Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	590,771	C→T	D62N (GAC→AAC)	nfrA ←	bacteriophage N4 receptor, outer membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	590,771	0	C	T	100.0%	166.9 / NA	43	D62N (GAC→AAC)	nfrA	bacteriophage N4 receptor, outer membrane subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/21); total (22/21)

GACGTTTCAGTTGATCCTCAAGCAACAGCCGCGCCCGGTCATCATGACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACC  >  NC_000913/590624‑590915

gACGTTTCAGTTGATCCTCAAGCAACAGCCGCGCCCGGTCATCATGACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCgttgtt                                                                                                                                               >  1:158045/1‑151 (MQ=255)

 aCGTTTCAGTTGATCCTCAAGCAACAGCCGCGCCCGGTCATCATGACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCgttgtt                                                                                                                                               >  1:623430/1‑150 (MQ=255)

                  cAAGCAACAGCCGCGCCCGGTCATCATGACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTc                                                                                                                               <  2:183678/149‑1 (MQ=255)

                       aaCAGCCGCGCCCGGTCATCATGACCAAAATGGCGATAGGCTTCCGCAAGGCATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCtt                                                                                                                        >  2:679676/1‑151 (MQ=255)

                        aCAGCCGCGCCCGGTCATCATGACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCttt                                                                                                                       <  2:211773/151‑1 (MQ=255)

                           gCCGCGCCCGGTCATCATGACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATc                                                                                                                    >  2:448296/1‑151 (MQ=255)

                              gcgcCCGGTCATCATGACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCg                                                                                                                   >  2:158605/1‑149 (MQ=255)

                                gcCCGGTCATCATGACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGGGCTTTATCGAGAc                                                                                                               >  2:31868/1‑151 (MQ=255)

                                   cGGTCATCATGACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACggg                                                                                                            >  2:574788/1‑151 (MQ=255)

                                    ggTCATCATGACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACgggg                                                                                                           >  1:27771/1‑151 (MQ=255)

                                           atGACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGataaata                                                                                                    <  2:333596/151‑1 (MQ=255)

                                           atGACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGataaat                                                                                                     <  2:213643/150‑1 (MQ=255)

                                             gACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAAc                                                                                                  >  1:323924/1‑151 (MQ=255)

                                               ccAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACaa                                                                                                <  2:462140/151‑1 (MQ=255)

                                                 aaaaTGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACaaaa                                                                                              <  1:674906/151‑1 (MQ=255)

                                                      ggCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGc                                                                                          <  1:509472/150‑1 (MQ=255)

                                                             ggCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTTCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAAt                                                                                    >  2:339294/1‑149 (MQ=255)

                                                              gCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCTGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGc                                                                                 <  2:281363/151‑1 (MQ=255)

                                                              gCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGc                                                                                 <  2:356373/151‑1 (MQ=255)

                                                                    gCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGcc                                                                           <  1:53729/151‑1 (MQ=255)

                                                                       aGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGcccc                                                                         >  1:29024/1‑150 (MQ=255)

                                                                             aaaGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGctct                                                                   <  1:205138/150‑1 (MQ=255)

                                                                             aaaGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGctct                                                                   <  1:115001/150‑1 (MQ=255)

                                                                                     cGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTg                                                          >  1:556969/1‑151 (MQ=255)

                                                                                      ggAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTgg                                                         <  2:438119/151‑1 (MQ=255)

                                                                                       gAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGt                                                        <  2:655569/151‑1 (MQ=255)

                                                                                        aaTATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTg                                                       <  2:283544/151‑1 (MQ=255)

                                                                                         atatTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGc                                                      >  1:14157/1‑151 (MQ=255)

                                                                                           attatCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCg                                                    <  2:170537/151‑1 (MQ=255)

                                                                                            ttatCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTg                                                       >  2:43519/1‑147 (MQ=255)

                                                                                            ttatCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCg                                                    >  1:656530/1‑150 (MQ=255)

                                                                                                  ggCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGt                                             <  2:147157/151‑1 (MQ=255)

                                                                                                  ggCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGt                                             <  1:156041/151‑1 (MQ=255)

                                                                                                      ccTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGc                                         <  2:519484/151‑1 (MQ=255)

                                                                                                           tgGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGCGAGTg                                    >  1:100234/1‑151 (MQ=255)

                                                                                                            ggTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGCGAGTgc                                   >  1:504013/1‑151 (MQ=255)

                                                                                                             gtgtATATATTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGCGAGTgcg                                  >  1:516558/1‑151 (MQ=255)

                                                                                                                 atatatTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGCGAGTGCGCTg                               <  1:354692/150‑1 (MQ=255)

                                                                                                                   atatTCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGCGAGTGCGCTGGt                             >  2:486190/1‑150 (MQ=255)

                                                                                                                       tCAAATTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGCGAGTGCGCTGGTACTc                         >  2:244883/1‑150 (MQ=255)

                                                                                                                          aaTTCGCGGATGGCGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGCGAGTGCGCTGGTACTCAAt                      >  1:449129/1‑150 (MQ=255)

                                                                                                                                       cGGTTGCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGCGAGTGCGCTGGTACTCAATAAAGACGTcagcag        <  2:529144/151‑1 (MQ=255)

                                                                                                                                            gCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAAc   >  1:500684/1‑151 (MQ=255)

                                                                                                                                            gCTTCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAAc   >  2:93209/1‑151 (MQ=255)

                                                                                                                                              ttCGTTGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAAcc  >  2:665240/1‑150 (MQ=255)

GACGTTTCAGTTGATCCTCAAGCAACAGCCGCGCCCGGTCATCATGACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTGCGCTGGTGCCGATATTGTCTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACC  >  NC_000913/590624‑590915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A