Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,916,069	T→C	G20G (GGA→GGG)	msrC ←	free methionine‑(R)‑sulfoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,916,069	0	T	C	100.0%	144.8 / NA	41	G20G (GGA→GGG)	msrC	free methionine‑(R)‑sulfoxide reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/27); total (14/27)

TATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCCCATCA  >  NC_000913/1915922‑1916215

tATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCcccc                                                                                                                                                 >  2:106041/1‑151 (MQ=255)

 aTCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCcccc                                                                                                                                                 >  1:453822/1‑150 (MQ=255)

 aTCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCcccc                                                                                                                                                 <  2:9345/150‑1 (MQ=255)

      gACACAGGCAATTTTGCCCTGAAATGGTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATc                                                                                                                                           <  1:269276/151‑1 (MQ=255)

       acacAGGCAATTTTGCCCTGAAATGGTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAACCCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCa                                                                                                                                          <  2:123357/151‑1 (MQ=255)

               aaTTTTGCCCTGAAATGGTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTa                                                                                                                                  <  1:290674/151‑1 (MQ=255)

                  tttGCCCTGAAATGGTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTaa                                                                                                                                 >  1:346409/1‑149 (MQ=255)

                   ttGCCCTGAAATGGTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAg                                                                                                                               <  1:572241/150‑1 (MQ=255)

                           aaaTGGTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGAttt                                                                                                                      >  1:546802/1‑151 (MQ=255)

                                gTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATc                                                                                                                 <  1:120903/151‑1 (MQ=255)

                                   cGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCcgc                                                                                                              >  1:489597/1‑151 (MQ=255)

                                    gAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCcgcg                                                                                                             >  1:605826/1‑151 (MQ=255)

                                       tACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTa                                                                                                           >  2:52972/1‑150 (MQ=255)

                                         ccAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTaaa                                                                                                         >  1:275202/1‑150 (MQ=255)

                                            gtgtATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAAtt                                                                                                      <  1:592797/150‑1 (MQ=255)

                                                  cGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGtttt                                                                                               <  2:619973/151‑1 (MQ=255)

                                                        cAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCAt                                                                                         <  1:84250/151‑1 (MQ=255)

                                                          aGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATta                                                                                       <  2:571026/151‑1 (MQ=255)

                                                          aGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATta                                                                                       <  1:51502/151‑1 (MQ=255)

                                                                 aaaaaCCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAAt                                                                                 <  1:378182/150‑1 (MQ=255)

                                                                   aaaCCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCAc                                                                              >  2:552830/1‑151 (MQ=255)

                                                                         gCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTgg                                                                          <  2:67280/149‑1 (MQ=255)

                                                                          cccAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTg                                                                       <  2:630968/151‑1 (MQ=255)

                                                                          cccAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTg                                                                       <  1:667861/151‑1 (MQ=255)

                                                                               tttATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCtt                                                                   >  2:404409/1‑150 (MQ=255)

                                                                                ttATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTAc                                                                 >  1:388550/1‑151 (MQ=255)

                                                                                         tGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTg                                                        >  1:146440/1‑151 (MQ=255)

                                                                                              cGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAg                                                    <  1:125421/150‑1 (MQ=255)

                                                                                                cTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCt                                                 >  2:423977/1‑151 (MQ=255)

                                                                                                  cATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTa                                                <  2:369700/150‑1 (MQ=255)

                                                                                                  cATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTAt                                               <  1:649511/151‑1 (MQ=255)

                                                                                                  cATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTAt                                               <  1:279897/151‑1 (MQ=255)

                                                                                                   atatAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTAt                                               <  2:9188/150‑1 (MQ=255)

                                                                                                     ataACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTa                                             <  1:409731/150‑1 (MQ=255)

                                                                                                            cGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGc                                     <  1:332344/151‑1 (MQ=255)

                                                                                                             gCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCa                                    <  2:533143/151‑1 (MQ=255)

                                                                                                              cACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCAt                                   <  2:557455/151‑1 (MQ=255)

                                                                                                                          cAAGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTa                        >  2:321133/1‑150 (MQ=255)

                                                                                                                           aaGCGTTGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAAt                      >  1:321233/1‑151 (MQ=255)

                                                                                                                                ttGCCAGAAAACTGGTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTc                  <  2:621703/150‑1 (MQ=255)

                                                                                                                                    cAGAAAACTGGTTTCCCCCGCCATCAGCGCGTCAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGAt             <  1:70259/151‑1 (MQ=255)

                                                                                                                                             ggTTTCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTccc      <  1:209745/149‑1 (MQ=255)

                                                                                                                                               tttCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCCcatc   >  1:639767/1‑150 (MQ=255)

                                                                                                                                                ttCCCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCCcatca  <  1:523476/150‑1 (MQ=255)

TATCCGGACACAGGCAATTTTGCCCTGAAATGGTCCGAGTACCAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCCCATCA  >  NC_000913/1915922‑1916215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A