Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 257 49.9 1554294 99.0% 1538751 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,485,806 Δ1 bp coding (146/1512 nt) pepA ← multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,485,8030C.100.0% 204.0 / NA 43coding (149/1512 nt)pepAmultifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (30/13);  total (30/13)

GGTTTTCTGAATAACCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAA  >  NC_000913/4485664‑4485944
                                                                                                                                           |                                                                                                                                             
ggTTTTCTGAATAACCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCa                                                                                                                                   <  1:131303/151‑1 (MQ=255)
   tttCTGAATAACCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAggg                                                                                                                                >  1:293227/1‑151 (MQ=255)
          aTAACCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGAt                                                                                                                         >  1:174072/1‑151 (MQ=255)
            aaCCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGt                                                                                                                       <  2:293513/151‑1 (MQ=255)
            aaCCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGt                                                                                                                       >  2:442296/1‑151 (MQ=255)
            aaCCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGt                                                                                                                       >  2:561609/1‑151 (MQ=255)
               cTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTAcc                                                                                                                    >  2:711796/1‑151 (MQ=255)
                       aCTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCt                                                                                                             <  2:244033/150‑1 (MQ=255)
                           aCGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGAttt                                                                                                        >  2:16458/1‑151 (MQ=255)
                                  tCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCg                                                                                                   >  2:723041/1‑149 (MQ=255)
                                        tCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTc                                                                                           >  2:767794/1‑151 (MQ=255)
                                         cACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGtt                                                                                            <  1:754981/149‑1 (MQ=255)
                                         cACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCt                                                                                          <  2:590831/151‑1 (MQ=255)
                                            gTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGc                                                                                        >  2:216605/1‑150 (MQ=255)
                                            gTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGc                                                                                        >  1:264819/1‑150 (MQ=255)
                                               cTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATc                                                                                    >  1:419242/1‑151 (MQ=255)
                                                    ccGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCgg                                                                                  >  1:655619/1‑148 (MQ=255)
                                                          ccAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGc                                                                          <  1:183518/150‑1 (MQ=255)
                                                               aaGGGGAATTCGCTCGGACAGTAAATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTGCG‑CCCGACGTAGCAGTGCGCTGATGTACCCCTCGCAGATATAATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGt                                                                     >  1:200453/1‑150 (MQ=255)
                                                                     aaTTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCg                                                               >  2:626173/1‑150 (MQ=255)
                                                                     aaTTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCg                                                               >  1:222822/1‑150 (MQ=255)
                                                                        tCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAg                                                            <  1:546194/150‑1 (MQ=255)
                                                                         cGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGAc                                                          >  2:757922/1‑151 (MQ=255)
                                                                                     aCATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGAt                                              >  2:449832/1‑151 (MQ=255)
                                                                                      cATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATg                                             <  2:305289/151‑1 (MQ=255)
                                                                                         tCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCa                                           <  1:458069/150‑1 (MQ=255)
                                                                                          cGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAgg                                         >  1:144395/1‑151 (MQ=255)
                                                                                           ggAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGc                                        >  2:638844/1‑151 (MQ=255)
                                                                                           ggAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGc                                        >  1:151522/1‑151 (MQ=255)
                                                                                             aaCATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCAc                                      <  1:745424/151‑1 (MQ=255)
                                                                                              aCATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCGCACGACGATGCAGGCACt                                     <  1:710744/151‑1 (MQ=255)
                                                                                                aTGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTcc                                   >  2:664612/1‑151 (MQ=255)
                                                                                                aTGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTcc                                   >  1:231058/1‑151 (MQ=255)
                                                                                                  ggTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGc                                 >  1:428471/1‑151 (MQ=255)
                                                                                                     gcagcaACAATGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTg                               <  2:419293/150‑1 (MQ=255)
                                                                                                             aaTGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCgg                      >  1:558890/1‑151 (MQ=255)
                                                                                                               tGTCTGCCCCGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGc                    >  1:44885/1‑151 (MQ=255)
                                                                                                                       ccGGTTTTCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCtt            >  1:165910/1‑151 (MQ=255)
                                                                                                                           ttttCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCttttt         >  2:372325/1‑150 (MQ=255)
                                                                                                                           ttttCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCttttt         <  1:626116/150‑1 (MQ=255)
                                                                                                                           ttttCCTTCCAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCtttt          >  1:471117/1‑149 (MQ=255)
                                                                                                                               ccttccAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACt    >  1:717535/1‑151 (MQ=255)
                                                                                                                                  tccAGTTCG‑CCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTaa  >  1:509689/1‑150 (MQ=255)
                                                                                                                                           |                                                                                                                                             
GGTTTTCTGAATAACCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAA  >  NC_000913/4485664‑4485944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A