Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	11	44.6	1622634	97.7%	1585313	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,128,361	T→C	Y12C (TAC→TGC)	metJ ←	transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,128,361	0	T	C	100.0%	147.4 / NA	40	Y12C (TAC→TGC)	metJ	transcriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/22); total (18/22)

TCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGT  >  NC_000913/4128217‑4128504

tcccacaGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGCGCGATCACCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGc                                                                                                                                           >  1:111162/4‑151 (MQ=255)

    acaGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGat                                                                                                                                       <  2:240520/151‑1 (MQ=255)

       gcagcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGatat                                                                                                                                     <  1:76452/150‑1 (MQ=255)

         agcTCGCTGTTGGTAGCGTGACGCAGTTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGATGAATGGAAACCGTAAACTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATAtt                                                                                                                                  <  1:635615/151‑1 (MQ=255)

          gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTc                                                                                                                                 >  2:358443/1‑151 (MQ=255)

          gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTc                                                                                                                                 <  2:128684/151‑1 (MQ=255)

             cGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCTTGCGTTCATCGGTGATGATTTTTAACACCGTAAGAGGAATGGAAACCGTAGTCTTTTTGATTTGTTCGCTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTcgcc                                                                                                                              <  1:542524/151‑1 (MQ=255)

                 gTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCt                                                                                                                           <  2:451161/150‑1 (MQ=255)

                                      gttCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACtt                                                                                                      <  2:386334/150‑1 (MQ=255)

                                               acgacgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCtt                                                                                             <  2:387727/150‑1 (MQ=255)

                                               acgacgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTc                                                                                            <  1:706944/151‑1 (MQ=255)

                                                 gacgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCg                                                                                           <  1:331446/150‑1 (MQ=255)

                                                 gacgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGt                                                                                          <  1:157570/151‑1 (MQ=255)

                                                    gcgtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCa                                                                                        >  1:450788/1‑150 (MQ=255)

                                                     cgtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAAt                                                                                      <  1:239509/151‑1 (MQ=255)

                                                       tgcgtTCATCGGTGAGGATTTTTAACACCTTAAGATGAATGGAAACCGTAATCTTTTTGTCTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATAGATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAAt                                                                                      <  1:352621/149‑1 (MQ=255)

                                                                 ggTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacca                                                                          <  1:715607/151‑1 (MQ=255)

                                                                 ggTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacca                                                                          <  2:21016/151‑1 (MQ=255)

                                                                 ggTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacc                                                                           >  2:449576/1‑150 (MQ=255)

                                                                       gATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACcac                                                                    >  2:351651/1‑151 (MQ=255)

                                                                               acacCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGa                                                            <  1:386024/151‑1 (MQ=255)

                                                                                cacCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAt                                                           <  1:767568/151‑1 (MQ=255)

                                                                                    ttAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCAAGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTgg                                                       >  2:216115/1‑151 (MQ=255)

                                                                                     tAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTggg                                                      >  2:338994/1‑151 (MQ=255)

                                                                                       agagGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTa                                                    <  2:62969/151‑1 (MQ=255)

                                                                                             aTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGAcc                                              <  2:663488/151‑1 (MQ=255)

                                                                                             aTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGAcc                                              <  2:861/151‑1 (MQ=255)

                                                                                               tgAACCCGTAATCTTTTTGACATGCTCACTCTTCTTGCCGTGATCAGAGCATGGGCTGATATATTCGCCGCTACATTCAGCCATGAGAGACTTAATCCTCTTCGGCAATAAATTGCGACCAGACCAAACCGTTGATGAGGGTACTGTCCGt                                            >  1:559700/2‑151 (MQ=255)

                                                                                                     cGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAAccc                                      >  2:260996/1‑151 (MQ=255)

                                                                                                        aaTCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGc                                    >  1:278744/1‑150 (MQ=255)

                                                                                                              ttttACTTGTTCACGCTTCTGTCCATCCTCAGCGCATGGCGTGATATAGTCGACGCTCCATTCATCCATGAGATACTTATTCCTCTTCTACAATAAAATGAGACCAGACCCCACAGTAGATGTGGTTGCTGACCGTAAACCCGCATa                                 <  1:705838/147‑1 (MQ=255)

                                                                                                              tttGACTTGTTCCCTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCCTTCAGCCATGAGATACTTAATCCTCTTCGTCACTAGATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCCTAgct                              >  1:779887/1‑148 (MQ=255)

                                                                                                                   cTTGTTCACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGt                        >  2:443125/1‑151 (MQ=255)

                                                                                                                         cACTCTTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGc                  >  2:245793/1‑151 (MQ=255)

                                                                                                                           ctctTCTTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGt                >  1:783506/1‑151 (MQ=255)

                                                                                                                            tcttctTGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGtt               >  2:672326/1‑151 (MQ=255)

                                                                                                                                cttGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCg           >  1:490788/1‑151 (MQ=255)

                                                                                                                                  tGCCGTGCTCAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTg         >  1:154386/1‑151 (MQ=255)

                                                                                                                                        gCTCAGCGCGTGGGCGGATATATTCGCTGCTACATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCg   >  1:398939/1‑151 (MQ=255)

                                                                                                                                           cAGCGCATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCgt  <  2:637982/149‑1 (MQ=255)

TCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGT  >  NC_000913/4128217‑4128504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A