Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 1,755,661 Δ2,703 bp pykF[ldtE] pykF, lpp, [ldtE]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1755661 1758363 2703 49 [0] [1] 50 pykF–[ldtE] pykF, lpp, [ldtE]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 17556600 (0.000)49 (1.140) 45/296 0.1 99.0% intergenic (‑519/‑38) ydhZ/pykF uncharacterized protein/pyruvate kinase I
?NC_000913 1758364 = 1 (0.020)coding (362/1005 nt) ldtE murein L,D‑transpeptidase

GACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1755515‑1755660
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCG  >  NC_000913/1758364‑1758510
                                                                                                                                                                                                                                                                                                     
GACTCAAAAACAGGAACTCACGTTGGGCTGAGACACAAGCAAAAATTCGTATGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGAGCACAGAAAGCAAGTGGAT                                                                                                                                                 >  1:422850/1‑150
 ACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATA                                                                                                                                                >  2:50395/1‑150
        AACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCA                                                                                                                                        <  1:19812/151‑1
                CTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTT                                                                                                                                >  2:379606/1‑151
                  CACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTg                                                                                                                                <  2:421424/149‑2
                  CACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTC                                                                                                                              <  2:624600/151‑1
                  CACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTT                                                                                                                               <  2:195941/150‑1
                     GTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCC                                                                                                                           <  1:346056/151‑1
                       cGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCGGATAAACCTGCACAATATTTTCTCCCG                                                                                                                         >  2:76296/2‑151
                        GGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCG                                                                                                                         <  1:511426/150‑1
                          GCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCG                                                                                                                      >  1:166377/1‑151
                            TGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGG                                                                                                                     >  2:192444/1‑150
                             GAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGA                                                                                                                    <  2:170615/150‑1
                             GAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGG                                                                                                                     <  1:231340/149‑1
                                     AGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAA                                                                                                           <  1:36034/151‑1
                                      GCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAA                                                                                                          <  2:452798/151‑1
                                       CACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCGCAATATTTTCTCCCGGCGGATAATAATAAAG                                                                                                         <  1:538595/151‑1
                                       CACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAG                                                                                                         <  2:601592/151‑1
                                         CACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGC                                                                                                       <  1:611173/151‑1
                                           CATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGC                                                                                                     <  1:138886/151‑1
                                            ATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCA                                                                                                    <  2:340008/151‑1
                                             TTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGC                                                                                                     >  1:543304/1‑149
                                              TCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAG                                                                                                   <  2:355349/150‑1
                                                       CGCTTTTTCGATGTCACCTATCCTTAGAGCTAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTCTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGG                                                                                         <  2:334627/151‑1
                                                           TTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTA                                                                                      >  1:6901/1‑150
                                                              TCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACG                                                                                   >  2:343211/1‑150
                                                                  TGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAAT                                                                              >  2:390209/1‑151
                                                                   GTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAAT                                                                              <  1:577118/150‑1
                                                                     CACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCC                                                                           >  2:606451/1‑151
                                                                      ACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCC                                                                          >  2:587704/1‑151
                                                                       CCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCT                                                                         <  2:190526/151‑1
                                                                             CTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCG                                                                   >  1:424115/1‑151
                                                                             CTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCG                                                                   >  2:276765/1‑151
                                                                               TAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCG                                                                   <  1:226833/149‑1
                                                                                AGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTG                                                                <  1:272630/151‑1
                                                                                AGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGT                                                                 <  1:305845/150‑1
                                                                                   GCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCAT                                                             <  1:390220/151‑1
                                                                                            CACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAAT                                                     >  1:447334/1‑150
                                                                                                ACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAG                                                <  1:394240/151‑1
                                                                                                     CGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTG                                           >  2:120237/1‑151
                                                                                                     CGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTG                                           <  2:402944/151‑1
                                                                                                         ATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGG                                       <  2:92858/151‑1
                                                                                                                ATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTT                                <  1:665113/151‑1
                                                                                                                            GCAGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACC                     >  2:579062/1‑150
                                                                                                                              AGTGCGCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGG                  >  1:649396/1‑151
                                                                                                                                   GCCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTG             >  2:334038/1‑151
                                                                                                                                    CCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGG            >  1:96054/1‑151
                                                                                                                                    CCCAGAAAGCAAGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGG            <  1:513633/151‑1
                                                                                                                                               AGTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCG  >  2:280927/1‑150
                                                                                                                                                                                                                                                                                                     
GACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1755515‑1755660
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCG  >  NC_000913/1758364‑1758510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.