Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,306,735	A→G	intergenic (‑138/+135)	yjcS ← / ← alsK	metallo‑beta‑lactamase superfamily protein/D‑allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,306,735	0	A	G	100.0%	171.2 / NA	48	intergenic (‑138/+135)	yjcS/alsK	metallo‑beta‑lactamase superfamily protein/D‑allose kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/23); total (25/23)

AGTTATTCATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCATTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGG  >  NC_000913/4306588‑4306877

agTTATTCATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGttt                                                                                                                                             >  1:695080/1‑151 (MQ=255)

  ttATTCATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTtg                                                                                                                                            >  2:385144/1‑150 (MQ=255)

   tATTCATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTtgtg                                                                                                                                          >  1:506794/1‑151 (MQ=255)

     ttCATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCt                                                                                                                                        >  1:726414/1‑151 (MQ=255)

       cATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTg                                                                                                                                       >  2:586940/1‑150 (MQ=255)

             aaTCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAggg                                                                                                                                <  1:596114/151‑1 (MQ=255)

                 tcCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGaaa                                                                                                                             >  2:489140/1‑150 (MQ=255)

                  ccATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAAt                                                                                                                           >  1:94373/1‑151 (MQ=255)

                       tatcatttatGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACa                                                                                                                       <  2:19831/150‑1 (MQ=255)

                          catttatGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTaa                                                                                                                   <  2:355169/151‑1 (MQ=255)

                               atGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACt                                                                                                               <  2:132472/150‑1 (MQ=255)

                                tGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTAt                                                                                                             >  2:376983/1‑151 (MQ=255)

                                 gTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTAt                                                                                                             <  2:489617/150‑1 (MQ=255)

                                    gTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCAtt                                                                                                         <  2:505031/151‑1 (MQ=255)

                                    gTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCAtt                                                                                                         <  2:91275/151‑1 (MQ=255)

                                      aaaTATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGc                                                                                                       >  2:23616/1‑151 (MQ=255)

                                      aaaTATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGc                                                                                                       <  2:445138/151‑1 (MQ=255)

                                      aaaTATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGc                                                                                                       >  2:121471/1‑151 (MQ=255)

                                            gTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGaaaa                                                                                                 <  2:742951/151‑1 (MQ=255)

                                                  cATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAaataat                                                                                             <  1:589278/149‑1 (MQ=255)

                                                   aTGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATg                                                                                          >  2:424297/1‑151 (MQ=255)

                                                     gCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAg                                                                                        <  2:744235/151‑1 (MQ=255)

                                                         aaTACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGt                                                                                    <  2:411911/151‑1 (MQ=255)

                                                          aTACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGtt                                                                                   >  2:40752/1‑151 (MQ=255)

                                                            aCGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGtttt                                                                                 <  1:13559/151‑1 (MQ=255)

                                                            aCGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGtttt                                                                                 <  1:557623/151‑1 (MQ=255)

                                                                cATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTAtgt                                                                             >  1:444993/1‑151 (MQ=255)

                                                                cATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTAtg                                                                              <  2:254858/150‑1 (MQ=255)

                                                                cATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTAtg                                                                              <  1:424246/150‑1 (MQ=255)

                                                                 aTTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTAtgtg                                                                            >  2:152852/1‑151 (MQ=255)

                                                                  tttAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATgtgt                                                                           >  1:557316/1‑151 (MQ=255)

                                                                  tttAAAGCTGGCGGTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGAAAGTGCTGAAGAGGGAAAATTACAGTAAAACTATCACTGCAGAAAATACTATGAGTCGATTTAt                                                                               <  1:311994/147‑1 (MQ=255)

                                                                   ttAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGtt                                                                          >  2:239418/1‑151 (MQ=255)

                                                                     aaaGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGttt                                                                         <  2:301624/150‑1 (MQ=255)

                                                                        gCTGGCGTTATCATAATTGGGAAATCGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTttatta                                                                     <  1:384814/151‑1 (MQ=255)

                                                                            gCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATtatat                                                                  >  1:590971/1‑150 (MQ=255)

                                                                             cGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATtatatat                                                                >  2:361490/1‑151 (MQ=255)

                                                                                    aTAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTAc                                                         >  2:404170/1‑151 (MQ=255)

                                                                                          gggTAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGt                                                     >  1:513977/1‑149 (MQ=255)

                                                                                          gggAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCg                                                   >  1:164926/1‑151 (MQ=255)

                                                                                            gAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGtt                                                 >  1:469065/1‑151 (MQ=255)

                                                                                                  gCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGcac                                            <  2:164959/150‑1 (MQ=255)

                                                                                                    gACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGc                                         >  2:209131/1‑151 (MQ=255)

                                                                                                          aataaGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACa                                   <  2:237556/151‑1 (MQ=255)

                                                                                                             aaGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCGTTTGTGCTGCAAATGGACAATTACAGTAAAACTATCCTTGCAGAAAATAATATGACTCGTTTTATGTGTCTATTCTCTATATTGGACCTGTCGTTGGCACAGAGGGACATTg                                >  1:693572/1‑151 (MQ=255)

                                                                                                                                   cgcCATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTAc          >  2:93032/1‑151 (MQ=255)

                                                                                                                                     ccATTTTATTAGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTc        <  1:152819/151‑1 (MQ=255)

                                                                                                                                            attaGGCGTTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATgggg  <  2:618847/150‑1 (MQ=255)

AGTTATTCATATAAATCTCCATTTATCATTTATGTCGTAAATATGTAATCCATGCAAAATACGTCATTTAAAGCTGGCGTTATCATAATTGGGAAATTGCGACTTTAATAAGTGGAAGTGTGAGCGGAACGCGCCATTTTATTAGGCATTTGTGCTGAAGAGGGAAAATTACAGTAAAACTATCATTGCAGAAAATAATATGAGTCGTTTTATGTGTTTATTATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGG  >  NC_000913/4306588‑4306877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A