Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	958,916	A→G	A35A (GCA→GCG)	aroA →	5‑enolpyruvylshikimate‑3‑phosphate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	958,916	0	A	G	100.0%	158.3 / NA	45	A35A (GCA→GCG)	aroA	5‑enolpyruvylshikimate‑3‑phosphate synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/26); total (19/26)

GCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCAGCCGATCGTACGCGTTGCGAAATTATCGGTAACGGCGGTCCATTACAC  >  NC_000913/958772‑959060

gccTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGc                                                                                                                                            >  1:569932/1‑151 (MQ=255)

    gtgGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCaaa                                                                                                                                         >  1:261766/1‑150 (MQ=255)

      ggggTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAAc                                                                                                                                       <  2:231821/150‑1 (MQ=255)

         gTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTa                                                                                                                                   <  2:512493/151‑1 (MQ=255)

              tATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTaa                                                                                                                               <  2:105417/150‑1 (MQ=255)

                    tgttgtAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATct                                                                                                                        >  2:492371/1‑151 (MQ=255)

                     gttgtAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATct                                                                                                                        <  2:116362/150‑1 (MQ=255)

                       tgtAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATctgc                                                                                                                      <  1:272418/150‑1 (MQ=255)

                        gtAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATctgctg                                                                                                                    <  2:332730/151‑1 (MQ=255)

                            agagTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATa                                                                                                                <  1:488381/151‑1 (MQ=255)

                                     ttCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACg                                                                                                       >  2:486659/1‑151 (MQ=255)

                                       cATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTg                                                                                                     <  2:309716/151‑1 (MQ=255)

                                        aTGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTgc                                                                                                    <  1:80404/151‑1 (MQ=255)

                                        aTGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTgc                                                                                                    >  1:60086/1‑151 (MQ=255)

                                         tGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAAACGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTgcg                                                                                                   >  1:116149/1‑151 (MQ=255)

                                               cccTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCatat                                                                                             >  1:228886/1‑151 (MQ=255)

                                               cccTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCatat                                                                                             >  2:528117/1‑151 (MQ=255)

                                                     cGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGaa                                                                                       <  1:69951/151‑1 (MQ=255)

                                                      gTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAAt                                                                                      <  2:141118/151‑1 (MQ=255)

                                                        tACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATg                                                                                     <  1:75443/150‑1 (MQ=255)

                                                          cAACCCATCGCTCGTGTCGATGGCTCTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACTGCAAAACGCTATTAACCAATCTGCTGGATAGCTATGACGTGCGTCATATGCTGAATGc                                                                                    <  2:444623/149‑1 (MQ=255)

                                                            aCCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCAtt                                                                                 >  2:283041/1‑150 (MQ=255)

                                                             cccATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTaa                                                                               <  1:461303/151‑1 (MQ=255)

                                                             cccATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTaa                                                                               <  2:186217/151‑1 (MQ=255)

                                                                aTCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAAc                                                                              >  2:123820/1‑149 (MQ=255)

                                                                   gCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGt                                                                         >  1:139448/1‑151 (MQ=255)

                                                                         gtCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAgggg                                                                   >  2:534024/1‑151 (MQ=255)

                                                                         gtCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAgggg                                                                   >  1:495495/1‑151 (MQ=255)

                                                                          tCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGt                                                                  >  2:334784/1‑151 (MQ=255)

                                                                            gATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTa                                                                 >  1:34898/1‑150 (MQ=255)

                                                                                       tAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCtt                                                     >  2:449594/1‑151 (MQ=255)

                                                                                        aaTCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCtt                                                     <  2:88723/150‑1 (MQ=255)

                                                                                             gCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTc                                                   <  1:305557/147‑1 (MQ=255)

                                                                                             gCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCa                                                  <  2:12600/148‑1 (MQ=255)

                                                                                             gCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCAGcc                                               <  1:312555/151‑1 (MQ=255)

                                                                                             gCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCAGc                                                <  1:202434/150‑1 (MQ=255)

                                                                                                  gTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCAGCCGATCg                                          <  2:573060/151‑1 (MQ=255)

                                                                                                           gCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCAGCCGATCGTACGCGtt                                  <  1:257864/150‑1 (MQ=255)

                                                                                                                    aCCGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCAGCCGATCGTACGCGTTGCGAAATTAt                        <  2:105829/151‑1 (MQ=255)

                                                                                                                     ccGCGCTTTATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCAGCCGATCGTACGCGTTGCGAAATTATc                       <  1:505545/151‑1 (MQ=255)

                                                                                                                             tATTGCTGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCAGCCGATCGTACGCGTTGCGAAATTATCGGTAAcg                <  2:619841/150‑1 (MQ=255)

                                                                                                                                   tGGCGGCATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCAGCCGATCGTACGCGTTGCGAAATTATCGGTAACGGCGGTcc         >  2:274669/1‑151 (MQ=255)

                                                                                                                                       ggcATTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCAGCCGATCGTACGCGTTGCGAAATTATCGGTAACGGCGGTCCATTa     <  2:407605/151‑1 (MQ=255)

                                                                                                                                          aTTAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCAGCCGATCGTACGCGTTGCGAAATTATCGGTAACGGCGGTCCATTac    >  2:134337/1‑149 (MQ=255)

                                                                                                                                           ttAGCGCACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCAGCCGATCGTACGCGTTGCGAAATTATCGGTAACGGCGGTCCATTacac  >  2:490610/1‑150 (MQ=255)

GCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACACGGCAAAACAGTATTAACCAATCTGCTGGATAGCGATGACGTGCGCCATATGCTGAATGCATTAACAGCGTTAGGGGTAAGCTATACGCTTTCAGCCGATCGTACGCGTTGCGAAATTATCGGTAACGGCGGTCCATTACAC  >  NC_000913/958772‑959060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A