Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I2 R1	150	68.2	2132745	98.7%	2105019	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,604	G→A	A11V (GCG→GTG)	mepM ←	murein DD‑endopeptidase, space‑maker hydrolase, septation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,604	0	G	A	97.7%	316.9 / NA	87	A11V (GCG→GTG)	mepM	murein DD‑endopeptidase, space‑maker hydrolase, septation protein
Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (28/57); total (28/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCC  >  NC_000913/1941457‑1941750

cgaaTTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCa                                                                                                                                                 <  2:231170/151‑1 (MQ=255)

 gaaTTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACAcc                                                                                                                                                  >  2:385523/1‑149 (MQ=255)

 gaaTTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAg                                                                                                                                                <  1:269130/151‑1 (MQ=255)

 gaaTTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAg                                                                                                                                                <  1:339830/151‑1 (MQ=255)

  aaTTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAgg                                                                                                                                               >  2:796088/1‑151 (MQ=255)

     ttCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGc                                                                                                                                             <  2:609808/150‑1 (MQ=255)

      tCGTTCTGTTGCAGCTCAATGGTTGTGACAATTGGCGTGGCATCACGGTGATAAACAGAAGGCCGCCAGGCGGCGGGGGCCAGAGTAAGAACGGGGAGCGCCCGGAGCGTAGCGCGGTGTGGTCGCGGTGAATTATTAAACGCCGGGGc                                                                                                                                             <  2:589266/149‑1 (MQ=255)

             gTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGc                                                                                                                                     <  1:518930/150‑1 (MQ=255)

             gTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCg                                                                                                                                    <  1:836675/151‑1 (MQ=255)

             gTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCg                                                                                                                                    >  2:874357/1‑151 (MQ=255)

                ccAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCggg                                                                                                                                  <  2:160444/150‑1 (MQ=255)

                ccAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCggg                                                                                                                                  <  1:700723/150‑1 (MQ=255)

                ccAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCggg                                                                                                                                  <  1:1065722/150‑1 (MQ=255)

                ccAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGc                                                                                                                                 <  1:277127/151‑1 (MQ=255)

                    cTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATc                                                                                                                             >  1:152010/1‑151 (MQ=255)

                      cAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCt                                                                                                                            <  2:156687/150‑1 (MQ=255)

                      cAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTg                                                                                                                           <  2:833296/151‑1 (MQ=255)

                          ggTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGc                                                                                                                       <  2:445062/151‑1 (MQ=255)

                               tGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGt                                                                                                                   <  1:1004342/150‑1 (MQ=255)

                                gACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGGCAGCGAGGGCCAGAGGAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTa                                                                                                                  <  2:453824/150‑1 (MQ=255)

                                gACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGGCCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTa                                                                                                                  <  2:253787/150‑1 (MQ=255)

                                 aCAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAAt                                                                                                                <  2:750570/151‑1 (MQ=255)

                                  cAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATc                                                                                                               <  2:99732/151‑1 (MQ=255)

                                  cAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATc                                                                                                               <  1:738739/151‑1 (MQ=255)

                                  cAATTGGCGTGGCATCACGGTGATAAACATAAGGACGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTa                                                                                                                  <  2:738007/148‑1 (MQ=255)

                                   aaTTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATc                                                                                                               >  1:32890/1‑150 (MQ=255)

                                     ttGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACt                                                                                                            >  1:468054/1‑151 (MQ=255)

                                      tGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACtt                                                                                                           >  1:874019/1‑151 (MQ=255)

                                      tGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACt                                                                                                            <  1:422556/150‑1 (MQ=255)

                                          gTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTc                                                                                                       >  1:50530/1‑151 (MQ=255)

                                          gTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTc                                                                                                       <  1:792653/151‑1 (MQ=255)

                                            ggCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCAt                                                                                                     <  2:740475/151‑1 (MQ=255)

                                                tCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAAt                                                                                                 >  2:177042/1‑151 (MQ=255)

                                                       gATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCtt                                                                                           <  2:826777/150‑1 (MQ=255)

                                                          aaaCATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTc                                                                                         >  2:463047/1‑149 (MQ=255)

                                                           aaCATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAgg                                                                                      >  2:420782/1‑151 (MQ=255)

                                                              aTAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGc                                                                                     >  2:799339/1‑149 (MQ=255)

                                                              aTAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGc                                                                                     >  1:280701/1‑149 (MQ=255)

                                                              aTAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAg                                                                                   <  1:908077/151‑1 (MQ=255)

                                                                    ccgccAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCa                                                                             <  1:669439/151‑1 (MQ=255)

