Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	867,112	C→G	A182G (GCC→GGC)	iaaA →	Isoaspartyl peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	867,112	0	C	G	100.0%	169.7 / NA	47	A182G (GCC→GGC)	iaaA	Isoaspartyl peptidase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (33/14); total (33/14)

CTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCCAATAACGCCAGTGTGGCGGTTTCTTGTAC  >  NC_000913/866965‑867256

cTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCg                                                                                                                                                >  1:347435/1‑150 (MQ=255)

     ggCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGggcgtggcgt                                                                                                                                          <  1:414087/151‑1 (MQ=255)

              cgcgTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAg                                                                                                                                 <  2:139834/151‑1 (MQ=255)

                     cGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCa                                                                                                                           >  2:342463/1‑150 (MQ=255)

                     cGCCGGAGATTTTCTCCACATCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCa                                                                                                                           <  1:428805/150‑1 (MQ=255)

                       ccGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAAtt                                                                                                                        >  2:398931/1‑151 (MQ=255)

                       ccGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAAtt                                                                                                                        <  1:372951/151‑1 (MQ=255)

                         ggAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTg                                                                                                                      <  2:51279/151‑1 (MQ=255)

                          gagaTTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTgg                                                                                                                     <  1:91071/151‑1 (MQ=255)

                               tttCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTggcggc                                                                                                                 >  2:129427/1‑150 (MQ=255)

                                ttCTCCACGTCTTTGCGCTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTggcggc                                                                                                                 >  2:410628/1‑149 (MQ=255)

                                        gTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTcc                                                                                                       >  2:60608/1‑151 (MQ=255)

                                              gCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGc                                                                                                 >  1:348681/1‑151 (MQ=255)

                                                 ttATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCgg                                                                                               >  1:369164/1‑150 (MQ=255)

                                                  tATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGa                                                                                              >  2:556655/1‑150 (MQ=255)

                                                   aTGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAAt                                                                                            >  2:344372/1‑151 (MQ=255)

                                                         aacTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCa                                                                                       >  2:375843/1‑150 (MQ=255)

                                                         aacTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCa                                                                                       >  2:288657/1‑150 (MQ=255)

                                                            tactGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATa                                                                                    >  1:432902/1‑150 (MQ=255)

                                                              ctGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAAt                                                                                 >  2:275268/1‑151 (MQ=255)

                                                                ggCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAAtt                                                                                >  1:536664/1‑150 (MQ=255)

                                                                   aGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTAcgc                                                                            <  2:142542/151‑3 (MQ=255)

                                                                       cgcAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGAc                                                                        <  1:177071/151‑1 (MQ=255)

                                                                        gcAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACg                                                                       >  1:380791/1‑151 (MQ=255)

                                                                          aaggaaggGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACg                                                                       >  2:183380/1‑149 (MQ=255)

                                                                          aaggaaggGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACg                                                                       >  1:554836/1‑149 (MQ=255)

                                                                            ggaaggGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGtt                                                                   <  1:313522/151‑1 (MQ=255)

                                                                                   gCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATa                                                            <  1:570828/151‑1 (MQ=255)

                                                                                    cAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGAt                                                             >  1:63446/1‑149 (MQ=255)

                                                                                         gTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCt                                                      >  1:358359/1‑151 (MQ=255)

                                                                                            cTCGACCATAGCGGTGCGCCATTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCtt                                                     >  2:589764/1‑149 (MQ=255)

                                                                                                aCCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTggg                                               >  1:239810/1‑151 (MQ=255)

                                                                                                       cGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGa                                        <  2:259975/151‑1 (MQ=255)

                                                                                                        ggTGCGCCACTGGGTGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGAt                                       <  1:505625/151‑1 (MQ=255)

                                                                                                          tGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGc                                     >  2:530910/1‑151 (MQ=255)

                                                                                                             gcCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTAc                                  >  1:127512/1‑151 (MQ=255)

                                                                                                                aCTGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGcc                               >  1:612796/1‑151 (MQ=255)

                                                                                                                  tGGATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCCa                              >  2:62135/1‑150 (MQ=255)

                                                                                                                   ggATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCCAAt                            <  1:239456/151‑1 (MQ=255)

                                                                                                                    gATGAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCCAAt                            >  1:573213/1‑150 (MQ=255)

                                                                                                                       gAAAAACAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCCAATAACg                        >  1:12104/1‑151 (MQ=255)

                                                                                                                           aaCAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCCAATAACGcc                      >  1:104789/1‑149 (MQ=255)

                                                                                                                             cAGAAAATGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCCAATAACGCCAgtg                  >  1:251378/1‑151 (MQ=255)

                                                                                                                                aaaaTGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCCAATAACGCCAGTGTgg               >  1:577450/1‑151 (MQ=255)

                                                                                                                                aaaaTGGGCACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGAGCCGGATGCTACGCCAATAACGCCAGTGTgg               >  1:267836/1‑151 (MQ=255)

                                                                                                                                        cACCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCCAATAACGCCAGTGTGGCGGTTTCt       <  2:463599/151‑1 (MQ=255)

                                                                                                                                         aCCGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCCAATAACGCCAGTGTGGCGGTTTCtt      >  1:603485/1‑151 (MQ=255)

                                                                                                                                          ccGTGGGGGGCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCCAATAACGCCAGTGTGGCGGTTTCTTg     <  2:502027/151‑1 (MQ=255)

                                                                                                                                              ggggggCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCCAATAACGCCAGTGTGGCGGTTTCTTGTAc  <  2:127509/150‑1 (MQ=255)

CTCGTGGCATGGAGCGCGTCTCGCCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGGGCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCCAATAACGCCAGTGTGGCGGTTTCTTGTAC  >  NC_000913/866965‑867256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A