Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I2 R1 150 68.2 2132745 98.7% 2105019 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,787,381 A→G intergenic (‑269/‑64) ppsA ← / → ppsR phosphoenolpyruvate synthase/PEP synthase kinase and PEP synthase pyrophosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,787,3810AG98.5% 242.6 / NA 65intergenic (‑269/‑64)ppsA/ppsRphosphoenolpyruvate synthase/PEP synthase kinase and PEP synthase pyrophosphorylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (41/23);  minor base C (0/1);  total (41/24)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAAGACTGATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAAT  >  NC_000913/1787233‑1787527
                                                                                                                                                    |                                                                                                                                                  
tttAAGACTGATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCg                                                                                                                                                  >  1:1059792/1‑151 (MQ=255)
tttAAGACTGATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCg                                                                                                                                                  >  1:535767/1‑151 (MQ=255)
   aaGACTGATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCtt                                                                                                                                               >  1:1218/1‑151 (MQ=255)
    aGACTGATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTc                                                                                                                                              <  1:962470/151‑1 (MQ=255)
     gACTGATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCt                                                                                                                                             <  2:853612/151‑1 (MQ=255)
         gATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGc                                                                                                                                         >  2:962672/1‑151 (MQ=255)
           tCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGt                                                                                                                                       >  1:44734/1‑151 (MQ=255)
            cccAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGtt                                                                                                                                      >  2:245099/1‑151 (MQ=255)
            cccAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGtt                                                                                                                                      >  1:421906/1‑151 (MQ=255)
             ccAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCGCATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGttt                                                                                                                                     <  2:395147/151‑1 (MQ=255)
               aGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGttttt                                                                                                                                   >  2:428987/1‑151 (MQ=255)
               aGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGttttt                                                                                                                                   >  1:687017/1‑151 (MQ=255)
               aGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGttttt                                                                                                                                   <  1:522511/151‑1 (MQ=255)
                 cGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTa                                                                                                                                  >  1:399988/1‑150 (MQ=255)
                  gTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAAt                                                                                                                                <  1:948298/151‑1 (MQ=255)
                   ttGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAATCCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATATTATAAACCAGACATAACGCTCCCCGCTTCTTTGCGTTTTTaa                                                                                                                                 <  2:862641/149‑1 (MQ=255)
                    tGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAAtt                                                                                                                               <  1:407424/150‑1 (MQ=255)
                          aaTCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCtt                                                                                                                        >  2:912068/1‑151 (MQ=255)
                                       cGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATg                                                                                                           >  2:934659/1‑151 (MQ=255)
                                       cGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATg                                                                                                           <  1:1061791/150‑1 (MQ=255)
                                                   caTAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACgg                                                                                               <  2:285860/151‑1 (MQ=255)
                                                   caTAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACgg                                                                                               >  2:847825/1‑151 (MQ=255)
                                                        aCCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGga                                                                                             >  1:923279/1‑148 (MQ=255)
                                                         ccATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGgaaga                                                                                          >  2:791525/1‑150 (MQ=255)
                                                               gTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAAt                                                                                   <  1:654336/151‑1 (MQ=255)
                                                                tataAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAAt                                                                                   <  1:689507/150‑1 (MQ=255)
                                                                tataAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATg                                                                                  >  2:638868/1‑151 (MQ=255)
                                                                tataAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATg                                                                                  >  2:352049/1‑151 (MQ=255)
                                                                   aaaCGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGAt                                                                               <  2:515778/151‑1 (MQ=255)
                                                                    aaCGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGAt                                                                               >  2:842206/1‑150 (MQ=255)
                                                                     aCGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATa                                                                              >  1:345854/1‑150 (MQ=255)
                                                                       gAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATg                                                                           >  1:489859/1‑151 (MQ=255)
                                                                           ccATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGt                                                                       >  2:535265/1‑151 (MQ=255)
                                                                            cATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGtt                                                                      >  1:918500/1‑151 (MQ=255)
                                                                             aTAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTg                                                                     <  1:308191/151‑1 (MQ=255)
                                                                                   atgaaatgCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGcc                                                               >  1:207182/1‑151 (MQ=255)
                                                                                     gaaatgCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCAc                                                             >  2:727403/1‑151 (MQ=255)
                                                                                      aaatgCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACg                                                            <  1:971677/151‑1 (MQ=255)
                                                                                      aaatgCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACg                                                            >  2:1007098/1‑151 (MQ=255)
                                                                                          gCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGttt                                                         <  1:447168/150‑1 (MQ=255)
                                                                                           cTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGttttt                                                       >  2:1032540/1‑151 (MQ=255)
                                                                                           cTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGttttt                                                       >  1:593996/1‑151 (MQ=255)
                                                                                                ttCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTtata                                                   <  1:900168/150‑1 (MQ=255)
                                                                                                ttCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTatat                                                  >  1:61669/1‑151 (MQ=255)
                                                                                                  cATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATAtt                                                 <  1:668208/150‑1 (MQ=255)
                                                                                                      aaaaaTAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTg                                             >  2:534499/1‑150 (MQ=255)
                                                                                                       aaaataaaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACTGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGAt                                           <  2:93838/151‑1 (MQ=255)
                                                                                                         aataaaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATgg                                         >  1:849210/1‑151 (MQ=255)
                                                                                                         aataaaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATgg                                         >  1:878715/1‑151 (MQ=255)
                                                                                                            aaaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTAc                                      >  2:1001277/1‑151 (MQ=255)
                                                                                                             aaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACg                                     <  1:83615/151‑1 (MQ=255)
                                                                                                               atTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGc                                   >  2:923267/1‑151 (MQ=255)
                                                                                                                 tGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCa                                  <  1:955412/150‑1 (MQ=255)
                                                                                                                  gAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAAt                                <  1:867097/151‑1 (MQ=255)
                                                                                                                   aaGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAAt                                >  2:824426/1‑150 (MQ=255)
                                                                                                                         cATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCg                         >  1:947129/1‑151 (MQ=255)
                                                                                                                         cATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCg                         >  2:50761/1‑151 (MQ=255)
                                                                                                                         cATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCg                         >  2:408408/1‑151 (MQ=255)
                                                                                                                            tttATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGa                       <  1:680216/150‑1 (MQ=255)
                                                                                                                                        aCATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGaca           >  1:479565/1‑150 (MQ=255)
                                                                                                                                         cATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACg         >  1:348581/1‑151 (MQ=255)
                                                                                                                                             aCGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGt     <  1:265301/151‑1 (MQ=255)
                                                                                                                                             aCGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGt     <  2:308692/151‑1 (MQ=255)
                                                                                                                                               gTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTa    >  2:58193/1‑150 (MQ=255)
                                                                                                                                                ttCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGGCTGCATCACGGGAGGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAAt  >  2:51779/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTTAAGACTGATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAAT  >  NC_000913/1787233‑1787527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A