breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 34,876 A→G C166R (TGT→CGT)  caiE ← stimulator of CaiD and CaiB enzyme activities
RA 44,310 A→G N44S (AAC→AGC)  fixC → putative oxidoreductase
RA 48,048 A→G M94V (ATG→GTG)  kefC → potassium:proton antiporter
RA 96,645 T→C V215A (GTG→GCG)  mraY → phospho‑N‑acetylmuramoyl‑pentapeptide transferase
RA 126,862 C→T P390S (CCG→TCG)  aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 143,721 T→C V7A (GTG→GCG)  yadH → putative ABC transporter permease
RA 218,942 A→G V218A (GTT→GCT)  tsaA ← tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
RA 231,803 C→T P228S (CCG→TCG)  yafD → endo/exonuclease/phosphatase family protein
RA 246,784 G→A A25T (GCC→ACC)  yafL → putative lipoprotein and C40 family peptidase
MC JC 257,908 Δ776 bp [crl] [crl]
RA 261,402 G→A A339T (GCC→ACC)  proB → gamma‑glutamate kinase
RA 267,775 A→G R77R (CGT→CGC yafZ ← CP4‑6 prophage; conserved protein
RA 277,958 A→G L282P (CTC→CCC)  afuC ← CP4‑6 prophage; putative ferric transporter subunit
RA 277,996 A→G G269G (GGT→GGC afuC ← CP4‑6 prophage; putative ferric transporter subunit
RA 280,171 G→A pseudogene (192/249 nt) insX ← pseudogene, IS3 family transposase family
RA 295,230 G→A T117M (ACG→ATG)  yagN ← CP4‑6 prophage; uncharacterized protein
RA 313,726 A→G pseudogene (80/90 nt) ykgP ← pseudogene, oxidoreductase family
RA 378,025 T→C Y115C (TAC→TGC)  frmB ← S‑formylglutathione hydrolase
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein
RA 394,030 A→G pseudogene (1061/1449 nt) yaiT → pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
RA 450,945 A→G A222A (GCT→GCC cyoA ← cytochrome o ubiquinol oxidase subunit II
RA 483,580 T→C Y275C (TAC→TGC)  acrB ← multidrug efflux system protein
RA 483,985 A→G V140A (GTC→GCC)  acrB ← multidrug efflux system protein
RA 515,571 A→G I68T (ATC→ACC)  qmcA ← PHB domain membrane‑anchored putative protease
RA 523,741 (G)6→7 coding (481/4281 nt) rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 535,299 T→C F462L (TTC→CTC)  gcl → glyoxylate carboligase
RA 553,403 C→T V180I (GTC→ATC)  lpxH ← UDP‑2,3‑diacylglucosamine pyrophosphohydrolase
RA 574,383 G→A intergenic (+44/+146) quuD → / ← nmpC DLP12 prophage; putative antitermination protein/DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage
RA 577,085 G→A intergenic (‑260/‑313) nmpC ← / → essD DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage/DLP12 prophage; putative phage lysis protein
RA 578,102 C→T Q164* (CAA→TAA)  rrrD → DLP12 prophage; putative lysozyme
RA 586,073 C→T pseudogene (17/45 nt) pauD → tRNA‑OTHER
RA 598,492 A→G E338E (GAA→GAG cusB → copper/silver efflux system, membrane fusion protein
RA 620,791 A→G V133A (GTC→GCC)  fepG ← iron‑enterobactin ABC transporter permease
RA 642,536 A→G N150S (AAT→AGT)  ybdR → putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 701,574 A→G A7A (GCT→GCC nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
RA 706,673 T→C I194T (ATT→ACT)  glnS → glutamyl‑tRNA synthetase
RA 723,576 A→G V280A (GTC→GCC)  kdpD ← fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein
