Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,429,684	A→G	S212S (TCA→TCG)	stfR →	Rac prophage; putative tail fiber protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,429,684	0	A	G	100.0%	160.3 / NA	47	S212S (TCA→TCG)	stfR	Rac prophage; putative tail fiber protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/26); total (21/26)

GTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGC  >  NC_000913/1429537‑1429827

gtcgtcGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCg                                                                                                                                              <  2:90600/151‑1 (MQ=255)

                  gTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGcc                                                                                                                            >  1:319894/1‑151 (MQ=255)

                      tCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCca                                                                                                                           <  2:479619/148‑1 (MQ=255)

                       ccAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACAt                                                                                                                        <  2:511219/150‑1 (MQ=255)

                          gcgcAGGAACGGCATCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGc                                                                                                                    >  2:520299/1‑151 (MQ=255)

                           cgcAGGAACGGCATCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAGTCGCGTCGGGCGTCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCTTCACTACAATCAGCTGTCACATCTGCa                                                                                                                   <  2:716078/151‑1 (MQ=255)

                                  aCGGCATCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACcg                                                                                                            <  1:626502/151‑1 (MQ=255)

                                   cgccATCAGCAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGCGTCCTCAAAAAGCGCGGCGCCCACCAGTGCCGGTGCGGCGAAAACGTCAGAACCGAATGCTCCGGCGTCACTACAATCAGCAGCCCCATCTGCATCCAcc                                                                                                             >  2:420396/4‑149 (MQ=255)

                                       aTCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGAcc                                                                                                       >  2:477964/1‑151 (MQ=255)

                                        tCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGAcc                                                                                                       >  2:471187/1‑150 (MQ=255)

                                            caaAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGa                                                                                                   >  2:735633/1‑150 (MQ=255)

                                            caaAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGa                                                                                                   >  2:297644/1‑150 (MQ=255)

                                             aaaGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGa                                                                                                   >  1:444674/1‑149 (MQ=255)

                                               aGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGc                                                                                               <  2:100548/151‑1 (MQ=255)

                                                ggCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGc                                                                                               >  2:627789/1‑150 (MQ=255)

                                                 gCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCAt                                                                                             >  2:469215/1‑151 (MQ=255)

                                                  ccACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATc                                                                                            >  2:637574/1‑151 (MQ=255)

                                                    aCTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAg                                                                                          <  2:419600/151‑1 (MQ=255)

                                                               aaaaaGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGAcc                                                                               <  1:373937/151‑1 (MQ=255)

                                                                 aaaGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTc                                                                             >  1:200488/1‑151 (MQ=255)

                                                                  aaGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCg                                                                            <  1:71831/151‑1 (MQ=255)

                                                                  aaGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCg                                                                            <  2:469924/151‑1 (MQ=255)

                                                                          ccGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGa                                                                    <  2:301851/151‑1 (MQ=255)

                                                                                      ccTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTc                                                        <  1:164705/151‑1 (MQ=255)

                                                                                        tCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCa                                                       >  2:220149/1‑150 (MQ=255)

                                                                                         cAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCaaa                                                     <  2:761661/151‑1 (MQ=255)

                                                                                          aaaaaGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCaaa                                                     >  2:264424/1‑150 (MQ=255)

                                                                                           aaaaGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCaaa                                                     >  1:4113/1‑149 (MQ=255)

                                                                                                cgcgGGGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCCGCCTCAAaag                                                   >  2:239827/1‑146 (MQ=255)

                                                                                                      ggcCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCaaaa                                        <  2:683906/151‑1 (MQ=255)

                                                                                                             aGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAg                                 <  2:719052/151‑1 (MQ=255)

                                                                                                                gCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGaaa                              <  1:57593/151‑1 (MQ=255)

                                                                                                                       cggcgAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGc                       >  2:513012/1‑151 (MQ=255)

                                                                                                                        ggcgAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGca                      <  1:742998/151‑1 (MQ=255)

                                                                                                                           gAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGcatc                    >  2:30105/1‑150 (MQ=255)

                                                                                                                           gAAAACGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGcatc                    <  1:700719/150‑1 (MQ=255)

                                                                                                                            aaaaCGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGcatcat                  >  1:747977/1‑151 (MQ=255)

                                                                                                                               aCGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCaa               <  1:269208/151‑1 (MQ=255)

                                                                                                                                cGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAg              <  2:200480/151‑1 (MQ=255)

                                                                                                                                cGTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAg              <  2:368103/151‑1 (MQ=255)

                                                                                                                                 gTCAGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAgc             <  1:532486/151‑1 (MQ=255)

                                                                                                                                    aGAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGcc          >  1:65516/1‑151 (MQ=255)

                                                                                                                                     gAAACGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCa         <  1:71294/151‑1 (MQ=255)

                                                                                                                                       aaCGAATGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCagt       <  1:613460/151‑1 (MQ=255)

                                                                                                                                           aaTGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTg   <  2:194134/151‑1 (MQ=255)

                                                                                                                                            aTGCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGc  <  2:644512/151‑1 (MQ=255)

                                                                                                                                              gCTTCGGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGc  >  1:718427/1‑149 (MQ=255)

GTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGC  >  NC_000913/1429537‑1429827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A