Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I2 R1	150	68.2	2132745	98.7%	2105019	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,951,184	G→A	R118H (CGC→CAC)	yecD →	isochorismatase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,951,184	0	G	A	100.0%	201.7 / NA	52	R118H (CGC→CAC)	yecD	isochorismatase family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/20); total (32/20)

CGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAAC  >  NC_000913/1951037‑1951329

cGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGccaccg                                                                                                                                                >  2:571239/1‑151 (MQ=255)

  gTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAg                                                                                                                                              <  1:595201/151‑1 (MQ=255)

    cGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGt                                                                                                                                            >  1:271362/1‑151 (MQ=255)

    cGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGt                                                                                                                                            <  2:573226/151‑1 (MQ=255)

    cGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGt                                                                                                                                            <  1:909331/151‑1 (MQ=255)

         cgcCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGa                                                                                                                                       <  2:261515/151‑1 (MQ=255)

           cccTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATa                                                                                                                                     <  1:559027/151‑1 (MQ=255)

                  cGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAgt                                                                                                                              <  1:509109/151‑1 (MQ=255)

                   ggCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAgtg                                                                                                                             >  1:1064968/1‑151 (MQ=255)

                    gCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAgtg                                                                                                                             >  1:1038088/1‑150 (MQ=255)

                      tAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGtt                                                                                                                           >  2:579977/1‑150 (MQ=255)

                            gtTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTggg                                                                                                                    <  1:350150/151‑1 (MQ=255)

                             ttGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTggg                                                                                                                    >  2:966191/1‑150 (MQ=255)

                             ttGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTggg                                                                                                                    >  1:997129/1‑150 (MQ=255)

                               gCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGAtc                                                                                                                 <  2:1049720/151‑1 (MQ=255)

                               gCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGAtc                                                                                                                 <  2:883250/151‑1 (MQ=255)

                               gCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGAt                                                                                                                  <  1:825492/150‑1 (MQ=255)

                                     aaaTTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGAcc                                                                                                           >  2:65789/1‑151 (MQ=255)

                                      aaTTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGAcc                                                                                                           >  2:1044666/1‑150 (MQ=255)

                                      aaTTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCa                                                                                                          <  2:654507/151‑1 (MQ=255)

                                         tggtggCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAat                                                                                                        >  1:740797/1‑150 (MQ=255)

                                            tggCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCg                                                                                                    >  2:687375/1‑151 (MQ=255)

                                              gCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGgt                                                                                                  >  1:796025/1‑151 (MQ=255)

                                                   aTCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTg                                                                                              >  2:904815/1‑150 (MQ=255)

                                                         ctgcATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCAc                                                                                       >  1:492126/1‑151 (MQ=255)

                                                               tAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCAccgcccg                                                                                 >  1:924986/1‑151 (MQ=255)

                                                               tAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCAccgcccg                                                                                 >  1:598850/1‑151 (MQ=255)

                                                                  gTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCaa                                                                              <  2:979014/151‑1 (MQ=255)

                                                                  gTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCaa                                                                              <  2:861554/151‑1 (MQ=255)

                                                                   tGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAAt                                                                             >  2:428857/1‑151 (MQ=255)

                                                                       accgaccgCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCt                                                                         >  1:184402/1‑151 (MQ=255)

                                                                       aCCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCt                                                                         >  2:254536/1‑151 (MQ=255)

                                                                              gCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGCATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACt                                                                  <  1:737477/151‑1 (MQ=255)

                                                                               cGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTCGAATCCACCGCCCGCAATGCCTGGGAACtt                                                                 >  1:25575/1‑151 (MQ=255)

                                                                                gATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTg                                                                >  2:810920/1‑151 (MQ=255)

                                                                                     cGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCttt                                                           <  2:356690/151‑1 (MQ=255)

                                                                                          tcatcaAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCt                                                      >  2:490771/1‑151 (MQ=255)

                                                                                                 aCGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGTCCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGAtt                                               >  1:697219/1‑151 (MQ=255)

                                                                                                 aCGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGAtt                                               >  1:797664/1‑151 (MQ=255)

                                                                                                     cAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCg                                           >  2:17772/1‑151 (MQ=255)

                                                                                                      aaTGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGa                                          <  2:174357/151‑1 (MQ=255)

                                                                                                       aTGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGaa                                         <  2:628649/151‑1 (MQ=255)

                                                                                                          gggTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGCGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGAc                                      >  1:656884/1‑151 (MQ=255)

                                                                                                              gCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCt                                  >  1:364596/1‑151 (MQ=255)

                                                                                                                 ttttACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGt                                >  2:659293/1‑150 (MQ=255)

                                                                                                                  tttACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGt                                >  2:694633/1‑149 (MQ=255)

                                                                                                                       ggtacggATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTgccg                         <  2:187084/151‑1 (MQ=255)

                                                                                                                        gtacggATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTgccgc                        <  1:449721/151‑1 (MQ=255)

                                                                                                                          acggATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTgccgcc                       >  2:1042954/1‑150 (MQ=255)

                                                                                                                             gATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAgcg                   >  2:875783/1‑151 (MQ=255)

                                                                                                                                 tGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCg                >  1:500614/1‑150 (MQ=255)

                                                                                                                                  ggAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCg                >  2:681892/1‑149 (MQ=255)

                                                                                                                                             tACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATa    <  2:277919/150‑1 (MQ=255)

                                                                                                                                               cgccacCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAAc  >  1:707464/1‑150 (MQ=255)

CGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAAC  >  NC_000913/1951037‑1951329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A