RA 741,429 A→G S376S (TCT→TCC dtpD ← dipeptide and tripeptide permease D
RA 747,436 T→C T112A (ACC→GCC)  abrB ← regulator of aidB expression; inner membrane protein
RA 771,344 T→C intergenic (+733/‑114) mngB → / → cydA alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
RA 780,999 A→G intergenic (+81/‑69) lysW → / → valZ tRNA‑Lys/tRNA‑Val
RA 781,084 T→C noncoding (17/76 nt) valZ → tRNA‑Val
RA 785,828 C→T P66S (CCT→TCT)  aroG → 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, phenylalanine repressible
RA 797,155 T→C V201A (GTA→GCA)  modC → molybdate ABC transporter ATPase; chlorate resistance protein
RA 807,115 T→C Q56R (CAA→CGA)  ybhC ← acyl‑CoA thioesterase, lipoprotein
RA 868,415 C→T A299V (GCC→GTC)  gsiA → glutathione ABC transporter ATPase
RA 897,997 C→T D3D (GAC→GAT ybjO → DUF2593 family inner membrane protein
RA 925,555 A→G intergenic (+15/+329) clpA → / ← serW ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity/tRNA‑Ser
RA 929,067 G→A L43L (CTC→CTT cydC ← glutathione/cysteine ABC transporter export permease/ATPase
JC JC 944,912 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp intergenic (+16/+14) dmsC → / ← ycaC dimethyl sulfoxide reductase, anaerobic, subunit C/putative isochorismatase family hydrolase
RA 964,634 T→C R105R (CGT→CGC ycaI → ComEC family inner membrane protein
RA 980,655 A→G T1444A (ACC→GCC)  mukB → chromosome condensin MukBEF, ATPase and DNA‑binding subunit
RA 1,009,102 T→C L420P (CTG→CCG)  rlmL → 23S rRNA m(2)G2445 and m(7)G2069 methyltransferases, SAM‑dependent
RA 1,097,218 (G)7→8 intergenic (+389/+347) ycdU → / ← serX putative inner membrane protein/tRNA‑Ser
RA 1,154,782 A→G E224G (GAA→GGA)  yceG → septation protein, ampicillin sensitivity
RA 1,158,225 C→T L119L (CTC→CTT ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
RA 1,169,759 C→T D206N (GAT→AAT)  ldtC ← L,D‑transpeptidase linking Lpp to murein
RA 1,201,206 T→C intergenic (‑174/‑291) xisE ← / → ymfI e14 prophage; putative excisionase/e14 prophage; uncharacterized protein
RA 1,205,497 T→C pseudogene (1094/1134 nt) aaaE → pseudogene, phage terminase protein A family, e14 prophage;Phage or Prophage Related
RA 1,226,899 T→C G100G (GGT→GGC ycgJ → uncharacterized protein
RA 1,246,015 T→C A454A (GCA→GCG treA ← periplasmic trehalase
RA 1,308,170 T→C D159G (GAC→GGC)  clsA ← cardiolipin synthase 1
RA 1,308,467 T→C E60G (GAG→GGG)  clsA ← cardiolipin synthase 1
RA 1,314,744 T→C T161A (ACT→GCT)  yciE ← putative rubrerythrin/ferritin‑like metal‑binding protein
RA 1,345,482 G→A Q421* (CAG→TAG)  gmr ← cyclic‑di‑GMP phosphodiesterase; csgD regulator; modulator of RNase II stability
RA 1,348,601 T→C P104P (CCA→CCG rnb ← ribonuclease II
RA 1,370,155 A→G intergenic (+152/‑61) pspE → / → ycjM thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein
RA 1,388,699 A→G V38A (GTC→GCC)  tpx ← lipid hydroperoxide peroxidase
RA 1,411,458 T→C Y164C (TAC→TGC)  ttcA ← tRNA s(2)C32 thioltransferase, iron sulfur cluster protein
RA 1,420,637 C→T N91N (AAC→AAT ydaS → Rac prophage; putative DNA‑binding transcriptional regulator
