Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,951,184	G→A	R118H (CGC→CAC)	yecD →	isochorismatase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,951,184	0	G	A	100.0%	185.6 / NA	46	R118H (CGC→CAC)	yecD	isochorismatase family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (30/16); total (30/16)

CGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACA  >  NC_000913/1951037‑1951324

cGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGccaccg                                                                                                                                           <  2:606659/151‑1 (MQ=255)

 ggTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCACACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGccaccg                                                                                                                                           >  2:260789/1‑150 (MQ=255)

 ggTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGa                                                                                                                                          <  1:547662/151‑1 (MQ=255)

          gcCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGAt                                                                                                                                 >  2:93209/1‑151 (MQ=255)

          gcCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGAt                                                                                                                                 >  1:160787/1‑151 (MQ=255)

           cccTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGAt                                                                                                                                 >  1:204932/1‑150 (MQ=255)

               cACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAAt                                                                                                                            <  1:2103/151‑1 (MQ=255)

                 ccGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATa                                                                                                                           <  1:431518/150‑1 (MQ=255)

                 ccGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAg                                                                                                                          <  1:470267/151‑1 (MQ=255)

                  cGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAgt                                                                                                                         >  1:163754/1‑151 (MQ=255)

                   ggCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAgtg                                                                                                                        >  1:319576/1‑151 (MQ=255)

                    gCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAgtgt                                                                                                                       >  2:523526/1‑151 (MQ=255)

                     cTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGtt                                                                                                                      >  2:395194/1‑151 (MQ=255)

                       aaaGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTa                                                                                                                     <  1:120530/150‑1 (MQ=255)

                        aaGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTAtg                                                                                                                   >  1:287828/1‑151 (MQ=255)

                         aGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTAtgt                                                                                                                  >  1:536795/1‑151 (MQ=255)

                           tgtTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTgg                                                                                                                >  1:526018/1‑151 (MQ=255)

                           tgtTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGGGTTATGTgg                                                                                                                >  2:105475/1‑151 (MQ=255)

                             ttGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTggg                                                                                                               >  1:41712/1‑150 (MQ=255)

                                cccGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGAtct                                                                                                           >  2:302401/1‑151 (MQ=255)

                                cccGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGAtct                                                                                                           <  2:593200/151‑1 (MQ=255)

                                cccGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGAtc                                                                                                            >  2:446604/1‑150 (MQ=255)

                                       aTTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCa                                                                                                     >  2:269306/1‑150 (MQ=255)

                                                aaCATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGgtgt                                                                                           >  2:590052/1‑151 (MQ=255)

                                                     ccTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGaa                                                                                       >  1:732896/1‑150 (MQ=255)

                                                     ccTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAAt                                                                                      >  2:751032/1‑151 (MQ=255)

                                                       tgctgcATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATcc                                                                                    >  1:200337/1‑151 (MQ=255)

                                                           gctttAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCAccg                                                                                >  2:514541/4‑151 (MQ=255)

                                                           gcATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCAc                                                                                  <  2:600983/149‑1 (MQ=255)

                                                             aTTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCAccgcc                                                                              >  2:615693/1‑151 (MQ=255)

                                                                 ggTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCa                                                                          <  1:293478/151‑1 (MQ=255)

                                                                 ggTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCa                                                                          <  1:495953/151‑1 (MQ=255)

                                                                        ccGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTg                                                                   <  2:4431/151‑1 (MQ=255)

                                                                         cGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTgg                                                                  >  1:106969/1‑151 (MQ=255)

                                                                         cGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTgg                                                                  >  2:115296/1‑151 (MQ=255)

                                                                           aCAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGa                                                                <  2:536768/151‑1 (MQ=255)

                                                                                   atCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCt                                                        <  2:664335/151‑1 (MQ=255)

                                                                                    tCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCtt                                                       >  1:516910/1‑151 (MQ=255)

                                                                                                  cGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTg                                         >  1:427367/1‑151 (MQ=255)

                                                                                                           ggTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGAc                                 >  2:760540/1‑150 (MQ=255)

                                                                                                              gCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCt                             >  1:284738/1‑151 (MQ=255)

                                                                                                                gTTTTACGGTACGGATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGt                           >  1:619371/1‑151 (MQ=255)

                                                                                                                    tacggtacggATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTg                       >  2:267479/1‑151 (MQ=255)

                                                                                                                          acggATCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCa                 <  1:546681/151‑1 (MQ=255)

                                                                                                                               tCTGGAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGcc            >  2:542256/1‑151 (MQ=255)

                                                                                                                                  ggAGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAgcgc             <  1:745485/147‑1 (MQ=255)

                                                                                                                                    aGTTGCAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGagca       <  1:173504/151‑1 (MQ=255)

                                                                                                                                         cAATTACGCCACCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGcaca  <  1:756619/151‑1 (MQ=255)

CGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAACGTCAATGGGGTGCGTTTTACGGTACGGATCTGGAGTTGCAATTACGCCGCCGAGGTATCGATACAATAGTGTTATGTGGGATCTCGACCAATATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACA  >  NC_000913/1951037‑1951324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A