Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I2 R1 150 68.2 2132745 98.7% 2105019 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,232,230 T→C C129C (TGT→TGC)  cdd → cytidine/deoxycytidine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,232,2300TC98.6% 261.0 / ‑5.9 71C129C (TGT→TGC) cddcytidine/deoxycytidine deaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (38/32);  minor base G (1/0);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGAC  >  NC_000913/2232082‑2232377
                                                                                                                                                    |                                                                                                                                                   
tgAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCgg                                                                                                                                                   >  2:933393/1‑151 (MQ=255)
 gAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCgg                                                                                                                                                   <  1:284471/150‑1 (MQ=255)
    cGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCAc                                                                                                                                               >  1:775121/1‑151 (MQ=255)
    cGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCAc                                                                                                                                               >  2:836897/1‑151 (MQ=255)
     ggAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACt                                                                                                                                              >  1:214600/1‑151 (MQ=255)
     ggAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACt                                                                                                                                              <  2:801753/151‑1 (MQ=255)
          cTGGTATTTCGGTGCCAATATGGAATTTATTGTGGTGACAATGCAGCAAACCGTTCGTGCCGAACAAAGCGCGATCAGCTTCGCCTGGTTGAGTTGTGAAAAAGCGCTTGCAGCCATCGCCGTTAACTACACGCCTTGCGGTCACTg                                                                                                                                             <  1:938945/147‑1 (MQ=255)
          cTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGt                                                                                                                                         >  2:693702/1‑151 (MQ=255)
               aTTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGtt                                                                                                                                    <  2:858499/151‑1 (MQ=255)
               aTTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGtt                                                                                                                                    <  2:730143/151‑1 (MQ=255)
                tttCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGttt                                                                                                                                   >  1:275592/1‑151 (MQ=255)
                      tGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTatgaat                                                                                                                             >  1:745242/1‑151 (MQ=255)
                      tGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTatgaat                                                                                                                             >  1:929687/1‑151 (MQ=255)
                       gCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTatgaat                                                                                                                             <  1:335691/150‑1 (MQ=255)
                        ccAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTatgaatga                                                                                                                           <  1:1023440/151‑1 (MQ=255)
                          aaTATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAAtgaac                                                                                                                         >  2:224120/1‑151 (MQ=255)
                           atatGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAAtgaact                                                                                                                        <  1:793432/151‑1 (MQ=255)
                            tatGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAAtgaactg                                                                                                                       >  1:292597/1‑151 (MQ=255)
                                      aTTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGt                                                                                                              <  1:131287/150‑1 (MQ=255)
                                      aTTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTc                                                                                                             >  2:616152/1‑151 (MQ=255)
                                        tGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCt                                                                                                            <  1:763250/150‑1 (MQ=255)
                                           tGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGAt                                                                                                        >  1:318131/1‑151 (MQ=255)
                                            gCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATc                                                                                                       <  1:585281/151‑1 (MQ=255)
                                            gCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATc                                                                                                       <  1:704738/151‑1 (MQ=255)
                                                cAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCg                                                                                                   >  2:790595/1‑151 (MQ=255)
                                                cAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCg                                                                                                   <  1:100306/151‑1 (MQ=255)
                                                 aaTGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAGGCGCTTGCAGCCATCACGGTAAACTACACCCTTTGGGGCCACTGCCGTAATTTTATGAATAAACGGAACacggttcgggaccgg                                                                                                     >  2:421356/1‑134 (MQ=255)
                                                  aTGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGt                                                                                                  >  1:272886/1‑150 (MQ=255)
                                                  aTGCAGCAAACCGTTCATGCCGAAAAAAGCGCGATCAGCCACGCCTGGATGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGATAACGACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACCGCGGTCTGGATCTGCGt                                                                                                  >  2:772143/1‑150 (MQ=255)
                                                     cagcaAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTc                                                                                              >  1:1022007/1‑151 (MQ=255)
                                                       gcaAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCAt                                                                                            <  1:85810/151‑1 (MQ=255)
                                                         aaaCCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCt                                                                                          >  1:189752/1‑151 (MQ=255)
                                                           aCCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGc                                                                                        >  1:433312/1‑151 (MQ=255)
                                                              gTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCgg                                                                                     <  1:400453/151‑1 (MQ=255)
