Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F50 I2 R1 173 150.8 4954114 96.5% 4780720 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,945,827 G→A H50H (CAC→CAT)  ruvA ← component of RuvABC resolvasome, regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,945,8270GA99.3% 587.1 / ‑5.5 150H50H (CAC→CAT) ruvAcomponent of RuvABC resolvasome, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (79/70);  total (79/71)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 151 total aligned reads displayed.

TAACGAACTGCTGCGCTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATC  >  NC_000913/1945679‑1945974
                                                                                                                                                    |                                                                                                                                                   
tAACGAACTGCTGCGCTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATg                                                                                                                                                   >  1:1414320/1‑151 (MQ=255)
 aaCGAACTGCTGCGCTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATgg                                                                                                                                                  <  4:1654368/151‑1 (MQ=255)
  aCGAACTGCTGCGCTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATggg                                                                                                                                                 <  1:1544945/151‑1 (MQ=255)
   cGAACTGCTGCGCTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATggg                                                                                                                                                 >  4:1313675/1‑150 (MQ=255)
   cGAACTGCTGCGCTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGt                                                                                                                                                <  1:1294439/151‑1 (MQ=255)
         gctgcGCTGACATTCCGGAGAAGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGaaa                                                                                                                                            <  4:522726/149‑1 (MQ=255)
          ctgcGCTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAAc                                                                                                                                          <  4:592466/150‑1 (MQ=255)
            gcgcTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACg                                                                                                                                         <  3:160268/149‑1 (MQ=255)
            gcgcTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGa                                                                                                                                        <  2:364029/150‑1 (MQ=255)
            gcgcTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGa                                                                                                                                        <  1:550057/150‑1 (MQ=255)
             cgcTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATc                                                                                                                                      >  4:1086923/1‑151 (MQ=255)
             cgcTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATc                                                                                                                                      <  1:37953/151‑1 (MQ=255)
             cgcTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATc                                                                                                                                      <  4:1132885/151‑1 (MQ=255)
              gcTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCg                                                                                                                                     <  4:1179386/151‑1 (MQ=255)
              gcTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCg                                                                                                                                     >  1:1772142/1‑151 (MQ=255)
                 gACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCt                                                                                                                                   <  1:578868/150‑1 (MQ=255)
                   cATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTcc                                                                                                                                >  1:1122722/1‑151 (MQ=255)
                    aTTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCt                                                                                                                               >  4:446446/1‑151 (MQ=255)
                       ccGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGAc                                                                                                                            <  3:368337/151‑1 (MQ=255)
                        cGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGAcc                                                                                                                           >  1:67570/1‑151 (MQ=255)
                        cGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGAcc                                                                                                                           >  1:1434291/1‑151 (MQ=255)
                          gagaGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCg                                                                                                                         <  2:261709/151‑1 (MQ=255)
                             agGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCtt                                                                                                                      <  2:166728/151‑1 (MQ=255)
                              ggATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTc                                                                                                                     <  3:44257/151‑1 (MQ=255)
                              ggATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTc                                                                                                                     >  1:1009708/1‑151 (MQ=255)
                                aTCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTc                                                                                                                     >  4:74551/1‑149 (MQ=255)
                                aTCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAg                                                                                                                   <  4:651063/151‑1 (MQ=255)
                                 tCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAgg                                                                                                                  >  4:1313335/1‑151 (MQ=255)
                                  cGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAggg                                                                                                                 >  4:1031163/1‑151 (MQ=255)
                                   gCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGa                                                                                                                <  3:463026/151‑1 (MQ=255)
                                    ccAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAggg                                                                                                                 >  2:485617/1‑149 (MQ=255)
                                    ccAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAg                                                                                                               <  2:351241/151‑1 (MQ=255)
                                     cAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGt                                                                                                              <  3:209160/151‑1 (MQ=255)
                                      aGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGtt                                                                                                             <  2:141190/151‑1 (MQ=255)
                                      aGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGtt                                                                                                             <  4:777125/151‑1 (MQ=255)
                                        cgcCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTc                                                                                                            >  1:1411341/1‑150 (MQ=255)
                                        cgcCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCa                                                                                                           <  1:514850/151‑1 (MQ=255)
                                         gcCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCAt                                                                                                          <  4:1640586/151‑1 (MQ=255)
                                         gcCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCAt                                                                                                          >  4:1873786/1‑151 (MQ=255)
                                         gcCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCAt                                                                                                          <  4:381801/151‑1 (MQ=255)
                                           cAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATaa                                                                                                        <  4:1070660/151‑1 (MQ=255)
                                           cAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATaa                                                                                                        <  3:519706/151‑1 (MQ=255)
                                           cAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATa                                                                                                         >  4:332890/1‑150 (MQ=255)
                                            aaCTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATaaa                                                                                                       <  1:63220/151‑1 (MQ=255)
                                               ttCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAAc                                                                                                    >  4:1494387/1‑151 (MQ=255)
                                                tCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATaaaaa                                                                                                     <  4:1773869/149‑1 (MQ=255)
                                                        aCGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCg                                                                                           <  4:911561/151‑1 (MQ=255)
                                                           ccGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGc                                                                                         >  1:1093356/1‑150 (MQ=255)
                                                               tGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCatat                                                                                     <  1:1086457/150‑1 (MQ=255)
