Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,032,972	C→T	T36T (ACG→ACA)	yedJ ←	putative HD superfamily phosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,032,972	0	C	T	100.0%	186.1 / NA	50	T36T (ACG→ACA)	yedJ	putative HD superfamily phosphohydrolase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/33); total (17/33)

AGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCCGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACGCGCTCCGTTGCCAAAGAGTCGCCACCTTAGCAGT  >  NC_000913/2032829‑2033116

aGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGccc                                                                                                                                           >  1:533431/1‑151 (MQ=255)

         gTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACgcggc                                                                                                                                  <  1:451279/151‑1 (MQ=255)

           ctctTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACgcggcg                                                                                                                                 >  2:522909/1‑150 (MQ=255)

            tcttctGCCGCCAGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGa                                                                                                                                >  1:292502/1‑150 (MQ=255)

             cttctGCCGCCAGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACgcggcg                                                                                                                                 <  1:742907/148‑1 (MQ=255)

               tctGCCGCCAGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGaaa                                                                                                                              <  2:458660/149‑1 (MQ=255)

                    cgccAGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGacac                                                                                                                       >  2:651161/1‑151 (MQ=255)

                        aGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACg                                                                                                                    >  1:660426/1‑150 (MQ=255)

                        aGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGt                                                                                                                   <  1:320163/151‑1 (MQ=255)

                            ttGAAGAACGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGa                                                                                                                                        <  1:447391/126‑1 (MQ=255)

                            ttGAAGAACGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGa                                                                                                                                        >  2:447370/1‑126 (MQ=255)

                              gaagaaCGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATggg                                                                                                             <  2:368899/151‑1 (MQ=255)

                              gaagaaCGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATggg                                                                                                             <  2:432699/151‑1 (MQ=255)

                              gaagaaCGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATgg                                                                                                              <  1:653816/150‑1 (MQ=255)

                                  aaCGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCcgc                                                                                                         <  2:757050/151‑1 (MQ=255)

                                  aaCGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCcgc                                                                                                         <  1:348242/151‑1 (MQ=255)

                                        gCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCt                                                                                                    <  2:484732/150‑1 (MQ=255)

                                        gCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCt                                                                                                    <  2:576225/150‑1 (MQ=255)

                                              gCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCtg                                                                                             <  2:636740/151‑1 (MQ=255)

                                              gCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCt                                                                                              <  2:180614/150‑1 (MQ=255)

                                              gCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCt                                                                                              <  2:476458/150‑1 (MQ=255)

                                                 gatgatTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCtgatg                                                                                          <  2:50688/151‑1 (MQ=255)

                                                 gatgatTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCtgat                                                                                           >  2:727233/1‑150 (MQ=255)

                                                    gatTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCcg                                                                                                          <  1:355834/132‑1 (MQ=255)

                                                    gatTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCcg                                                                                                          >  2:355819/1‑132 (MQ=255)

                                                    gatTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGatgatg                                                                                       >  2:620316/1‑151 (MQ=255)

                                                         ttCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGttt                                                                                   <  1:667336/150‑1 (MQ=255)

                                                          tCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGtttt                                                                                  <  1:634241/150‑1 (MQ=255)

                                                                          atCGTGAAAATAACAGGCGGGTAAAATCACCAGAATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAAACGCCGCGAAAATGACACACGTCATGTGCCGCGTACTGGTGATGATGATGGATTTTCAACCAGTTTTCGAAc                                                                 >  2:762674/1‑151 (MQ=255)

                                                                            cGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACt                                                                <  2:388135/150‑1 (MQ=255)

                                                                                  aaTAACAGGCGGTTAAAATCACCAGCATATCGAAATCGTCATCTGCCGCCAGCTTCTGTGCTTTAGCCCAAACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGGGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGtgcc                                                           >  2:426072/1‑149 (MQ=255)

                                                                                     aaCAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGtgcctgcc                                                       >  1:283847/1‑150 (MQ=255)

                                                                                         ggCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAgtgt                                                  >  1:213397/1‑151 (MQ=255)

                                                                                         ggCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAgtgt                                                  >  1:119188/1‑151 (MQ=255)

                                                                                             tttAAAATCACCAGCATATCTACAGCGTCATCTGCCGCCAGCTTCTGTGCTTTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGCCCTGGTGCTGATGATGGTTTTGCACCCAGTTTACGAACTGTGCCTGCCAGtgttgt                                               <  2:462767/149‑1 (MQ=255)

                                                                                             gTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTa                                              <  1:134038/151‑1 (MQ=255)

                                                                                                 aaaTCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTc                                          <  2:241104/151‑1 (MQ=255)

                                                                                                      aCCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATAc                                     >  2:570914/1‑151 (MQ=255)

                                                                                                          gCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACgcgc                                 <  1:745389/151‑1 (MQ=255)

                                                                                                           cATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACGCGCt                                <  1:319399/151‑1 (MQ=255)

                                                                                                                      cGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACGCGCTCCGTTGCCaaa                     <  1:207808/151‑1 (MQ=255)

                                                                                                                      cGTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACGCGCTCCGTTGCCaa                      >  1:29340/1‑150 (MQ=255)

                                                                                                                       gTCATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCATTGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACGCGCTCCGTTGCCaaa                     <  2:561660/150‑1 (MQ=255)

                                                                                                                         cATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACGCGCTCCGTTGCCAAagag                  <  1:481431/151‑1 (MQ=255)

                                                                                                                         cATCTGCCGCCAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACGCGCTCCGTTGCCAAag                    >  2:183992/1‑149 (MQ=255)

                                                                                                                              gccgccAGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACGCGCTCCGTTGCCAAAGAGTCGc              <  2:638030/150‑1 (MQ=255)

                                                                                                                                    aGCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACGCGCTCCGTTGCCAAAGAGTCGCCACCTTa       <  2:169189/151‑1 (MQ=255)

                                                                                                                                     gCTTCTGTGCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACGCGCTCCGTTGCCAAAGAGTCGCCACCTTAg      <  1:694354/151‑1 (MQ=255)

                                                                                                                                           gtgCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACGCGCTCCGTTGCCAAAGAGTCGCCACCTTAGCAg   <  2:539562/148‑1 (MQ=255)

                                                                                                                                           gtgCTGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACGCGCTCCGTTGCCAAAGAGTCGCCACCTTAGCAGt  <  1:337381/149‑1 (MQ=255)

AGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTCGCCAGGCTCACAATATCGTGAAAATAACAGGCGGTTAAAATCACCAGCATATCGACATCGTCATCTGCCGCCAGCTTCTGTGCCGTAGCCCAGACGCGGCGAAAATGACACACGTCATGGGCCGCGTCCTGGTGCTGATGATGGTTTTTCAACCAGTTTTCGAACTGTGCCTGCCAGTGTTGTAAGTCCATACGCGCTCCGTTGCCAAAGAGTCGCCACCTTAGCAGT  >  NC_000913/2032829‑2033116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A