Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	702,903	T→C	M237V (ATG→GTG)	nagB ←	glucosamine‑6‑phosphate deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	702,903	0	T	C	100.0%	119.9 / NA	32	M237V (ATG→GTG)	nagB	glucosamine‑6‑phosphate deaminase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/10); total (22/10)

ATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCCAGCAGTGTACCAA  >  NC_000913/702775‑703043

atTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTg                                                                                                                         >  2:169045/1‑150 (MQ=255)

 ttGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGc                                                                                                                        >  1:766337/1‑150 (MQ=255)

            aaGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATg                                                                                                            >  1:78855/1‑151 (MQ=255)

               gAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTc                                                                                                         <  2:560976/151‑1 (MQ=255)

                    agggcagggATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACat                                                                                                    <  1:703003/151‑1 (MQ=255)

                         agggATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGt                                                                                               >  2:141730/1‑151 (MQ=255)

                          gggATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTGTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGtt                                                                                              <  2:196324/151‑1 (MQ=255)

                             aTAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCAc                                                                                           >  1:523350/1‑151 (MQ=255)

                                  aaTTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAAc                                                                                      >  1:627918/1‑151 (MQ=255)

                                               tGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACggcggc                                                                         >  1:104032/1‑151 (MQ=255)

                                                gATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACggcggc                                                                         <  1:598786/150‑1 (MQ=255)

                                                gATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGcc                                                                        >  2:270080/1‑151 (MQ=255)

                                                  tatTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCt                                                                       <  2:282087/150‑1 (MQ=255)

                                                        cTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAgcgc                                                                >  2:247037/1‑151 (MQ=255)

                                                                    cATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTg                                                    >  1:293283/1‑151 (MQ=255)

                                                                      ttGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGc                                                  >  2:581821/1‑151 (MQ=255)

                                                                      ttGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGc                                                  >  1:715354/1‑151 (MQ=255)

                                                                         aaaTATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTAc                                               >  1:743355/1‑151 (MQ=255)

                                                                          aaTATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTAcc                                              >  1:389239/1‑151 (MQ=255)

                                                                                 tAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCAcc                                       >  2:303847/1‑151 (MQ=255)

                                                                                     gTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGa                                    >  1:103161/1‑150 (MQ=255)

                                                                                          aaCTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAatcatc                              >  1:567763/1‑151 (MQ=255)

                                                                                           aCTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAatcatc                              >  1:20629/1‑150 (MQ=255)

                                                                                             tttCAGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCAct                           >  1:212537/1‑151 (MQ=255)

                                                                                                 aGCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCActtctt                       <  2:235883/151‑1 (MQ=255)

                                                                                                  gCTCCATGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACttcttc                      <  1:311772/151‑1 (MQ=255)

                                                                                                       aTGGTGGAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCAt                 <  2:627881/151‑1 (MQ=255)

                                                                                                        tggtggAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCAAGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATc                >  2:505352/1‑151 (MQ=255)

                                                                                                          gtggAAGGTTCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCca              <  2:158588/151‑1 (MQ=255)

                                                                                                                  ttCATCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCCAGCAgtgt       >  1:40363/1‑150 (MQ=255)

                                                                                                                      tCGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCCAGCAGTGTACCa   >  2:183525/1‑150 (MQ=255)

                                                                                                                       cGCACACCACGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCCAGCAGTGTACCaa  <  2:104030/150‑1 (MQ=255)

ATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAATTACAGACCTTTGATATTTTCTGCTTCTAATTCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCCAGCAGTGTACCAA  >  NC_000913/702775‑703043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A