                                                                      gccAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATa                                                                           <  2:415993/151‑1 (MQ=255)

                                                                       ccAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATAc                                                                          >  2:120590/1‑151 (MQ=255)

                                                                             aGCTACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAAGCTCCTTTCAGGCAGCTCGCATACTGGtt                                                                     <  2:684692/150‑1 (MQ=255)

                                                                               cGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGc                                                                  <  2:45692/151‑1 (MQ=255)

                                                                                 aCGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCt                                                                 >  1:543481/1‑150 (MQ=255)

                                                                                    gCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAAtt                                                             <  1:326541/151‑1 (MQ=255)

                                                                                        gagTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACt                                                         <  1:372387/151‑1 (MQ=255)

                                                                                         agTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGTTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGAc                                                          <  1:946101/149‑1 (MQ=255)

                                                                                         agTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTc                                                        <  1:482516/151‑1 (MQ=255)

                                                                                            aaGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCGCTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAgg                                                     <  1:150163/151‑1 (MQ=255)

                                                                                            aaGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAg                                                      <  1:856261/150‑1 (MQ=255)

                                                                                               aaCGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAAt                                                  >  2:25958/1‑151 (MQ=255)

                                                                                                 cGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTc                                                <  1:284554/151‑1 (MQ=255)

                                                                                                 cGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTc                                                <  1:117835/151‑1 (MQ=255)

                                                                                                  ggTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCt                                               >  2:515026/1‑151 (MQ=255)

                                                                                                  ggTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCt                                               <  1:1012965/151‑1 (MQ=255)

                                                                                                        cGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAAt                                           >  2:548170/1‑149 (MQ=255)

                                                                                                        cGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAg                                         <  2:875578/151‑1 (MQ=255)

                                                                                                        cGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAg                                         <  1:800283/151‑1 (MQ=255)

                                                                                                             ccAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGt                                    <  2:541972/151‑1 (MQ=255)

                                                                                                                aCATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGtttt                                 <  1:385533/151‑1 (MQ=255)

                                                                                                                aCATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGtttt                                 <  1:92737/151‑1 (MQ=255)

                                                                                                                    aaCGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTAccc                             <  1:356133/151‑1 (MQ=255)

                                                                                                                      cgcgGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAg                           <  1:536334/151‑1 (MQ=255)

                                                                                                                       gcgGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGt                          >  2:949557/1‑151 (MQ=255)

                                                                                                                       gcgGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGt                          >  2:274054/1‑151 (MQ=255)

                                                                                                                        cgGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGtt                         >  1:380198/1‑151 (MQ=255)

                                                                                                                        cgGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGCCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCatt                          <  2:397044/150‑3 (MQ=255)

                                                                                                                          gtgtGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTg                       <  2:566255/151‑1 (MQ=255)

                                                                                                                            gtgGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGat                     >  2:226634/1‑151 (MQ=255)

                                                                                                                            gtgGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGat                     <  2:139287/151‑1 (MQ=255)

                                                                                                                            gtgGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGa                      <  2:732810/150‑1 (MQ=255)

                                                                                                                             tgGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGat                     >  2:841188/1‑150 (MQ=255)

                                                                                                                              ggTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGatat                   >  1:280952/1‑151 (MQ=255)

                                                                                                                              ggTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGatat                   <  2:309423/151‑1 (MQ=255)

                                                                                                                              ggTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGatat                   <  1:440434/151‑1 (MQ=255)

                                                                                                                               gTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGatat                   <  2:715273/150‑1 (MQ=255)

                                                                                                                               gTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATAtt                  <  2:556943/151‑1 (MQ=255)

                                                                                                                                 cgcgGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCg                <  2:150156/151‑1 (MQ=255)

                                                                                                                                  gcgGTGAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGt               <  1:92242/151‑1 (MQ=255)

                                                                                                                                  gcgGTAAATTATTAAACACCAGGGAGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGt               >  1:555808/1‑151 (MQ=255)

                                                                                                                                   cgGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTc              <  1:596969/151‑1 (MQ=255)

                                                                                                                                   cgGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTc              >  1:351668/1‑151 (MQ=255)

                                                                                                                                    ggTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTcc             >  1:800959/1‑151 (MQ=255)

                                                                                                                                        aaTTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAg         <  2:77909/151‑1 (MQ=255)

                                                                                                                                            attaAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGa     <  2:609220/151‑1 (MQ=255)

                                                                                                                                                aaCACCAGGGCGACAGAGCGGGCTATCTATTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATcc  >  2:409524/1‑150 (MQ=255)

CGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCC  >  NC_000913/1941457‑1941750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A