                                                              gTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCgg                                                                                     >  2:206787/1‑151 (MQ=255)
                                                                  aTGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGcc                                                                                  <  2:652256/150‑1 (MQ=255)
                                                                     ccGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCcgcg                                                                               >  2:472889/1‑150 (MQ=255)
                                                                                gcgATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACgcgc                                                                   >  2:880458/1‑151 (MQ=255)
                                                                                  gATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTg                                                                 <  2:227990/151‑1 (MQ=255)
                                                                                    tCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGc                                                                <  2:996573/150‑1 (MQ=255)
                                                                                      aGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTg                                                             >  2:24150/1‑151 (MQ=255)
                                                                                         cACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACt                                                          >  1:714606/1‑151 (MQ=255)
                                                                                          aCGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTa                                                         <  2:1049539/151‑1 (MQ=255)
                                                                                              cTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTg                                                     >  2:179150/1‑151 (MQ=255)
                                                                                                 gTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGcc                                                   >  2:88042/1‑150 (MQ=255)
                                                                                                 gTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCa                                                  <  2:214592/151‑1 (MQ=255)
                                                                                                        ggtgAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCt                                           <  1:281308/151‑1 (MQ=255)
                                                                                                            aaaaaGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTgg                                       >  1:284257/1‑151 (MQ=255)
                                                                                                              aaaGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGc                                     >  2:857305/1‑151 (MQ=255)
                                                                                                               aaGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGcc                                    <  2:972449/151‑1 (MQ=255)
                                                                                                               aaGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGcc                                    >  1:781682/1‑151 (MQ=255)
                                                                                                                 gcgcTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCg                                   <  1:1049287/150‑1 (MQ=255)
                                                                                                                  cgcTTGCAGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGaa                                 <  1:87920/151‑1 (MQ=255)
                                                                                                                         aGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTg                          <  2:944730/151‑1 (MQ=255)
                                                                                                                         aGCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTg                          >  2:866053/1‑151 (MQ=255)
                                                                                                                          gCCATCACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTgg                         <  2:976610/151‑1 (MQ=255)
                                                                                                                               cACCGTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGAtt                    <  1:357089/151‑1 (MQ=255)
                                                                                                                                   gTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTaaaa                <  2:218533/151‑1 (MQ=255)
                                                                                                                                   gTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTaaa                 >  1:647551/1‑150 (MQ=255)
                                                                                                                                   gTTAACTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTaaa                 >  2:402955/1‑150 (MQ=255)
                                                                                                                                     tAACTACACGCCTTGCGGTCACTGCCGTCAGTCTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAAc               <  1:505133/150‑1 (MQ=255)
                                                                                                                                     tAACTACACGCATTGCGGTCACTGCCGTCAGTGTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAAc               >  2:382275/1‑150 (MQ=255)
                                                                                                                                       aCTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACgct            >  1:424265/1‑151 (MQ=255)
                                                                                                                                       aCTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACgct            >  2:1052718/1‑151 (MQ=255)
                                                                                                                                       aCTACACGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACg              <  2:586683/149‑1 (MQ=255)
                                                                                                                                          acacGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACgctg           <  2:620604/149‑1 (MQ=255)
                                                                                                                                           cacGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGctgctg        >  1:920200/1‑151 (MQ=255)
                                                                                                                                           cacGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGctgctg        <  1:716809/151‑1 (MQ=255)
                                                                                                                                            acGCCTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGctgctg        >  1:15178/1‑150 (MQ=255)
                                                                                                                                               ccTTGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATgg    >  1:578352/1‑151 (MQ=255)
                                                                                                                                                 ttGCGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGAc  >  2:946825/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGAC  >  NC_000913/2232082‑2232377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A