                                                                ggTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGc                                                                                   <  4:1263796/151‑1 (MQ=255)
                                                                ggTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGc                                                                                   >  1:1463743/1‑151 (MQ=255)
                                                                 gTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGc                                                                                   >  4:615279/1‑150 (MQ=255)
                                                                 gTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCa                                                                                  <  4:1650728/151‑1 (MQ=255)
                                                                  ttttGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCAc                                                                                 >  1:1794206/1‑151 (MQ=255)
                                                                    ttGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACtt                                                                               >  4:694018/1‑151 (MQ=255)
                                                                       aTCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCAt                                                                            >  4:1183704/1‑151 (MQ=255)
                                                                        tCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCAt                                                                            >  4:1803684/1‑150 (MQ=255)
                                                                          aaCTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGc                                                                         >  1:1189667/1‑151 (MQ=255)
                                                                          aaCTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGc                                                                         <  1:9816/151‑1 (MQ=255)
                                                                                ttGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTa                                                                      <  1:81023/148‑1 (MQ=255)
                                                                                  gAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTAcgccg                                                                 >  4:907393/1‑151 (MQ=255)
                                                                                  gAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTAcgcc                                                                  <  3:226143/150‑1 (MQ=255)
                                                                                   aaCAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTAcgccgc                                                                >  1:253746/1‑151 (MQ=255)
                                                                                   aaCAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTAcgccgc                                                                >  3:341706/1‑151 (MQ=255)
                                                                                     cAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGccc                                                              >  4:410055/1‑151 (MQ=255)
                                                                                          tgCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTc                                                         >  4:1133345/1‑151 (MQ=255)
                                                                                            cgcTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCaa                                                       <  1:589200/151‑1 (MQ=255)
                                                                                             gcTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCAGAGCCTACGCCGCCCACTTCAAt                                                      <  1:1857921/151‑1 (MQ=255)
                                                                                               tctTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAAtt                                                     >  1:354208/1‑150 (MQ=255)
                                                                                                 ttGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAac                                                  >  2:259899/1‑151 (MQ=255)
                                                                                                 ttGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAac                                                  >  2:398174/1‑151 (MQ=255)
                                                                                                   gTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAaca                                                 <  4:1794910/150‑1 (MQ=255)
                                                                                                    tttATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACAcc                                               >  4:126202/1‑151 (MQ=255)
                                                                                                    tttATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACAcc                                               >  3:20159/1‑151 (MQ=255)
                                                                                                      tATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACAcc                                               >  4:1408190/1‑149 (MQ=255)
                                                                                                       aTTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGc                                            <  4:928368/151‑1 (MQ=255)
                                                                                                       aTTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGc                                            >  1:1244148/1‑151 (MQ=255)
                                                                                                       aTTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGc                                            >  4:1760634/1‑151 (MQ=255)
                                                                                                         tGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCg                                           <  4:409245/150‑1 (MQ=255)
                                                                                                         tGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCg                                           <  2:142112/150‑1 (MQ=255)
                                                                                                          gTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCggg                                         >  2:254526/1‑151 (MQ=255)
                                                                                                           ttAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCgggg                                        >  2:105614/1‑151 (MQ=255)
                                                                                                            tAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCggggg                                       >  4:1738563/1‑151 (MQ=255)
                                                                                                            tAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCgggg                                        <  1:270528/150‑1 (MQ=255)
                                                                                                             aaaaCCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGgt                                      >  2:437213/1‑151 (MQ=255)
                                                                                                              aaaCCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGgtt                                     >  1:47087/1‑151 (MQ=255)
                                                                                                              aaaCCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGgtt                                     <  1:518050/151‑1 (MQ=255)
                                                                                                                     aCAGCAGTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGttttt                               <  1:753055/150‑1 (MQ=255)
                                                                                                                      cagcagTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTc                              >  1:1313417/1‑150 (MQ=255)
                                                                                                                       agcagTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCa                             >  2:324984/1‑150 (MQ=255)
                                                                                                                         cagTTGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAatg                          <  1:16831/151‑1 (MQ=255)
                                                                                                                             tGCGCGTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAatgatg                       <  1:1849524/150‑1 (MQ=255)
                                                                                                                                  gTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGcc                  >  1:1298489/1‑150 (MQ=255)
                                                                                                                                  gTCTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCct                 <  3:32510/151‑1 (MQ=255)
                                                                                                                                    cTTCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCctct               >  3:101047/1‑151 (MQ=255)
                                                                                                                                      tCACGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCctc                <  4:1906171/148‑1 (MQ=255)
                                                                                                                                         cGCACCACAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTc          >  4:1789992/1‑151 (MQ=255)
                                                                                                                                             ccacAAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGcc      >  2:151666/1‑151 (MQ=255)
                                                                                                                                              cacaAAATGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCt     >  1:1367388/1‑151 (MQ=255)
                                                                                                                                                 aaaaTGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATc  <  4:156232/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TAACGAACTGCTGCGCTGACATTCCGGAGAGGATCGCCAGCGCCAACTTCGGGCCGACGCCGTTGGTTTTGATCAACTCTTTGAACAATGTGCGCTCTTGTTTATTGTTAAAACCGTACAGCAGTTGCGCGTCTTCACGCACCACAAAGTGGGTGAAAACGATCGCTTCCTGACCCGCTTCAGGGAGTTCATAAAAACAGGTCATCGGCATATGCACTTCATAGCCTACGCCGCCCACTTCAATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATC  >  NC_000913/1945679‑1945974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A