breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 14,991 C→T A275V (GCG→GTG)  dnaJ → chaperone Hsp40, DnaK co‑chaperone
RA 39,967 A→G Y140Y (TAT→TAC caiA ← crotonobetaine reductase subunit II, FAD‑binding
RA 44,792 A→G N205D (AAT→GAT)  fixC → putative oxidoreductase
RA 134,458 T→C V24A (GTC→GCC)  yacL → UPF0231 family protein
RA 143,271 G→A A165T (GCA→ACA)  yadG → putative ABC transporter ATPase
RA 232,838 C→T G373E (GGA→GAA)  mltD ← putative membrane‑bound lytic murein transglycosylase D
RA 249,034 G→A pseudogene (1037/1713 nt) lfhA ← pseudogene, flagellar system protein, promoterless fragment; flagellar biosynthesis
MC JC 257,908 Δ776 bp [crl] [crl]
RA 283,790 A→G D197G (GAC→GGC)  yagF → CP4‑6 prophage; dehydratase family protein
RA 284,450 A→G E417G (GAG→GGG)  yagF → CP4‑6 prophage; dehydratase family protein
RA 285,648 C→G A85G (GCC→GGC)  yagG → CP4‑6 prophage; putative sugar transporter
JC 308,054 (C)7→10 coding (1279/2526 nt) ecpC ← ECP production outer membrane protein
MC JC 381,211 Δ1,383 bp [yaiX][yaiX] [yaiX], insC1, insD1, [yaiX]
RA 474,975 A→G Y63H (TAC→CAC)  tesB ← acyl‑CoA thioesterase 2
RA 483,535 C→T G290E (GGG→GAG)  acrB ← multidrug efflux system protein
RA 484,337 (C)6→7 coding (67/3150 nt) acrB ← multidrug efflux system protein
RA 545,039 A→G V85A (GTG→GCG)  allC ← allantoate amidohydrolase
JC JC 572,172 IS2 (–) +5 bp coding (719‑723/798 nt) ybcM → DLP12 prophage; putative DNA‑binding transcriptional regulator
RA 581,913 (A)6→7 pseudogene (254/438 nt) tfaD → pseudogene, DLP12 prophage; tail fiber assembly protein family;Phage or Prophage Related
RA 590,771 C→T D62N (GAC→AAC)  nfrA ← bacteriophage N4 receptor, outer membrane subunit
RA 657,377 T→C V29A (GTG→GCG)  cspE → constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
RA 667,534 T→C E228E (GAA→GAG mrdA ← penicillin‑binding protein 2, transpeptidase involved in peptidoglycan synthesis
RA 699,916 A→G G137G (GGT→GGC umpH ← UMP phosphatase
RA 702,597 (G)5→6 coding (155/1149 nt) nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 736,382 T→C intergenic (+163/‑63) ybfC → / → ybfQ putative secreted protein/pseudogene, H repeat‑associated protein
RA 738,795 C→T T235I (ACC→ATC)  ybfD → H repeat‑associated putative transposase
RA 738,829 G→A E246E (GAG→GAA ybfD → H repeat‑associated putative transposase
RA 758,260 A→G D191G (GAC→GGC)  sdhB → succinate dehydrogenase, FeS subunit
RA 773,267 C→T P76S (CCG→TCG)  cydB → cytochrome d terminal oxidase, subunit II
RA 798,120 A→G G104G (GGT→GGC ybhA ← pyridoxal phosphate (PLP) phosphatase
RA 817,261 A→G E73G (GAG→GGG)  moaA → molybdopterin biosynthesis protein A
RA 838,311 +G coding (146/267 nt) ybiI ← DksA‑type zinc finger protein
RA 841,490 C→T S14S (TCG→TCA fiu ← catecholate siderophore receptor
RA 845,744 A→G S240S (TCT→TCC glnQ ← glutamine transporter subunit
RA 858,789 T→C K91K (AAA→AAG ybiU ← DUF1479 family protein
RA 867,112 C→G A182G (GCC→GGC)  iaaA → Isoaspartyl peptidase
RA 892,409 +G coding (443/903 nt) rimK → ribosomal protein S6 modification protein
RA 932,884 T→C V97A (GTA→GCA)  lrp → leucine‑responsive global transcriptional regulator