RA 1,423,878 T→C F33L (TTT→CTT)  trkG → Rac prophage; potassium transporter subunit
RA 1,432,646 T→G V79G (GTG→GGG)  tfaR → Rac prophage; putative tail fiber assembly protein
RA 1,436,536 A→G Y120H (TAC→CAC)  ompN ← outer membrane pore protein N, non‑specific
RA 1,457,427 A→G Y333C (TAT→TGT)  paaE → ring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component
RA 1,556,571 T→C intergenic (‑291/‑54) bdm ← / → osmC biofilm‑dependent modulation protein/lipoyl‑dependent Cys‑based peroxidase, hydroperoxide resistance; salt‑shock inducible membrane protein; peroxiredoxin
RA 1,644,942 C→T intergenic (‑40/+177) rem ← / ← hokD Qin prophage; uncharacterized protein/Qin prophage; small toxic polypeptide
RA 1,740,586 T→C S226P (TCA→CCA)  ydhC → putative arabinose efflux transporter
RA 1,772,863 C→T L118L (CTG→TTG)  ydiN → putative MFS transporter, membrane protein
RA 1,863,313 T→C A181A (GCT→GCC gapA → glyceraldehyde‑3‑phosphate dehydrogenase A
RA 1,883,282 T→C L32P (CTC→CCC)  yeaV → putative transporter
RA 1,945,827 G→A H50H (CAC→CAT ruvA ← component of RuvABC resolvasome, regulatory subunit
RA 1,954,771 A→G V746A (GTG→GCG)  torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit
RA 1,955,440 T→C Q523R (CAG→CGG)  torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit
RA 1,961,199 T→C F380L (TTC→CTC)  argS → arginyl‑tRNA synthetase
RA 1,975,275 T→C D17G (GAC→GGC)  cheA ← fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,000,046 G→A R114C (CGC→TGC)  fliY ← cystine transporter subunit
RA 2,049,101 T→C G1388G (GGT→GGC yeeJ → putative adhesin
RA 2,098,260 G→A D14D (GAC→GAT wzzB ← regulator of length of O‑antigen component of lipopolysaccharide chains
RA 2,117,002 T→C T42T (ACA→ACG wcaL ← putative glycosyl transferase
RA 2,163,510 T→C V212A (GTA→GCA)  baeS → sensory histidine kinase in two‑component regulatory system with BaeR
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,202,588 T→C A770A (GCT→GCC yehI → DUF4132 domain‑containing protein
RA 2,224,908 C→T intergenic (‑38/+136) pbpG ← / ← yohC D‑alanyl‑D‑alanine endopeptidase/Yip1 family inner membrane protein
RA 2,241,409 T→C Q87R (CAG→CGG)  galS ← galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor
RA 2,244,134 A→G V75V (GTA→GTG yeiG → S‑formylglutathione hydrolase
RA 2,254,920 G→A G89G (GGC→GGT rihB ← ribonucleoside hydrolase 2
RA 2,258,247 A→G F41L (TTC→CTC)  psuG ← pseudouridine 5'‑phosphate glycosidase
RA 2,430,394 T→C T124A (ACC→GCC)  cvpA ← colicin V production protein
RA 2,441,814 T→C G17G (GGT→GGC mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
RA 2,442,524 C→T A254V (GCG→GTG)  mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
RA 2,449,635 (C)5→6 coding (415/822 nt) yfcO ← DUF2544 family putative outer membrane protein
RA 2,460,381 G→A T30M (ACG→ATG)  fadI ← beta‑ketoacyl‑CoA thiolase, anaerobic, subunit
RA 2,467,446 A→G K301R (AAG→AGG)  intS → CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase
RA 2,534,187 A→G E41G (GAA→GGA)  ptsI → PEP‑protein phosphotransferase of PTS system (enzyme I)
RA 2,539,372 T→C N71S (AAC→AGC)  cysM ← cysteine synthase B (O‑acetylserine sulfhydrolase B)
