/151‑1 (MQ=255)
                                                                                                                          tGCGCGAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTc                        >  1:498407/1‑151 (MQ=255)
                                                                                                                             gcgAAGGTTTACCGCTGCGTAGTGGCGCCTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGAt                     >  2:538633/1‑151 (MQ=255)
                                                                                                                               gAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGa                      <  2:214980/148‑1 (MQ=255)
                                                                                                                               gAAGGTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGa                      >  1:214948/1‑148 (MQ=255)
                                                                                                                                  ggTTTACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTgcgg                <  1:663581/151‑1 (MQ=255)
                                                                                                                                      tACCGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCt            >  1:137236/1‑151 (MQ=255)
                                                                                                                                         cGCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGa         <  2:137269/151‑1 (MQ=255)
                                                                                                                                         cGCTGCGTCGTGGCGACGGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTgg          >  1:766845/1‑150 (MQ=255)
                                                                                                                                          gCTGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGAc        <  2:389010/151‑1 (MQ=255)
                                                                                                                                            tGCGTCGTGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGc      >  2:415416/1‑151 (MQ=255)
                                                                                                                                                 cgtGGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGAc  <  2:703071/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGAC  >  NC_000913/4014738‑4015032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A