Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I1 R1 121 119.5 3777739 97.6% 3687073 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,655,247 Δ1 bp intergenic (‑106/+100) rspA ← / ← ynfA bifunctional D‑altronate/D‑mannonate dehydratase/UPF0060 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,655,2460G.97.4% 519.0 / 7.0 113intergenic (‑105/+101)rspA/ynfAbifunctional D‑altronate/D‑mannonate dehydratase/UPF0060 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (55/55);  minor base A (2/1);  total (57/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 117 total aligned reads displayed.

ACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCG  >  NC_000913/1655097‑1655393
                                                                                                                                                     |                                                                                                                                                   
aCGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAgc                                                                                                                                                    >  3:160547/1‑150 (MQ=255)
  gCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAgcgt                                                                                                                                                  >  2:446999/1‑148 (MQ=255)
     ccGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGtat                                                                                                                                               >  3:269701/1‑150 (MQ=255)
        gACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATAttt                                                                                                                                           >  3:164227/1‑151 (MQ=255)
        gACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATAttt                                                                                                                                           >  1:67372/1‑151 (MQ=255)
           aGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GAGTATATTTgcg                                                                                                                                        <  2:875127/151‑1 (MQ=255)
            ggTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTgcgc                                                                                                                                       <  3:448698/151‑1 (MQ=255)
             gTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCt                                                                                                                                      <  2:6514/151‑1 (MQ=255)
                aCAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCtgtg                                                                                                                                   >  1:338112/1‑151 (MQ=255)
                aCAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCtgtg                                                                                                                                   >  2:859633/1‑151 (MQ=255)
                   aaaaCTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAAt                                                                                                                                >  3:1014354/1‑151 (MQ=255)
                      aCTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGt                                                                                                                             <  2:10830/151‑1 (MQ=255)
                         tCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGtt                                                                                                                            <  1:59672/149‑1 (MQ=255)
                          cAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGa                                                                                                                          <  3:552517/150‑1 (MQ=255)
                           aGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGAc                                                                                                                         >  4:164456/1‑150 (MQ=255)
                                ttACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACaaa                                                                                                                      >  1:358430/1‑148 (MQ=255)
                                  aCGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATg                                                                                                                 >  3:829403/1‑151 (MQ=255)
                                      tCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAAt                                                                                                             >  2:798273/1‑151 (MQ=255)
                                         tCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAAt                                                                                                             >  3:1177239/1‑148 (MQ=255)
                                         tCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAAt                                                                                                             >  2:649634/1‑148 (MQ=255)
                                         tCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATa                                                                                                            <  4:378115/149‑1 (MQ=255)
                                           aTGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAAt                                                                                                        <  2:174209/151‑1 (MQ=255)
                                             gTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATa                                                                                                       <  1:110418/150‑1 (MQ=255)
                                                cGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCtt                                                                                                   <  4:151504/151‑1 (MQ=255)
                                                cGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCtt                                                                                                   >  2:59026/1‑151 (MQ=255)
                                                 gATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCttt                                                                                                  <  2:760733/151‑1 (MQ=255)
                                                  aTTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTGCTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCtttt                                                                                                 <  2:1089873/151‑1 (MQ=255)
                                                  aTTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCtttt                                                                                                 <  2:124760/151‑1 (MQ=255)
                                                  aTTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCtttt                                                                                                 <  3:902329/151‑1 (MQ=255)
                                                  aTTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCtttt                                                                                                 <  3:504497/151‑1 (MQ=255)
                                                   ttCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTg                                                                                                >  1:51924/1‑151 (MQ=255)
                                                      cTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTc                                                                                              >  3:820733/1‑150 (MQ=255)
                                                        tGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCa                                                                                             <  2:719222/149‑1 (MQ=255)
                                                        tGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAg                                                                                            <  2:186682/150‑1 (MQ=255)
                                                         gCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGc                                                                                           <  1:271700/150‑1 (MQ=255)
                                                         gCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGc                                                                                           <  4:342606/150‑1 (MQ=255)
                                                          cATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTg                                                                                         <  3:1226592/151‑1 (MQ=255)
                                                          cATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTg                                                                                         <  4:358427/151‑1 (MQ=255)
                                                              gCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGacac                                                                                     >  2:69142/1‑151 (MQ=255)
                                                                ttGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACtt                                                                                   >  2:1137097/1‑151 (MQ=255)
                                                                  gTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTc                                                                                  <  3:294992/150‑1 (MQ=255)
                                                                  gTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTc                                                                                  <  1:178376/150‑1 (MQ=255)
                                                                  gTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTCTGTCAGCTGACACTTc                                                                                  <  2:1177183/150‑1 (MQ=255)
                                                                   tCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCtt                                                                                                   >  3:94531/1‑132 (MQ=255)
                                                                    cGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCtt                                                                                                   <  2:94517/131‑1 (MQ=255)
                                                                    cGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCctc                                                                               >  2:466590/1‑151 (MQ=255)
                                                                    cGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCct                                                                                <  3:71787/150‑1 (MQ=255)