RA 942,738 C→T R594W (CGG→TGG)  dmsA → dimethyl sulfoxide reductase, anaerobic, subunit A
RA 958,916 A→G A35A (GCA→GCG aroA → 5‑enolpyruvylshikimate‑3‑phosphate synthetase
RA 997,179 C→T A112T (GCC→ACC)  ssuE ← NAD(P)H‑dependent FMN reductase
RA 1,013,706 T→C L150L (TTG→CTG)  pqiB → paraquat‑inducible, SoxRS‑regulated MCE domain protein
RA 1,013,807 A→G V183V (GTA→GTG pqiB → paraquat‑inducible, SoxRS‑regulated MCE domain protein
RA 1,022,584 T→C T434A (ACG→GCG)  yccS ← putative transporter, FUSC superfamily inner membrane protein
RA 1,053,037 A→G V109A (GTC→GCC)  yccM ← putative 4Fe‑4S membrane protein
RA 1,088,769 C→T G363G (GGG→GGA pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
RA 1,124,108 (G)6→7 intergenic (‑54/+10) grxB ← / ← mdtH glutaredoxin 2 (Grx2)/multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
RA 1,132,321 C→T T156T (ACC→ACT flgD → flagellar hook assembly protein
RA 1,163,578 G→A A107T (GCA→ACA)  thiK → thiamine kinase
RA 1,172,139 A→G I609T (ATT→ACT)  mfd ← transcription‑repair coupling factor
RA 1,203,537 C→T D90D (GAC→GAT ymfL → e14 prophage; putative DNA‑binding transcriptional regulator
RA 1,214,838 T→C E213G (GAA→GGA)  bluF ← anti‑repressor for YcgE, blue light‑responsive; FAD‑binding; inactive c‑di‑GMP phosphodiesterase‑like EAL domain protein
RA 1,225,326 C→T G12G (GGG→GGA minD ← inhibitor of FtsZ ring polymerization; chromosome‑membrane tethering protein; membrane ATPase of the MinCDEE system
RA 1,270,311 T→C H12R (CAC→CGC)  ldrC ← toxic polypeptide, small
RA 1,281,664 C→T R601C (CGT→TGT)  narG → nitrate reductase 1, alpha subunit
RA 1,290,753 G→A C171Y (TGC→TAC)  rssB → PcnB‑degradosome interaction factor; response regulator
RA 1,307,516 A→G V377A (GTC→GCC)  clsA ← cardiolipin synthase 1
RA 1,308,099 C→T G183R (GGG→AGG)  clsA ← cardiolipin synthase 1
RA 1,324,641 A→G T182A (ACA→GCA)  yciO → putative RNA binding protein
RA 1,358,190 A→G V333A (GTG→GCG)  puuP ← putrescine importer
RA 1,380,716 T→A I190N (ATC→AAC)  ycjU → beta‑phosphoglucomutase
RA 1,382,785 A→G Q280R (CAG→CGG)  ompG → outer membrane porin G
RA 1,383,697 C→T A89T (GCC→ACC)  ycjW ← LacI family putative transcriptional repressor
RA 1,392,489 G→A A167T (GCT→ACT)  pgrR → murein peptide degradation regulator
RA 1,393,223 A→G intergenic (+333/‑4) pgrR → / → mppA murein peptide degradation regulator/murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
RA 1,407,641 A→G V52A (GTC→GCC)  ydaM ← diguanylate cyclase, csgD regulator
RA 1,419,721 (G)7→6 intergenic (‑265/+44) ydaG ← / ← racR Rac prophage; uncharacterized protein/Rac prophage; putative DNA‑binding transcriptional regulator
RA 1,420,793 (G)6→7 coding (110/423 nt) ydaT → Rac prophage; uncharacterized protein
RA 1,444,706 C→T G552G (GGC→GGT ydbH → putative membrane‑anchored protein, function unknown
RA 1,448,822 T→C V435A (GTC→GCC)  feaB → phenylacetaldehyde dehydrogenase
RA 1,449,092 A→G L753P (CTG→CCG)  tynA ← tyramine oxidase, copper‑requiring
RA 1,570,650 T→C T466A (ACC→GCC)  gadB ← glutamate decarboxylase B, PLP‑dependent
RA 1,580,435 C→G G119A (GGC→GCC)  ydeM ← putative YdeN‑specific sulfatase‑maturating enzyme
