Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,755,661	Δ2,703 bp		pykF–[ldtE]	pykF, lpp, [ldtE]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1755661	1758363	2703	49 [0]	[1] 50	pykF–[ldtE]	pykF, lpp, [ldtE]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1755660	0 (0.000)	49 (1.140)	45/296	0.1	99.0%	intergenic (‑519/‑38)	ydhZ/pykF	uncharacterized protein/pyruvate kinase I
	?	NC_000913	1758364 =	1 (0.020)					coding (362/1005 nt)	ldtE	murein L,D‑transpeptidase

GACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCA  >  NC_000913/1755515‑1755807                                                                                                                                                  |                                                                                                                                                    gACTCAAAAACAGGAACTCACGTTGGGCTGAGACACAAGCAAAAATTCGTATGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGAGCACAGAAAGCAAGTggat                                                                                                                                                 >  1:422850‑M1/1‑146 (MQ=255)  aCTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggata                                                                                                                                                >  2:50395‑M1/1‑145 (MQ=255)         aaCAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgca                                                                                                                                        <  1:19812‑M1/151‑14 (MQ=255)                 cTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattt                                                                                                                                >  2:379606‑M1/1‑130 (MQ=255)                   cACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttc                                                                                                                              <  2:624600‑M1/151‑24 (MQ=255)                   cACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatatttt                                                                                                                               <  2:195941‑M1/150‑23 (MQ=255)                   cACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattg                                                                                                                                <  2:421424‑M1/149‑22 (MQ=255)                      gTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcc                                                                                                                           <  1:346056‑M1/151‑27 (MQ=255)                        cgggcTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCggataaacctgcacaatattttctcccg                                                                                                                         >  2:76296‑M1/2‑123 (MQ=255)                         gggCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccg                                                                                                                         <  1:511426‑M1/150‑29 (MQ=255)                           gCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcg                                                                                                                      >  1:166377‑M1/1‑120 (MQ=255)                             tGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcgg                                                                                                                     >  2:192444‑M1/1‑118 (MQ=255)                              gagaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcgga                                                                                                                    <  2:170615‑M1/150‑34 (MQ=255)                              gagaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcgg                                                                                                                     <  1:231340‑M1/149‑33 (MQ=255)                                      aGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataa                                                                                                           <  1:36034‑M1/151‑43 (MQ=255)                                       gCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaa                                                                                                          <  2:452798‑M1/151‑44 (MQ=255)                                        cacacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcgcaatattttctcccggcggataataataaag                                                                                                         <  1:538595‑M1/151‑45 (MQ=255)                                        cacacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaag                                                                                                         <  2:601592‑M1/151‑45 (MQ=255)                                          cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggc                                                                                                       <  1:611173‑M1/151‑47 (MQ=255)                                            caTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgc                                                                                                     <  1:138886‑M1/151‑49 (MQ=255)                                             aTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgca                                                                                                    <  2:340008‑M1/151‑50 (MQ=255)                                              ttCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgc                                                                                                     >  1:543304‑M1/1‑101 (MQ=255)                                               tCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcag                                                                                                   <  2:355349‑M1/150‑51 (MQ=255)                                                        cGCTTTTTCGATGTCACCTATCCTTAGAGCTAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattctctcccggcggataataataaaggcgcagctctgcaagg                                                                                         <  2:334627‑M1/151‑61 (MQ=255)                                                            ttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggtta                                                                                      >  1:6901‑M1/1‑87 (MQ=255)                                                               tCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacg                                                                                   >  2:343211‑M1/1‑84 (MQ=255)                                                                   tGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataat                                                                              >  2:390209‑M1/1‑80 (MQ=255)                                                                    gTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataat                                                                              <  1:577118‑M1/150‑72 (MQ=255)                                                                      cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatccc                                                                           >  2:606451‑M1/1‑77 (MQ=255)                                                                       aCCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccc                                                                          >  2:587704‑M1/1‑76 (MQ=255)                                                                        ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccct                                                                         <  2:190526‑M1/151‑77 (MQ=255)                                                                              cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcg                                                                   >  2:276765‑M1/1‑69 (MQ=255)                                                                              cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcg                                                                   >  1:424115‑M1/1‑69 (MQ=255)                                                                                tAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcg                                                                   <  1:226833‑M1/149‑83 (MQ=255)                                                                                 agagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtg                                                                <  1:272630‑M1/151‑86 (MQ=255)                                                                                 agagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggt                                                                 <  1:305845‑M1/150‑85 (MQ=255)                                                                                    gCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcat                                                             <  1:390220‑M1/151‑89 (MQ=255)                                                                                             caccacTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaat                                                     >  1:447334‑M1/1‑54 (MQ=255)                                                                                                 acTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacag                                                <  1:394240‑M1/151‑102 (MQ=255)                                                                                                      cGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtg                                           >  2:120237‑M1/1‑45 (MQ=255)                                                                                                      cGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtg                                           <  2:402944‑M1/151‑107 (MQ=255)                                                                                                          aTACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaagg                                       <  2:92858‑M1/151‑111 (MQ=255)                                                                                                                 aTTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagtt                                <  1:665113‑M1/151‑118 (MQ=255)                                                                                                                             gCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagttatcgtcgtacc                     >  2:579062‑M1/1‑22 (MQ=255)                                                                                                                               aGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagttatcgtcgtaccagg                  >  1:649396‑M1/1‑20 (MQ=255)                                                                                                                                    gcCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagttatcgtcgtaccaggttttg             >  2:334038‑M1/1‑15 (MQ=255)                                                                                                                                     cccAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagttatcgtcgtaccaggttttgg            >  1:96054‑M1/1‑14 (MQ=255)                                                                                                                                     cccAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagttatcgtcgtaccaggttttgg            <  1:513633‑M1/151‑138 (MQ=255)                                                                                                                                                aGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagttatcgtcgtaccaggttttggcaccggggcg  >  2:280927‑M1/1‑3 (MQ=255)                                                                                                                                                  |                                                                                                                                                    GACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCA  >  NC_000913/1755515‑1755807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