RA 2,589,108 A→G H505R (CAT→CGT)  acrD → aminoglycoside/multidrug efflux system
RA 2,609,458 T→C R324R (CGT→CGC hyfG → hydrogenase 4, subunit
RA 2,611,725 A→G H90R (CAC→CGC)  hyfJ → putative processing element hydrogenase 4
RA 2,629,930 A→G T47A (ACC→GCC)  yfgH → outer membrane integrity lipoprotein
MC JC 2,636,790 (TTC)3→2 coding (565‑567/1473 nt) der ← GTPase; multicopy suppressor of ftsJ
RA 2,647,150 G→A A59V (GCT→GTT)  pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
RA 2,662,523 A→G intergenic (‑392/+60) iscR ← / ← trmJ isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
RA 2,706,291 A→G R278R (CGT→CGC lepA ← back‑translocating elongation factor EF4, GTPase
RA 2,767,140 T→C L53L (TTG→CTG)  rnlB → CP4‑57 prophage; uncharacterized protein
RA 2,780,330 G→A G799G (GGC→GGT ypjA ← adhesin‑like autotransporter
RA 2,857,878 T→C R262R (CGT→CGC mutS → methyl‑directed mismatch repair protein
RA 2,865,819 T→C V240A (GTC→GCC)  ygbN → putative transporter
RA 2,889,256 T→C T282A (ACG→GCG)  cysI ← sulfite reductase, beta subunit, NAD(P)‑binding, heme‑binding
RA 2,912,885 A→G I256T (ATC→ACC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,960,844 T→C T529A (ACG→GCG)  recC ← exonuclease V (RecBCD complex), gamma chain
RA 2,978,727 T→C N58S (AAT→AGT)  lysA ← diaminopimelate decarboxylase, PLP‑binding
RA 2,994,896 G→C pseudogene (197/633 nt) ygeK ← pseudogene, response regulator family, part of T3SS PAI ETT2 remnant; putative 2‑component transcriptional regulator
RA 2,998,606 A→G pseudogene (223/1137 nt) ygeQ ← pseudogene, glycosyl hydrolase family 15, part of T3SS PAI ETT2 remnant
RA 3,018,435 A→G E792E (GAA→GAG ygfK → putative Fe‑S subunit oxidoreductase subunit
RA 3,071,369 T→C intergenic (‑125/+90) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,086,618 A→G intergenic (+216/‑88) yqgC → / → metK uncharacterized protein/S‑adenosylmethionine synthetase
RA 3,272,271 Δ1 bp coding (481/885 nt) garR ← tartronate semialdehyde reductase
RA 3,297,933 T→C Y69C (TAC→TGC)  yraQ ← putative inner membrane permease
RA 3,319,794 T→C R386R (CGT→CGC argG → argininosuccinate synthetase
RA 3,376,403 T→C D125G (GAC→GGC)  sspB ← ClpXP protease specificity enhancing factor
RA 3,377,215 A→G L69P (CTG→CCG)  sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
RA 3,440,116 T→C D305G (GAC→GGC)  rpoA ← RNA polymerase, alpha subunit
RA 3,449,100 T→C T24A (ACG→GCG)  rplP ← 50S ribosomal subunit protein L16
RA 3,453,207 T→C T22A (ACC→GCC)  rpsJ ← 30S ribosomal subunit protein S10
RA 3,507,100 T→C L79L (TTA→TTG yhfT ← inner membrane protein
RA 3,511,296 T→C T43A (ACG→GCG)  yhfY ← PRD domain protein
RA 3,515,565 A→G Y117H (TAC→CAC)  dam ← DNA adenine methyltransferase
RA 3,537,161 A→G Y117C (TAC→TGC)  greB → transcript cleavage factor
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,589,630 A→G V133A (GTG→GCG)  ugpE ← sn‑glycerol‑3‑phosphate ABC transporter permease
RA 3,602,371 C→T G126E (GGG→GAG)  ftsE ← cell division ATP‑binding protein
RA 3,612,540 T→C W226R (TGG→CGG)  yhhT → UPF0118 family putative transporter
