Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F50 I1 R1
|
163 |
55.0 |
1534755 |
98.6% |
1513268 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,787,381 |
A→G |
intergenic (‑269/‑64) |
ppsA ← / → ppsR |
phosphoenolpyruvate synthase/PEP synthase kinase and PEP synthase pyrophosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,787,381 | 0 | A | G | 100.0%
| 34.3
/ NA
| 10 | intergenic (‑269/‑64) | ppsA/ppsR | phosphoenolpyruvate synthase/PEP synthase kinase and PEP synthase pyrophosphorylase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (9/1); total (9/1) |
TAAGACTGATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATT > NC_000913/1787235‑1787512
|
tAAGACTGATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCt > 2:150463/1‑151 (MQ=255)
aTTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGc > 2:15846/1‑151 (MQ=255)
ttttCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTtat > 2:201750/1‑151 (MQ=255)
aTAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTc > 2:462498/1‑151 (MQ=255)
aaataaaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATg > 2:519444/1‑151 (MQ=255)
aaaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTAc > 2:489906/1‑151 (MQ=255)
aaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACg > 2:390916/1‑151 (MQ=255)
gTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACg > 1:559651/1‑119 (MQ=255)
gTTCATTTTATAAACCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACg < 2:559623/119‑1 (MQ=255)
aaaCCAGACATAACGTTCTGCGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTAtt > 1:288546/1‑151 (MQ=255)
|
TAAGACTGATCCCAGCGTTGCGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATT > NC_000913/1787235‑1787512
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