RA 1,595,660 A→G pseudogene (2328/3861 nt) yneO ← pseudogene, AidA homolog
RA 1,603,562 T→C F182L (TTT→CTT)  lsrC → autoinducer 2 import system permease protein
RA 1,646,854 C→T intergenic (+117/+320) flxA → / ← intK Qin prophage; uncharacterized protein/pseudogene, integrase fragment, Qin prophage;Phage or Prophage Related
RA 1,691,784 A→G S356S (AGT→AGC uidC ← putative outer membrane porin for beta‑glucuronides porin protein
RA 1,717,165 T→C T20A (ACG→GCG)  tyrS ← tyrosyl‑tRNA synthetase
MC JC 1,755,661 Δ2,703 bp pykF[ldtE] pykF, lpp, [ldtE]
RA 1,769,222 C→T P50L (CCG→CTG)  ydiK → UPF0118 family inner membrane protein
RA 1,803,248 T→C pseudogene (155/426 nt) arpB → pseudogene, ankyrin repeats
RA 1,844,948 T→A intergenic (‑77/+51) ynjI ← / ← topB inner membrane protein/DNA topoisomerase III
RA 1,877,132 A→G H75R (CAC→CGC)  yeaO → DUF488 family protein
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 family protein, nitrate‑inducible
RA 1,915,005 T→C H177R (CAC→CGC)  proQ ← RNA chaperone, putative ProP translation regulator
RA 1,916,069 T→C G20G (GGA→GGG msrC ← free methionine‑(R)‑sulfoxide reductase
RA 1,932,865 T→C G580G (GGA→GGG edd ← 6‑phosphogluconate dehydratase
RA 1,946,069 T→C intergenic (‑93/+46) ruvA ← / ← yobI component of RuvABC resolvasome, regulatory subunit/uncharacterized protein
RA 1,955,053 T→C H652R (CAC→CGC)  torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 1,982,132 G→A R86C (CGC→TGC)  otsB ← trehalose‑6‑phosphate phosphatase, biosynthetic
RA 1,994,904 T→C E152E (GAA→GAG uvrY ← response regulator in two‑component regulatory system with BarA
RA 2,017,429 (C)6→7 coding (876/1374 nt) fliI → flagellum‑specific ATP synthase
RA 2,063,976 T→C A164A (GCA→GCG cobT ← nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
RA 2,088,373 G→A G252G (GGC→GGT yeeZ ← putative epimerase
RA 2,129,612 G→A L13L (CTC→CTT wcaE ← putative glycosyl transferase
RA 2,151,290 T→C S36P (TCT→CCT)  yegJ → uncharacterized protein
RA 2,161,380 T→C Y999H (TAT→CAT)  mdtC → multidrug efflux system, subunit C
RA 2,168,009 T→C T100A (ACA→GCA)  yegR ← uncharacterized protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,173,933 T→C pseudogene (346/914 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,175,182 A→G S380S (AGT→AGC gatZ ← D‑tagatose 1,6‑bisphosphate aldolase 2, subunit
RA 2,223,301 T→C F373L (TTC→CTC)  dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent
RA 2,258,212 T→C E52E (GAA→GAG psuG ← pseudouridine 5'‑phosphate glycosidase
RA 2,261,739 T→C A209A (GCA→GCG fruK ← fructose‑1‑phosphate kinase
RA 2,269,424 T→C W191R (TGG→CGG)  lpxT → lipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase
RA 2,325,637 T→C R294R (CGT→CGC atoE → short chain fatty acid transporter
RA 2,339,460 C→T intergenic (‑40/‑107) gyrA ← / → ubiG DNA gyrase (type II topoisomerase), subunit A/bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase/ 2‑octaprenyl‑6‑hydroxy phenol methylase
RA 2,367,405 G→A R112H (CGC→CAC)  arnC → undecaprenyl phosphate‑L‑Ara4FN transferase
RA 2,379,241 T→A N7Y (AAC→TAC)  menD ← 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase
