| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | NC_000913 | 1,755,661 | Δ2,703 bp | pykF–[ldtE] | pykF, lpp, [ldtE] | |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 1755661 | 1758363 | 2703 | 49 [0] | [1] 50 | pykF–[ldtE] | pykF, lpp, [ldtE] |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 1755660 | 0 (0.000) | 49 (1.140) | 45/296 | 0.1 | 99.0% | intergenic (‑519/‑38) | ydhZ/pykF | uncharacterized protein/pyruvate kinase I |
| ? | NC_000913 | 1758364 = | 1 (0.020) | coding (362/1005 nt) | ldtE | murein L,D‑transpeptidase | |||||
GACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCA > NC_000913/1755515‑1755807 | gACTCAAAAACAGGAACTCACGTTGGGCTGAGACACAAGCAAAAATTCGTATGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGAGCACAGAAAGCAAGTggat > 1:422850‑M1/1‑146 (MQ=255) aCTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggata > 2:50395‑M1/1‑145 (MQ=255) aaCAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgca < 1:19812‑M1/151‑14 (MQ=255) cTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattt > 2:379606‑M1/1‑130 (MQ=255) cACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttc < 2:624600‑M1/151‑24 (MQ=255) cACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatatttt < 2:195941‑M1/150‑23 (MQ=255) cACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattg < 2:421424‑M1/149‑22 (MQ=255) gTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcc < 1:346056‑M1/151‑27 (MQ=255) cgggcTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCggataaacctgcacaatattttctcccg > 2:76296‑M1/2‑123 (MQ=255) gggCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccg < 1:511426‑M1/150‑29 (MQ=255) gCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcg > 1:166377‑M1/1‑120 (MQ=255) tGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcgg > 2:192444‑M1/1‑118 (MQ=255) gagaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcgga < 2:170615‑M1/150‑34 (MQ=255) gagaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcgg < 1:231340‑M1/149‑33 (MQ=255) aGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataa < 1:36034‑M1/151‑43 (MQ=255) gCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaa < 2:452798‑M1/151‑44 (MQ=255) cacacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcgcaatattttctcccggcggataataataaag < 1:538595‑M1/151‑45 (MQ=255) cacacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaag < 2:601592‑M1/151‑45 (MQ=255) cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggc < 1:611173‑M1/151‑47 (MQ=255) caTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgc < 1:138886‑M1/151‑49 (MQ=255) aTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgca < 2:340008‑M1/151‑50 (MQ=255) ttCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgc > 1:543304‑M1/1‑101 (MQ=255) tCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcag < 2:355349‑M1/150‑51 (MQ=255) cGCTTTTTCGATGTCACCTATCCTTAGAGCTAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattctctcccggcggataataataaaggcgcagctctgcaagg < 2:334627‑M1/151‑61 (MQ=255) ttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggtta > 1:6901‑M1/1‑87 (MQ=255) tCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacg > 2:343211‑M1/1‑84 (MQ=255) tGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataat > 2:390209‑M1/1‑80 (MQ=255) gTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataat < 1:577118‑M1/150‑72 (MQ=255) cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatccc > 2:606451‑M1/1‑77 (MQ=255) aCCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccc > 2:587704‑M1/1‑76 (MQ=255) ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccct < 2:190526‑M1/151‑77 (MQ=255) cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcg > 2:276765‑M1/1‑69 (MQ=255) cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcg > 1:424115‑M1/1‑69 (MQ=255) tAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcg < 1:226833‑M1/149‑83 (MQ=255) agagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtg < 1:272630‑M1/151‑86 (MQ=255) agagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggt < 1:305845‑M1/150‑85 (MQ=255) gCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcat < 1:390220‑M1/151‑89 (MQ=255) caccacTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaat > 1:447334‑M1/1‑54 (MQ=255) acTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacag < 1:394240‑M1/151‑102 (MQ=255) cGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtg > 2:120237‑M1/1‑45 (MQ=255) cGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtg < 2:402944‑M1/151‑107 (MQ=255) aTACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaagg < 2:92858‑M1/151‑111 (MQ=255) aTTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagtt < 1:665113‑M1/151‑118 (MQ=255) gCAGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagttatcgtcgtacc > 2:579062‑M1/1‑22 (MQ=255) aGTGCGCCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagttatcgtcgtaccagg > 1:649396‑M1/1‑20 (MQ=255) gcCCAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagttatcgtcgtaccaggttttg > 2:334038‑M1/1‑15 (MQ=255) cccAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagttatcgtcgtaccaggttttgg > 1:96054‑M1/1‑14 (MQ=255) cccAGAAAGCAAGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagttatcgtcgtaccaggttttgg < 1:513633‑M1/151‑138 (MQ=255) aGTggataaacctgcacaatattttctcccggcggataataataaaggcgcagctctgcaaggttaacgataatcccctgacgcggtgcatcaggtaataacagttgtgaaggaatagttatcgtcgtaccaggttttggcaccggggcg > 2:280927‑M1/1‑3 (MQ=255) | GACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCA > NC_000913/1755515‑1755807 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |