| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | NC_000913 | 1,755,661 | Δ2,703 bp | pykF–[ldtE] | pykF, lpp, [ldtE] | |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 1755661 | 1758363 | 2703 | 49 [0] | [1] 50 | pykF–[ldtE] | pykF, lpp, [ldtE] |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 1755660 | 0 (0.000) | 49 (1.140) | 45/296 | 0.1 | 99.0% | intergenic (‑519/‑38) | ydhZ/pykF | uncharacterized protein/pyruvate kinase I |
| ? | NC_000913 | 1758364 = | 1 (0.020) | coding (362/1005 nt) | ldtE | murein L,D‑transpeptidase | |||||
CAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCAACACGCGTTTCCATCACCGGCGTTTCCAGCCCCTGCAATCCAATACCTATTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCG > NC_000913/1758218‑1758510 | gactcaaaaacaggaactcacgttgggctgagacacaagcaaaaattcgtatgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgagcacagaaagcaagtGGAt > 1:422850‑M2/147‑150 (MQ=255) actcaaaaacaggcactcacgttgggctgagacacaagcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATa > 2:50395‑M2/146‑150 (MQ=255) aacaggcactcacgttgggctgagacacaagcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGca < 1:19812‑M2/13‑1 (MQ=255) ctcacgttgggctgagacacaagcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATAttt > 2:379606‑M2/131‑151 (MQ=255) cacgttgggctgagacacaagcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATattg < 2:421424‑M2/21‑2 (MQ=255) cacgttgggctgagacacaagcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATAtttt < 2:195941‑M2/22‑1 (MQ=255) cacgttgggctgagacacaagcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTtc < 2:624600‑M2/23‑1 (MQ=255) gttgggctgagacacaagcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTcc < 1:346056‑M2/26‑1 (MQ=255) cgggctgagacacaagcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagcGGATAAACCTGCACAATATTTTCTCCcg > 2:76296‑M2/124‑151 (MQ=255) gggctgagacacaagcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCcg < 1:511426‑M2/28‑1 (MQ=255) gctgagacacaagcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCcggcg > 1:166377‑M2/121‑151 (MQ=255) tgagacacaagcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCcggcgg > 2:192444‑M2/119‑150 (MQ=255) gagacacaagcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCcggcgg < 1:231340‑M2/32‑1 (MQ=255) gagacacaagcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGa < 2:170615‑M2/33‑1 (MQ=255) agcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGAtaataataa < 1:36034‑M2/42‑1 (MQ=255) gcacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATaaa < 2:452798‑M2/43‑1 (MQ=255) cacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCGCAATATTTTCTCCCGGCGGATAATAATAAAg < 1:538595‑M2/44‑1 (MQ=255) cacacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAg < 2:601592‑M2/44‑1 (MQ=255) acctcTGACGAATGCCTGCCGTATGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCAACACGCGTTTCCATCTCCGGCGTTTCCAGCCCCTGCAACCCAATACCCATTCGATACACCTGCACACTACTTTCTCCCGGCGGatcatattt < 2:313825/144‑4 (MQ=255) cacattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGgc < 1:611173‑M2/46‑1 (MQ=255) cattcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGgcgc < 1:138886‑M2/48‑1 (MQ=255) attcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCa < 2:340008‑M2/49‑1 (MQ=255) ttcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGgcgc > 1:543304‑M2/102‑149 (MQ=255) tcctctgcacgctttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAg < 2:355349‑M2/50‑1 (MQ=255) cgctttttcgatgtcacctatccttagagctaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTCTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAgg < 2:334627‑M2/60‑1 (MQ=255) ttttcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTa > 1:6901‑M2/88‑150 (MQ=255) tcgatgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACg > 2:343211‑M2/85‑150 (MQ=255) tgtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAAt > 2:390209‑M2/81‑151 (MQ=255) gtcacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAAt < 1:577118‑M2/71‑1 (MQ=255) cacctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATccc > 2:606451‑M2/78‑151 (MQ=255) acctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATcccc > 2:587704‑M2/77‑151 (MQ=255) cctatccttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCt < 2:190526‑M2/76‑1 (MQ=255) cttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGAcgcg > 1:424115‑M2/70‑151 (MQ=255) cttagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGAcgcg > 2:276765‑M2/70‑151 (MQ=255) tagagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGAcgcg < 1:226833‑M2/82‑1 (MQ=255) agagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGt < 1:305845‑M2/84‑1 (MQ=255) agagcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTg < 1:272630‑M2/85‑1 (MQ=255) gcgaggcaccaccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCAt < 1:390220‑M2/88‑1 (MQ=255) caccactttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGtaat > 1:447334‑M2/55‑150 (MQ=255) actttcgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAg < 1:394240‑M2/101‑1 (MQ=255) cgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTtgtg < 2:402944‑M2/106‑1 (MQ=255) cgtaataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTtgtg > 2:120237‑M2/46‑151 (MQ=255) ataccggattcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAgg < 2:92858‑M2/110‑1 (MQ=255) attcgctttccggcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGtt < 1:665113‑M2/117‑1 (MQ=255) gcagtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTAcc > 2:579062‑M2/23‑150 (MQ=255) agtgcgcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAgg > 1:649396‑M2/21‑151 (MQ=255) gcccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTg > 2:334038‑M2/16‑151 (MQ=255) cccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTgg > 1:96054‑M2/15‑151 (MQ=255) cccagaaagcaagtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTgg < 1:513633‑M2/137‑1 (MQ=255) agtGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCg > 2:280927‑M2/4‑150 (MQ=255) | CAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCAACACGCGTTTCCATCACCGGCGTTTCCAGCCCCTGCAATCCAATACCTATTGGATAAACCTGCACAATATTTTCTCCCGGCGGATAATAATAAAGGCGCAGCTCTGCAAGGTTAACGATAATCCCCTGACGCGGTGCATCAGGTAATAACAGTTGTGAAGGAATAGTTATCGTCGTACCAGGTTTTGGCACCGGGGCG > NC_000913/1758218‑1758510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |