Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F50 I1 R1
|
163 |
55.0 |
1534755 |
98.6% |
1513268 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,279,364 |
A→G |
intergenic (+16/‑500) |
narK → / → narG |
nitrate/nitrite transporter/nitrate reductase 1, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,279,364 | 0 | A | G | 100.0%
| 67.7
/ NA
| 19 | intergenic (+16/‑500) | narK/narG | nitrate/nitrite transporter/nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (3/16); total (3/16) |
GGTAGCTCGCTGGCATTAACGGGTTCGCCAGTCGGCGCAATGAAGGTATTTTTGATTTTCTATATCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATACTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAA > NC_000913/1279232‑1279476
|
ggTAGCTCGCTGGCATTAACGGGTTCGCCAGTCGGCGCAATGAAGGTATTTTTGATTTTCTATATCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGtgct < 1:611122/151‑1 (MQ=255)
cGCCAGTCGGCGCAATGAAGGTATTTTTGATTTTCTATATCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGt < 1:540639/150‑1 (MQ=255)
cGGCGCAATGAAGGTATTTTTGATTTTCTATATCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCacttac < 2:593912/150‑1 (MQ=255)
gcgcAATGAAGGTATTTTTGATTTTCTATATCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACttt < 1:363491/151‑1 (MQ=255)
gcgcAATGAAGGTATTTTTGATTTTCTATATCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACttt < 1:695837/151‑1 (MQ=255)
ggTATTTTTGATTTTCTATATCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTcc < 1:391095/151‑1 (MQ=255)
tataTCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACtt > 1:694374/1‑151 (MQ=255)
tataTCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACtt > 2:432162/1‑151 (MQ=255)
tataTCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACtt > 2:477543/1‑151 (MQ=255)
atatCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTg < 1:432183/151‑1 (MQ=255)
atatCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTg < 2:694332/151‑1 (MQ=255)
atCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTgcc < 2:29549/151‑1 (MQ=255)
gCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTgccgcc < 1:724991/151‑1 (MQ=255)
gCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCtt < 1:725407/149‑1 (MQ=255)
gTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCAGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTg < 2:3150/151‑1 (MQ=255)
gATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAAc < 1:470904/151‑1 (MQ=255)
aCCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGaa < 1:532531/151‑1 (MQ=255)
gggCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATta < 1:586654/150‑1 (MQ=255)
atGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATGCTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTaa < 2:59200/151‑1 (MQ=255)
|
GGTAGCTCGCTGGCATTAACGGGTTCGCCAGTCGGCGCAATGAAGGTATTTTTGATTTTCTATATCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCATTCTAAAAAATAAACCGTTACTCGTCATACTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAA > NC_000913/1279232‑1279476
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
