Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,258,212	T→C	E52E (GAA→GAG)	psuG ←	pseudouridine 5'‑phosphate glycosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,258,212	0	T	C	100.0%	126.7 / NA	36	E52E (GAA→GAG)	psuG	pseudouridine 5'‑phosphate glycosidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/18); total (18/18)

AACTTTGGTCACGTTATGCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTT  >  NC_000913/2258086‑2258355

aaCTTTGGTCACGTTATGCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTggg                                                                                                                           <  1:276031/149‑1 (MQ=255)

aaCTTTGGTCACGTTATGCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGc                                                                                                                          <  1:153198/150‑1 (MQ=255)

   tttGGTCACGTTATGCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGGCTGGGCAttt                                                                                                                      >  2:372370/1‑151 (MQ=255)

         cACGTTATGCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTggg                                                                                                                <  2:379426/151‑1 (MQ=255)

           cGTTATGCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGa                                                                                                               <  1:577552/150‑1 (MQ=255)

                         cGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAACCCCCTGTTTACGAATAGGTTCCTCAACTTCAATTGCGGTCTGGGCCTTTTTTGGGGACGGCATccc                                                                                                     >  2:2421/1‑146 (MQ=255)

                                      cAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCg                                                                                    <  2:65640/150‑1 (MQ=255)

                                        aTTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGGACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCCTTTTGTGGGAACGGCATCCCGTGGGAAATAATGGTCGAtt                                                                                 >  2:549802/1‑151 (MQ=255)

                                           tcttctTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGGTTACAAAGAGATTCCTCAACTTCACTTGCGGTCTGGGCATTTTGTGGGAACGGGATCCCGTGAGAAATAATGGGCGATTcc                                                                               >  1:269450/1‑150 (MQ=255)

                                           tcttctTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAATGCCCCGTTTACGAATAGGTTTCTCAACTTCCATTGCGGTCTGGGCATTTTTTGGGAAAGGCATCCCGTGAGAAATAATGGTCGCtt                                                                                 >  2:390647/1‑148 (MQ=255)

                                                 ttGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGGACAGCGCCCTGTTTACGAATAATTTCCTCAACTTCAATTGGGGTCTGGGGATTTTGGGGGAACGGCATCCCGTGAGAAATAATCGGCGATTCCAGCGcc                                                                         >  1:336727/1‑150 (MQ=255)

                                                   gCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCcaca                                                                      <  2:215968/151‑1 (MQ=255)

                                                         ccccACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCCGTTTACGAATAGTTTCCTCAAATTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCCCAACCgg                                                                 >  1:491190/1‑150 (MQ=255)

                                                          cccACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGGCTGGGCCTTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCCCAACCgg                                                                 >  2:409837/1‑149 (MQ=255)

                                                           ccACTTTCATCACGCCGCCGATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAAGTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgtt                                                               <  2:97104/150‑1 (MQ=255)

                                                              cTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCCCAACCGggttttt                                                           >  1:140209/1‑151 (MQ=255)

                                                               tttCATCACGCCGCCAATAATGGCGATCGTGGCAGGGACAGCGCCCTGTTTACGAATAGTTTTCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgtttttt                                                           >  1:662919/1‑150 (MQ=255)

                                                                ttCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttg                                                          >  1:97129/1‑150 (MQ=255)

                                                                ttCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttg                                                          <  2:164371/150‑1 (MQ=255)

                                                                     caCGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttgttttt                                                     <  2:3737/150‑1 (MQ=255)

                                                                       cgccgcCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTaa                                                  <  1:5576/151‑1 (MQ=255)

                                                                        gccgccAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttgtttttt                                                    >  2:560222/1‑148 (MQ=255)

                                                                            ccAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGt                                             <  1:19045/151‑1 (MQ=255)

                                                                                      cGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTc                                   <  2:618661/151‑1 (MQ=255)

                                                                                            tGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCgggg                              >  2:116813/1‑150 (MQ=255)

                                                                                              gCAGGTACAGCGCCCTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGaaa                           <  2:615758/151‑1 (MQ=255)

                                                                                                          cccTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAAtt               <  2:354584/151‑1 (MQ=255)

                                                                                                           ccTGTTTACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTc              <  1:559116/151‑1 (MQ=255)

                                                                                                               tttACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAggg          >  1:267999/1‑151 (MQ=255)

                                                                                                               tttACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAggg          >  1:601541/1‑151 (MQ=255)

                                                                                                                ttACGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAgggg         >  2:558983/1‑151 (MQ=255)

                                                                                                                  aCGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGaa       <  1:409848/151‑1 (MQ=255)

                                                                                                                  aCGAATAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGa        >  1:595671/1‑150 (MQ=255)

                                                                                                                     aaTAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAAtt    <  1:525539/151‑1 (MQ=255)

                                                                                                                       tAGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAAtttt  >  2:566294/1‑151 (MQ=255)

                                                                                                                        aGTTTCCTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAAtttt  <  2:618122/150‑1 (MQ=255)

AACTTTGGTCACGTTATGCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTT  >  NC_000913/2258086‑2258355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A