RA 2,435,409 C→T A76A (GCG→GCA truA ← tRNA pseudouridine(38‑40) synthase
RA 2,441,945 T→C V61A (GTG→GCG)  mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
RA 2,454,272 A→G pseudogene (730/2646 nt) yfcU ← pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein
RA 2,471,762 G→C pseudogene (361/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,476,116 A→T I82F (ATC→TTC)  yfdT → CPS‑53 (KpLE1) prophage; uncharacterized protein
RA 2,477,942 G→A A32A (GCG→GCA dsdX → D‑serine transporter
RA 2,536,495 G→A A248V (GCG→GTG)  pdxK ← pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase
RA 2,595,335 T→C A175A (GCA→GCG tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase
RA 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4, membrane subunit
RA 2,616,013 T→C intergenic (‑132/‑81) yfgO ← / → bepA putative UPF0118 family inner membrane permease/OM protein maintenance and assembly metalloprotease and chaperone, periplasmic
RA 2,682,157 (C)7→8 coding (589/3282 nt) yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,687,347 G→A A21V (GCC→GTC)  glnB ← regulatory protein P‑II for glutamine synthetase
RA 2,688,468 C→T A112A (GCG→GCA glrR ← response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK
RA 2,747,020 G→A A259V (GCG→GTG)  ffh ← Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)
RA 2,764,549 T→C D202G (GAC→GGC)  yfjL ← CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein
RA 2,765,124 G→A A10A (GCC→GCT yfjL ← CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein
RA 2,801,859 A→G Q171R (CAG→CGG)  nrdE → ribonucleoside‑diphosphate reductase 2, alpha subunit
RA 2,805,310 T→C S166P (TCT→CCT)  proV → glycine betaine/proline ABC transporter periplasmic binding protein
RA 2,809,534 T→C intergenic (+41/‑83) ygaY → / → ygaZ pseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein/putative L‑valine exporter, norvaline resistance protein
RA 2,812,356 T→C G310G (GGT→GGC emrA → multidrug efflux system
RA 2,858,929 A→C T613P (ACC→CCC)  mutS → methyl‑directed mismatch repair protein
RA 2,913,035 A→G L206P (CTC→CCC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,913,196 T→C K152K (AAA→AAG relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,968,723 A→G R85R (CGT→CGC rppH ← RNA pyrophosphohydrolase
JC 3,018,356 (GTC)4→3 coding (2297‑2299/3099 nt) ygfK → putative Fe‑S subunit oxidoreductase subunit
RA 3,036,196 G→A A4V (GCC→GTC)  prfB ← peptide chain release factor RF‑2
RA 3,037,738 A→T D123E (GAT→GAA recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific
RA 3,068,614 C→T S65S (TCG→TCA argO ← arginine transporter
RA 3,088,189 T→C intergenic (+329/‑95) metK → / → galP S‑adenosylmethionine synthetase/D‑galactose transporter
RA 3,139,485 A→G K253K (AAA→AAG yghU → putative S‑transferase
RA 3,158,129 C→T A160A (GCC→GCT yqhG → DUF3828 family putative periplasmic protein
RA 3,162,669 T→C M1M (ATG→GTG) † ftsP ← septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)
RA 3,163,010 G→A P158S (CCG→TCG)  plsC ← 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase
RA 3,175,651 G→A S119L (TCA→TTA)  yqiA ← acyl CoA esterase
