Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,508,245	T→C	T116A (ACG→GCG)	php ←	phosphotriesterase homology protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,508,245	0	T	C	100.0%	172.3 / NA	49	T116A (ACG→GCG)	php	phosphotriesterase homology protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/31); total (18/31)

GCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCA  >  NC_000913/3508119‑3508390

gCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAAttt                                                                                                                            <  2:777187/150‑1 (MQ=255)

  gTGGAGATCAGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCAt                                                                                                                         >  1:431581/1‑151 (MQ=255)

    ggAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCg                                                                                                                       <  2:129699/151‑1 (MQ=255)

      agaTCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGAc                                                                                                                     <  1:304629/151‑1 (MQ=255)

        aTCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGAcc                                                                                                                    >  1:211563/1‑150 (MQ=255)

        aTCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCa                                                                                                                   <  2:593839/151‑1 (MQ=255)

           gggCGTCCGGTCTGGTTATGCGCCAGCGCCGCGGCAATAAATCCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGTTCTCCGCTATGATCCCGGCTTTCAGCTACGCGCCATCGATACCCTGTTCAATTTCATCGACCAtct                                                                                                                <  1:647848/151‑1 (MQ=255)

           gggCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCAtct                                                                                                                >  1:629741/1‑151 (MQ=255)

                 ccGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTgc                                                                                                           <  2:567512/150‑1 (MQ=255)

                  cGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTgcgc                                                                                                         <  2:394639/151‑1 (MQ=255)

                    gTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGcc                                                                                                        >  2:545070/1‑150 (MQ=255)

                    gTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCa                                                                                                       <  1:682417/151‑1 (MQ=255)

                              acgcCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCAc                                                                                             <  2:239966/150‑1 (MQ=255)

                                 ccAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCt                                                                                          <  2:306374/151‑1 (MQ=255)

                                  cagcgcagcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTg                                                                                         <  2:150910/151‑1 (MQ=255)

                                   agcgcagcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGc                                                                                        >  1:487265/1‑151 (MQ=255)

                                       cagcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGt                                                                                    <  1:695073/151‑1 (MQ=255)

                                       cagcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGt                                                                                    <  1:770973/151‑1 (MQ=255)

                                        agcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTc                                                                                   <  1:467564/151‑1 (MQ=255)

                                        agcgGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTc                                                                                   <  1:545033/151‑1 (MQ=255)

                                           ggCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCAAGTTCCTGCACGCTGCGGGTCg                                                                                  <  1:729259/149‑1 (MQ=255)

                                             cAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCcac                                                                              >  1:200581/1‑151 (MQ=255)

                                                     aCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTcc                                                                       <  1:497752/150‑1 (MQ=255)

                                                        ttCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCggg                                                                    >  2:726767/1‑150 (MQ=255)

                                                            ctTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGaaa                                                                 <  2:506635/149‑1 (MQ=255)

                                                               ccAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAAcg                                                             <  2:115836/150‑1 (MQ=255)

                                                                 aGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGt                                                          >  1:158513/1‑151 (MQ=255)

                                                                 aGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGt                                                          >  1:310732/1‑151 (MQ=255)

                                                                   cggcgTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTcc                                                        >  2:837597/1‑151 (MQ=255)

                                                                    ggcgTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCt                                                       <  2:848476/151‑1 (MQ=255)

                                                                    ggcgTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCt                                                       >  2:431359/1‑151 (MQ=255)

                                                                      cgTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTgg                                                     >  2:739434/1‑151 (MQ=255)

                                                                              ttCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAAc                                              <  2:653783/150‑1 (MQ=255)

                                                                               tCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGtaataa                                               <  1:17227/148‑1 (MQ=255)

                                                                                ccTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCg                                            <  2:30022/150‑1 (MQ=255)

                                                                                   tCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTAc                                        >  1:247482/1‑151 (MQ=255)

                                                                                    cGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACa                                       <  1:242387/151‑1 (MQ=255)

                                                                                           gCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGccac                                 <  1:557713/150‑1 (MQ=255)

                                                                                            ccGATCTCCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGccacc                                >  1:204699/1‑150 (MQ=255)

                                                                                                  tcCGCGATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTg                          >  2:169894/1‑150 (MQ=255)

                                                                                                    cgcgATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATc                       <  1:275911/151‑1 (MQ=255)

                                                                                                      cgATGATCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATccc                     <  1:789384/151‑1 (MQ=255)

                                                                                                           atCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGtct                >  1:183312/1‑151 (MQ=255)

                                                                                                           atCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGtct                <  1:629593/151‑1 (MQ=255)

                                                                                                            tCCCGGCTTTCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGtctc               >  2:517276/1‑151 (MQ=255)

                                                                                                                     tCAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTa       <  1:111487/150‑1 (MQ=255)

                                                                                                                      cAGCTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCATGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTAc      <  2:377427/150‑1 (MQ=255)

                                                                                                                         cTCCGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACa     <  1:851314/148‑1 (MQ=255)

                                                                                                                           ccGCGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCa  >  2:473512/1‑149 (MQ=255)

GCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCA  >  NC_000913/3508119‑3508390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A