RA 3,707,870 T→C intergenic (‑165/+746) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,778,196 C→T L266L (CTC→CTT lldP → L‑lactate permease
RA 3,815,808 (A)8→7 intergenic (‑40/+26) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,845,824 T→C E448E (GAA→GAG uhpT ← hexose phosphate transporter
RA 3,882,798 T→C D311G (GAC→GGC)  dnaA ← chromosomal replication initiator protein DnaA, DNA‑binding transcriptional dual regulator
RA 3,891,768 G→A A52T (GCA→ACA)  mdtL → multidrug efflux system protein
RA 3,937,749 G→A A152A (GCG→GCA rbsK → ribokinase
RA 3,948,964 C→T A336T (GCC→ACC)  yifB ← magnesium chelatase family protein and putative transcriptional regulator
RA 4,003,303 A→G R292R (CGT→CGC rarD ← putative chloramphenical resistance permease
RA 4,005,381 A→G S174G (AGC→GGC)  pldA → outer membrane phospholipase A
RA 4,076,509 A→G S122G (AGC→GGC)  yihY → BrkB family putative transporter, inner membrane protein
RA 4,078,459 T→C intergenic (+21/‑838) yiiD → / → yiiE GNAT family putative N‑acetyltransferase/CopG family putative transcriptional regulator
RA 4,118,516 T→C M1T (ATG→ACG) † zapB → FtsZ stabilizer, septal ring assembly factor, cell division stimulator
RA 4,128,316 A→G V27A (GTT→GCT)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding
RA 4,185,098 A→G Y1285C (TAC→TGC)  rpoB → RNA polymerase, beta subunit
RA 4,229,213 C→T L495L (CTG→TTG)  yjbB → putative Na+/Pi‑cotransporter
RA 4,264,246 G→A intergenic (‑15/‑68) qorA ← / → dnaB quinone oxidoreductase, NADPH‑dependent/replicative DNA helicase
MC JC 4,284,437 (CCA)4→3 coding (464‑466/1650 nt) actP ← acetate transporter
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,342,193 A→G T95A (ACG→GCG)  melA → alpha‑galactosidase, NAD(P)‑binding
RA 4,343,909 A→G M181V (ATG→GTG)  melB → melibiose:sodium symporter
RA 4,350,244 A→G V473V (GTT→GTC dcuS ← sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
RA 4,366,223 A→G F184S (TTC→TCC)  dcuA ← C4‑dicarboxylate antiporter
RA 4,399,151 Δ1 bp coding (1740/1848 nt) mutL → methyl‑directed mismatch repair protein
RA 4,493,603 T→C G268G (GGA→GGG idnD ← L‑idonate 5‑dehydrogenase, NAD‑binding
RA 4,500,977 A→G intergenic (‑186/‑283) yjgX ← / → yjgZ pseudogene fragment/uncharacterized protein
RA 4,550,731 A→G V189A (GTA→GCA)  gntP ← fructuronate transporter
RA 4,590,696 A→G V195A (GTC→GCC)  yjiY ← putative transporter
RA 4,613,359 C→T P317S (CCA→TCA)  yjjU → putative patatin‑like family phospholipase
RA 4,631,960 C→T P410S (CCG→TCG)  slt → lytic murein transglycosylase, soluble

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423706–3424234 3424589–3424238 5–884 93 [91] [91] 92 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =16 (0.100)90 (0.660) 71/266 2.1 79.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 33 (0.240)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780516 (0.100)93 (0.680) 77/266 1.5 79.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 33 (0.240)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)159 (1.060) 122/290 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein