Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	699,916	A→G	G137G (GGT→GGC)	umpH ←	UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	699,916	0	A	G	100.0%	106.1 / NA	31	G137G (GGT→GGC)	umpH	UMP phosphatase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/14); total (17/14)

ATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCAC  >  NC_000913/699771‑700062

aTGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGtt                                                                                                                                               >  1:518997/1‑151 (MQ=255)

     gcgcGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGcg                                                                                                                                           <  2:153964/150‑1 (MQ=255)

            tgatCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCTGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAAt                                                                                                                                    >  1:15270/1‑150 (MQ=255)

                ccACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGgct                                                                                                                               <  2:569821/151‑1 (MQ=255)

                 cACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGgctg                                                                                                                              >  1:189779/1‑151 (MQ=255)

                 cACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGgctg                                                                                                                              <  2:541680/151‑1 (MQ=255)

                                         aaCGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGt                                                                                                      >  1:378044/1‑151 (MQ=255)

                                                     ccGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGttt                                                                                          <  1:323986/151‑1 (MQ=255)

                                                     ccGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGttt                                                                                          >  1:99815/1‑151 (MQ=255)

                                                       ggAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTc                                                                                         <  2:16078/150‑1 (MQ=255)

                                                           aTTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCca                                                                                     >  1:409698/1‑150 (MQ=255)

                                                              ttCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCcaaca                                                                                  <  1:575345/150‑1 (MQ=255)

                                                                  cAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCa                                                                              <  2:63006/150‑1 (MQ=255)

                                                                  cAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCa                                                                              <  2:619869/150‑1 (MQ=255)

                                                                    aTCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACg                                                                            >  1:587014/1‑150 (MQ=255)

                                                                     tCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGa                                                                           >  1:68206/1‑150 (MQ=255)

                                                                     tCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGa                                                                           >  1:231863/1‑150 (MQ=255)

                                                                            acacaACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAggg                                                                   >  1:483145/1‑151 (MQ=255)

                                                                            acacaACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAggg                                                                   <  1:525930/151‑1 (MQ=255)

                                                                                 aCGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTcac                                                              >  1:201770/1‑151 (MQ=255)

                                                                                 aCGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTcac                                                              >  1:281813/1‑151 (MQ=255)

                                                                                             cGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGt                                                  >  2:253501/1‑151 (MQ=255)

                                                                                                   aaaaaCCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAAcc                                            >  2:358651/1‑151 (MQ=255)

                                                                                                                gTGGGTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGt                               <  1:434503/151‑1 (MQ=255)

                                                                                                                   ggTGTCCGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCa                            <  2:144945/151‑1 (MQ=255)

                                                                                                                        ccGGATTGGTGGCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAAt                       <  2:444714/151‑1 (MQ=255)

                                                                                                                                   gCGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCtt            <  1:42590/151‑1 (MQ=255)

                                                                                                                                    cGATAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAACCCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTc           <  2:498269/151‑1 (MQ=255)

                                                                                                                                      aTAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGc         >  2:450183/1‑151 (MQ=255)

                                                                                                                                       tAAAACGTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGcc        >  1:462444/1‑151 (MQ=255)

                                                                                                                                             gTGCGCCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCcaccac  >  2:317670/1‑151 (MQ=255)

ATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCAC  >  NC_000913/699771‑700062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A