RA 3,211,471 G→A G122D (GGT→GAT)  dnaG → DNA primase
RA 3,235,399 G→A L159L (CTC→CTT rlmG ← 23S rRNA m(2)G1835 methyltransferase, SAM‑dependent
RA 3,252,977 T→C Y23H (TAC→CAC)  yhaI → DUF805 family inner membrane protein
RA 3,362,012 A→G intergenic (+72/‑100) gltF → / → yhcA periplasmic protein/putative periplasmic chaperone protein
RA 3,368,566 G→A G247E (GGG→GAG)  yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
RA 3,391,786 A→G A81A (GCT→GCC tldD ← putative peptidase
RA 3,439,982 A→G S6S (AGT→AGC rplQ ← 50S ribosomal subunit protein L17
RA 3,457,324 T→C D316D (GAT→GAC gspD → general secretory pathway component, cryptic
RA 3,462,301 A→G T110A (ACG→GCG)  gspI → general secretory pathway component, cryptic
RA 3,466,888 A→G V35A (GTC→GCC)  bfd ← bacterioferritin‑associated ferredoxin
RA 3,497,615 T→C F205L (TTC→CTC)  nirC → nitrite transporter
RA 3,505,884 A→G L46P (CTG→CCG)  yhfS ← FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,532,256 +T coding (184/1725 nt) yhgE ← DUF4153 family putative inner membrane protein
RA 3,546,644 T→C V29A (GTC→GCC)  gntT → gluconate transporter, high‑affinity GNT I system
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,563,193 A→G D394G (GAC→GGC)  glpD → sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
RA 3,572,038 T→C T489A (ACC→GCC)  glgB ← 1,4‑alpha‑glucan branching enzyme
RA 3,572,259 T→C Y415C (TAC→TGC)  glgB ← 1,4‑alpha‑glucan branching enzyme
RA 3,575,146 T→C S26P (TCC→CCC)  yhgN → UPF0056 family inner membrane protein
RA 3,578,677 A→G V17A (GTG→GCG)  gntR ← d‑gluconate inducible gluconate regulon transcriptional repressor
RA 3,629,003 T→C D179G (GAC→GGC)  rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
RA 3,648,144 (T)7→8 intergenic (‑356/‑384) dinQ ← / → arsR UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/arsenical resistance operon transcriptional repressor; autorepressor
RA 3,689,711 G→A P184L (CCG→CTG)  bcsZ ← endo‑1,4‑D‑glucanase
RA 3,696,621 A→G H55R (CAC→CGC)  bcsE → cellulose production protein
RA 3,700,370 A→G intergenic (‑283/‑193) ldrD ← / → yhjV toxic polypeptide, small/putative transporter
RA 3,715,195 T→C E400E (GAA→GAG bisC ← biotin sulfoxide reductase
RA 3,779,043 A→G M549V (ATG→GTG)  lldP → L‑lactate permease
RA 3,815,521 A→G V83A (GTG→GCG)  pyrE ← orotate phosphoribosyltransferase
RA 3,815,808 (A)8→7 intergenic (‑40/+26) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,843,430 C→T W120* (TGG→TGA adeQ ← adenine permease, high affinity; adenine:H+ symporter
RA 3,844,146 T→C G61G (GGT→GGC adeD → cryptic adenine deaminase
RA 3,859,124 A→G G331G (GGT→GGC yidK ← putative transporter
RA 3,878,843 T→C D426G (GAC→GGC)  gyrB ← DNA gyrase, subunit B
RA 3,889,885 T→C Y386H (TAT→CAT)  tnaA → tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
RA 3,895,826 A→G L262L (TTG→CTG)  adeP ← adenine permease, high affinity; adenine:H+ symporter
RA 3,928,167 T→C R479R (AGA→AGG viaA ← stimulator of RavA ATPase activity; von Willebrand factor domain protein
RA 3,947,067 (T)8→7 intergenic (+35/+61) trpT → / ← hdfR tRNA‑Trp/flhDC operon transcriptional repressor
