Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F50 I3 R1 9 107.1 3412234 99.5% 3395172 150.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,214,770 A→C E575A (GAA→GCA)  rpoD → RNA polymerase, sigma 70 (sigma D) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,214,7700AC100.0% 260.8 / NA 64E575A (GAA→GCA) rpoDRNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (45/19);  total (45/19)

CACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCG  >  NC_000913/3214624‑3214916
                                                                                                                                                  |                                                                                                                                                  
caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTg                                                                                                                                                <  1:1290454/151‑1 (MQ=255)
caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTg                                                                                                                                                >  2:1489412/1‑151 (MQ=255)
caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTg                                                                                                                                                >  1:876609/1‑151 (MQ=255)
   ccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGt                                                                                                                                             >  2:137499/1‑151 (MQ=255)
      cGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTaaa                                                                                                                                          <  1:1406311/151‑1 (MQ=255)
            gCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTc                                                                                                                                    <  2:1632674/151‑1 (MQ=255)
             ccGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCg                                                                                                                                   >  1:599924/1‑151 (MQ=255)
               gCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCAGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGAc                                                                                                                                 >  1:802233/1‑151 (MQ=255)
                 tGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGt                                                                                                                               >  2:319771/1‑151 (MQ=255)
                        tGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCcgc                                                                                                                        >  1:543538/1‑151 (MQ=255)
                             ccaccGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAAc                                                                                                                    <  1:1701737/150‑1 (MQ=255)
                              caccGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGt                                                                                                                  >  1:545659/1‑151 (MQ=255)
                                 cGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATc                                                                                                               >  2:1283/1‑151 (MQ=255)
                                       ccTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAg                                                                                                         <  2:1393121/151‑1 (MQ=255)
                                        cTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTc                                                                                                           >  2:685154/1‑148 (MQ=255)
                                         tgcgtgcgGCAACGCACGACGTGCTGGATGGCCTGACCGCGCGTGAAGCAAAAGTTCTGAGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTc                                                                                                           >  2:813042/1‑147 (MQ=255)
                                            gtgcgGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATcg                                                                                                     >  2:1685909/1‑150 (MQ=255)
                                                  cAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATcgaagcgaa                                                                                              <  2:635472/151‑1 (MQ=255)
                                                     cGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGgc                                                                                           <  2:1083986/151‑1 (MQ=255)
                                                          gacgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTgc                                                                                      <  2:610417/151‑1 (MQ=255)
                                                            cgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTgcgc                                                                                    <  2:721621/151‑1 (MQ=255)
                                                               gctggctgGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCaa                                                                                  >  2:769142/1‑150 (MQ=255)
                                                                 tggctggcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACt                                                                               >  2:1420536/1‑151 (MQ=255)
                                                                 tggctggcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACt                                                                               >  2:1393240/1‑151 (MQ=255)
                                                                  ggctggcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACt                                                                               <  1:280320/150‑1 (MQ=255)
                                                                          tGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCAccc                                                                      >  1:611747/1‑151 (MQ=255)
                                                                             ccGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCg                                                                     >  2:625810/1‑149 (MQ=255)
                                                                                  cgTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGtt                                                              >  2:1353329/1‑151 (MQ=255)
                                                                                      aaGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTg                                                           >  2:226525/1‑150 (MQ=255)
                                                                                         cAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGt                                                       <  1:1477133/151‑1 (MQ=255)
                                                                                          aaaaGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGtg                                                      >  1:398136/1‑151 (MQ=255)
                                                                                            aaGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGtgct                                                    >  2:1455541/1‑151 (MQ=255)
                                                                                              gTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGGCGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCTCCCGAGCCGTTCTGAAGtgctgc                                                  <  1:54235/151‑1 (MQ=255)
                                                                                              gTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGtgctgc                                                  <  1:448434/151‑1 (MQ=255)
                                                                                              gTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGtgctgc                                                  >  2:234273/1‑151 (MQ=255)
                                                                                                tCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCg                                                 >  1:77648/1‑150 (MQ=255)
                                                                                                tCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGt                                                >  2:536004/1‑151 (MQ=255)
                                                                                                     gTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCtt                                           >  1:1007772/1‑151 (MQ=255)
                                                                                                      tATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTc                                          >  1:238072/1‑151 (MQ=255)
                                                                                                        tGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCt                                        >  1:1692027/1‑151 (MQ=255)
                                                                                                        tGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCt                                        >  2:1647763/1‑151 (MQ=255)
                                                                                                         gCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTg                                       <  1:224550/151‑1 (MQ=255)
                                                                                                          cGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTgg                                      <  2:375646/151‑1 (MQ=255)
                                                                                                           gTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCt                                        <  1:1662752/148‑1 (MQ=255)
                                                                                                           gTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTgg                                      >  2:904978/1‑150 (MQ=255)
                                                                                                           gTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGa                                     <  2:532196/151‑1 (MQ=255)
                                                                                                            tttCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGAc                                    >  2:802602/1‑151 (MQ=255)
                                                                                                             ttCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACg                                   >  1:1118746/1‑151 (MQ=255)
                                                                                                                   aTCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAAt                             >  1:1548532/1‑151 (MQ=255)
                                                                                                                     cGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCg                           >  2:536007/1‑151 (MQ=255)
                                                                                                                      gATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCgg                          >  2:1048050/1‑151 (MQ=255)
                                                                                                                        tatGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGt                         >  2:432604/1‑150 (MQ=255)
                                                                                                                        tatGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGt                         >  1:305370/1‑150 (MQ=255)
                                                                                                                              cacCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCgg                  >  2:1521611/1‑151 (MQ=255)
                                                                                                                              cacCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCgg                  >  2:1252829/1‑151 (MQ=255)
                                                                                                                               acCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCgg                  >  1:74359/1‑150 (MQ=255)
                                                                                                                                ccGACTACACGCTGGAAGCAGTGGGTAAACAGTTTGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGAt                >  1:86303/1‑151 (MQ=255)
                                                                                                                                ccGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGAt                <  2:709249/151‑1 (MQ=255)
                                                                                                                                 cGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATc               >  2:1013658/1‑151 (MQ=255)
                                                                                                                                 cGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATc               <  2:1617339/151‑1 (MQ=255)
                                                                                                                                 cGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATc               >  2:1186795/1‑151 (MQ=255)
                                                                                                                                  gACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATc               >  2:231378/1‑150 (MQ=255)
                                                                                                                                    cTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAgg            >  2:454310/1‑151 (MQ=255)
                                                                                                                                      acacGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCg          >  1:1129260/1‑151 (MQ=255)
                                                                                                                                              gaagcaGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTAcgccg  >  1:521712/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCG  >  NC_000913/3214624‑3214916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A