Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	838,311	+G	coding (146/267 nt)	ybiI ←	DksA‑type zinc finger protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	838,308	1	.	G	97.4%	132.7 / ‑4.0	39	coding (149/267 nt)	ybiI	DksA‑type zinc finger protein
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (18/20); total (19/20)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATACTCGCTATGCATCTCGCCAGTTGTAGTTAACGTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGAC‑GGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGA  >  NC_000913/838161‑838450

ataCTCGCTATGCATCTCGCCAGTTGTAGTTAACGTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGAC‑ggg                                                                                                                                             <  1:612607/151‑1 (MQ=255)

  aCTCGCTATGCATCTCGCCAGTTGTAGTTAACGTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGAC‑ggggc                                                                                                                                           >  1:651076/1‑149 (MQ=255)

   cTCGCTATGCATCTCGCCAGTTGTAGTTAACGTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGAC‑ggggcc                                                                                                                                          >  2:608798/1‑148 (MQ=255)

   cTCGCTATGCATCTCGCCAGTTGTAGTTAACGTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGAC‑ggggcc                                                                                                                                          >  2:111436/1‑148 (MQ=255)

    tCGCTATGCATCTCGCCAGATGTAGTTAACGTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGAC‑ggggcct                                                                                                                                         >  2:535136/1‑147 (MQ=255)

        tATGCATCTCGCCAGTTGTAGTTAACGTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCgg                                                                                                                                      >  1:671902/1‑151 (MQ=255)

         aTGCATCTCGCCAGTTGTAGTTAACGTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCg                                                                                                                                       <  2:87188/149‑1 (MQ=255)

           gCATCTCGCCAGTTGTAGTTAACGTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGAt                                                                                                                                   <  1:208309/151‑1 (MQ=255)

                 cGCCAGTTGTAGTTAACGTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGc                                                                                                                             >  2:30446/1‑151 (MQ=255)

                           aGTTAACGTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCActct                                                                                                                   <  1:92147/151‑1 (MQ=255)

                                  gTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACAtt                                                                                                            >  2:82991/1‑151 (MQ=255)

                                  gTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACAtt                                                                                                            >  2:23597/1‑151 (MQ=255)

                                         gCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAg                                                                                                     <  1:185957/151‑1 (MQ=255)

                                           tGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGc                                                                                                   >  2:211254/1‑151 (MQ=255)

                                              cTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCttt                                                                                                >  1:671678/1‑151 (MQ=255)

                                                  cGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTcgcc                                                                                            >  1:660928/1‑151 (MQ=255)

                                                  cGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTcgc                                                                                             <  2:612921/150‑1 (MQ=255)

                                                           gCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAAt                                                                                   <  2:398488/151‑1 (MQ=255)

                                                             gATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAAttt                                                                                 <  1:240668/151‑1 (MQ=255)

                                                             gATTATATCCTATATAAGCTGGTTTTTGTAAAACTTTCTCCTGCTGACAATGAATACATAAGCGCACTCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGa                                                                                     <  2:386080/147‑1 (MQ=255)

                                                                 atatCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCAcc                                                                             >  2:566364/1‑151 (MQ=255)

                                                                 atatCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCAc                                                                              <  1:246075/150‑1 (MQ=255)

                                                                     ccTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCg                                                                          >  2:581477/1‑150 (MQ=255)

                                                                        gTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAgc                                                                       <  1:188637/150‑1 (MQ=255)

                                                                            aaGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCggg                                                                  <  1:34043/151‑1 (MQ=255)

                                                                                       tGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCt                                                       >  1:288640/1‑151 (MQ=255)

                                                                                         tAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTc                                                     >  2:262209/1‑151 (MQ=255)

                                                                                          aaaTCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTc                                                     <  1:19643/150‑1 (MQ=255)

                                                                                                ttCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGt                                               <  1:328622/150‑1 (MQ=255)

                                                                                                     ctgctgACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTGCTGtt                                         >  1:500519/1‑151 (MQ=255)

                                                                                                        ctgACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCTCTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGa                                       <  2:249824/150‑1 (MQ=255)

                                                                                                        ctgACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGAt                                      >  2:596571/1‑151 (MQ=255)

                                                                                                                    aCATAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGa                           <  1:111438/150‑1 (MQ=255)

                                                                                                                       tAAGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGc                       >  1:563391/1‑151 (MQ=255)

                                                                                                                        aaGCGCACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCg                      <  1:597818/151‑1 (MQ=255)

                                                                                                                          gcgcACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTc                    >  1:663277/1‑151 (MQ=255)

                                                                                                                           cgcACGCCAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCa                   <  2:234970/151‑1 (MQ=255)

                                                                                                                                 ccAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTa             <  1:632419/151‑1 (MQ=255)

                                                                                                                                  cAGGAATGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGtt              >  2:564219/1‑149 (MQ=255)

                                                                                                                                  cAGGAATGGCTTCCCGAC‑GGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGc           >  2:211096/1‑151 (MQ=255)

                                                                                                                                       aTGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCaa       <  1:580898/151‑1 (MQ=255)

                                                                                                                                        tGGCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAAc      >  2:91106/1‑151 (MQ=255)

                                                                                                                                         ggCTTCCCGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAAcc     >  2:247896/1‑151 (MQ=255)

                                                                                                                                               ccGACGGGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCACGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGa  <  1:127225/148‑1 (MQ=255)

ATACTCGCTATGCATCTCGCCAGTTGTAGTTAACGTAACTGGCTGTCTTTCGAACCTCTGCGATTATATCCTGTATAAGCTGGTTTTTGTAAATCTTTCTCCTGCTGACAATGAATACATAAGCGCACGCCAGGAATGGCTTCCCGAC‑GGGCCTGCGGGATGGGGGCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGA  >  NC_000913/838161‑838450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A