Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,386,502	T→C	S215P (TCC→CCC)	yfbL →	putative M28A family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,386,502	0	T	C	100.0%	124.3 / NA	35	S215P (TCC→CCC)	yfbL	putative M28A family peptidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/25); total (10/25)

AACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGT  >  NC_000913/2386356‑2386648

aaCCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTg                                                                                                                                                >  2:431844/1‑151 (MQ=255)

     cccTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCttt                                                                                                                                           >  2:606541/1‑151 (MQ=255)

      ccTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTa                                                                                                                                          <  2:450180/151‑1 (MQ=255)

         tcttTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATcc                                                                                                                                       >  1:150442/1‑151 (MQ=255)

              cGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGAt                                                                                                                                   <  1:588196/150‑1 (MQ=255)

               gTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCg                                                                                                                                 <  2:704982/151‑1 (MQ=255)

                   cGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCgggg                                                                                                                              >  1:725390/1‑150 (MQ=255)

                    gATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGgag                                                                                                                            <  1:34472/151‑1 (MQ=255)

                    gATGAAATGGGGAGCGCGGAGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGCGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCgggg                                                                                                                              >  1:280322/1‑149 (MQ=255)

                        aaaTGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACtt                                                                                                                        >  1:535012/1‑151 (MQ=255)

                         aaTGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACttt                                                                                                                       <  1:487732/151‑1 (MQ=255)

                              ggAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGc                                                                                                                  >  1:679890/1‑151 (MQ=255)

                                          atgcaGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAg                                                                                                      <  2:288286/151‑1 (MQ=255)

                                                     cTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGgag                                                                                                                            >  1:22902/1‑118 (MQ=255)

                                                     cTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGgag                                                                                                                            <  2:22902/118‑1 (MQ=255)

                                                        gAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATc                                                                                         <  1:92374/150‑1 (MQ=255)

                                                              ccAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCg                                                                                  <  2:269926/151‑1 (MQ=255)

                                                                            gATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTaaa                                                                     <  2:75365/150‑1 (MQ=255)

                                                                                    tGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCAt                                                             >  2:681970/1‑150 (MQ=255)

                                                                                     ggAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCAtt                                                            <  2:685414/150‑1 (MQ=255)

                                                                                      gagaTGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCAttgt                                                          <  1:306602/151‑1 (MQ=255)

                                                                                              tGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCAtc                                                   <  1:632769/150‑1 (MQ=255)

                                                                                                gCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCa                                                <  2:444344/151‑1 (MQ=255)

                                                                                                   aTTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAg                                               <  1:171486/149‑1 (MQ=255)

                                                                                                      aCGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGAtt                                           <  1:376153/150‑1 (MQ=255)

                                                                                                           tcagcgcCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGAATTTATTGTCGTGGTCGGAAGAATACAGGATATCAACGCCGTACGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGACTTa                                         <  2:743953/144‑1 (MQ=255)

                                                                                                               cgcCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTa                                 <  2:761936/151‑1 (MQ=255)

                                                                                                                gcCTGGAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTAt                                <  1:646901/151‑1 (MQ=255)

                                                                                                                    ggAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTa                            <  2:8091/151‑1 (MQ=255)

                                                                                                                    ggAAGCCAGAATTACCCTTACCCGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTa                            <  1:50365/151‑1 (MQ=255)

                                                                                                                                tacccttacccGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCa                 <  2:679849/150‑1 (MQ=255)

                                                                                                                                 acccttacccGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAg                <  1:290068/150‑1 (MQ=255)

                                                                                                                                   ccttacccGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGt             <  1:102501/151‑1 (MQ=255)

                                                                                                                                    cttacccGGCAATGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGtt            >  2:578642/1‑151 (MQ=255)

                                                                                                                                              aaTGCCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGt  <  2:739675/151‑1 (MQ=255)

AACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGT  >  NC_000913/2386356‑2386648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A