RA 3,967,132 A→G H239R (CAC→CGC)  rho → transcription termination factor
RA 3,979,674 T→C G358G (GGT→GGC wzyE → putative ECA polysaccharide chain elongation protein
RA 4,032,787 T→C V99A (GTA→GCA)  yigZ → UPF0029 family protein
RA 4,114,622 A→G S320P (TCC→CCC)  glpX ← fructose 1,6‑bisphosphatase II
RA 4,128,247 C→T R50H (CGT→CAT)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding
RA 4,134,394 A→G D187G (GAC→GGC)  katG → catalase‑peroxidase HPI, heme b‑containing
RA 4,139,721 Δ1 bp coding (2501/2502 nt) frwA ← putative PTS enzyme: Hpr, enzyme I and II components
RA 4,157,132 A→G K95E (AAA→GAA)  argH → argininosuccinate lyase
RA 4,173,601 C→T P174S (CCT→TCT)  birA → bifunctional biotin‑[acetylCoA carboxylase] holoenzyme synthetase/ DNA‑binding transcriptional repressor, bio‑5'‑AMP‑binding
RA 4,237,614 A→G T240A (ACG→GCG)  yjbG → extracellular polysaccharide export OMA protein
RA 4,281,575 (A)5→4 coding (1596/1650 nt) yjcE → putative cation/proton antiporter
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,320,065 T→C D200G (GAC→GGC)  phnI ← ribophosphonate triphosphate synthase complex putative catalytic subunit
RA 4,334,049 T→C Q514Q (CAA→CAG eptA ← lipid A phosphoethanolamine transferase
RA 4,339,313 A→G R403R (CGT→CGC adiA ← arginine decarboxylase
RA 4,341,617 A→T D4E (GAT→GAA melR ← melibiose operon transcriptional regulator; autoregulator
RA 4,359,666 A→G G122G (GGT→GGC cadB ← putative lysine/cadaverine transporter
RA 4,381,504 T→C E272G (GAA→GGA)  frdA ← anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit
RA 4,391,867 A→G A88A (GCA→GCG orn → oligoribonuclease
RA 4,407,982 T→C R443R (CGT→CGC rnr → exoribonuclease R, RNase R
RA 4,415,887 (G)6→7 coding (1613/1626 nt) aidB → DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
RA 4,485,819 C→T A45T (GCC→ACC)  pepA ← multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
RA 4,491,790 A→G L246L (TTA→CTA)  idnT ← L‑idonate and D‑gluconate transporter
RA 4,494,883 (A)7→8 coding (261/564 nt) idnK → D‑gluconate kinase, thermosensitive
RA 4,583,861 A→G R967R (CGT→CGC hsdR ← endonuclease R Type I restriction enzyme
RA 4,606,331 A→G noncoding (71/87 nt) leuQ ← tRNA‑Leu
RA 4,620,670 T→C G356G (GGT→GGC deoB → phosphopentomutase
RA 4,641,366 T→C L142P (CTC→CCC)  yjtD → putative methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 226131–226601 228595–226988 388–2465 18 [16] [17] 18 rrlH 23S ribosomal RNA of rrnH operon
* * ÷ NC_000913 738053 738766 714 18 [16] [17] 18 [ybfD] [ybfD]
* * ÷ NC_000913 3423727–3424232 3424553–3424238 7–827 18 [17] [17] 18 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =3 (0.070)36 (0.930) 30/266 0.5 89.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 6 (0.160)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078053 (0.070)26 (0.670) 26/266 0.8 85.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 6 (0.160)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)25 (0.590) 24/290 1.3 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 46062078 (0.200)28 (0.660)
+GGG
26/276 0.9 77.6% noncoding (80/87 nt) leuP tRNA‑Leu
?NC_000913 4606208 = 8 (0.180)noncoding (79/87 nt) leuP tRNA‑Leu