                                                                       gATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTTCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCtctt                                                                             <  3:130020/150‑1 (MQ=255)
                                                                       gATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCtctta                                                                            <  3:311593/151‑1 (MQ=255)
                                                                       gATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCtctt                                                                             <  2:599677/150‑1 (MQ=255)
                                                                       gATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCtctt                                                                             <  2:934272/150‑1 (MQ=255)
                                                                        aTGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCtcttat                                                                           >  2:170931/1‑151 (MQ=255)
                                                                          gCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCtcttatc                                                                          <  3:44251/150‑1 (MQ=255)
                                                                              gAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTcttatcttat                                                                      <  2:1014311/150‑1 (MQ=255)
                                                                              gAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTAtt                                                                     <  3:332927/151‑1 (MQ=255)
                                                                              gAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTAtt                                                                     >  3:642419/1‑151 (MQ=255)
                                                                                  tCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGAt                                                                  >  4:39279/1‑150 (MQ=255)
                                                                                  tCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGAt                                                                  >  1:15452/1‑150 (MQ=255)
                                                                                     aCGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATaaaa                                                              <  3:177064/151‑1 (MQ=255)
                                                                                     aCGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATaaa                                                               <  2:610481/150‑1 (MQ=255)
                                                                                      cGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAAt                                                             <  4:53610/151‑1 (MQ=255)
                                                                                         aaCTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATgg                                                           >  3:623024/1‑150 (MQ=255)
                                                                                          aCTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGAt                                                         <  4:412055/151‑1 (MQ=255)
                                                                                            tGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCATTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGAttt                                                       >  3:416047/1‑151 (MQ=255)
                                                                                             gAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGAttt                                                       >  2:1105286/1‑150 (MQ=255)
                                                                                              aGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTAt                                                     >  3:126524/1‑151 (MQ=255)
                                                                                               gCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATg                                                    <  2:1086622/151‑1 (MQ=255)
                                                                                                ctactaCCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATg                                                    <  1:238443/150‑1 (MQ=255)
                                                                                                ctactaCCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGt                                                   >  1:115828/1‑151 (MQ=255)
                                                                                                  actacCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGt                                                   <  3:340074/149‑1 (MQ=255)
                                                                                                     acCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCt                                               >  2:752286/1‑150 (MQ=255)
                                                                                                      ccATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTAc                                             >  2:1114453/1‑151 (MQ=255)
                                                                                                          aCAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                         <  2:338223/151‑1 (MQ=255)
                                                                                                          aCAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACCTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                         >  2:386977/1‑151 (MQ=255)
                                                                                                           cAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg                                        >  1:311009/1‑151 (MQ=255)
                                                                                                                 taAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGccccc                                   >  2:801526/1‑150 (MQ=255)
                                                                                                                  aaaGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGccccc                                   >  1:318993/1‑149 (MQ=255)
                                                                                                                  aaaGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAg                                 <  3:859671/151‑1 (MQ=255)
                                                                                                                    aGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGcc                               >  2:475378/1‑151 (MQ=255)
                                                                                                                    aGATCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGcc                               <  1:371712/151‑1 (MQ=255)
                                                                                                                      aTCGAAAAAAGCCCGAGTGATCACAACAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCa                                  >  4:183349/1‑146 (MQ=255)
                                                                                                                       tCGAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGc                            >  2:570953/1‑151 (MQ=255)
                                                                                                                         gAAAAAAGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGca                           >  4:411004/1‑150 (MQ=255)
                                                                                                                          aaaaaaGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGcaac                         >  2:442228/1‑151 (MQ=255)
                                                                                                                           aaaaaGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTGTCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGcaaca                        <  3:1249966/151‑1 (MQ=255)
                                                                                                                            aaaaGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGcaaca                        >  3:149475/1‑150 (MQ=255)
                                                                                                                            aaaaGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGcaaca                        >  1:106334/1‑150 (MQ=255)
                                                                                                                            aaaaGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGcaaca                        >  3:935088/1‑150 (MQ=255)
                                                                                                                             aaaGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAAt                      >  2:1227480/1‑151 (MQ=255)
                                                                                                                              aaGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATg                     >  3:5972/1‑151 (MQ=255)
                                                                                                                              aaGCCGGAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATg                     >  2:51153/1‑151 (MQ=255)
                                                                                                                                   ggAGTGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCa                 >  3:903685/1‑150 (MQ=255)
                                                                                                                                       tGATCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACAt             >  3:1227376/1‑150 (MQ=255)
                                                                                                                                         aTCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGcc          <  2:828487/151‑1 (MQ=255)
                                                                                                                                          tCACAAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCg         >  3:28707/1‑151 (MQ=255)
                                                                                                                                           cacaAAAAAA‑GCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCg         <  2:1179651/150‑1 (MQ=255)
                                                                                                                                             acaaaaaaAGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACCTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCa       <  3:387013/149‑1 (MQ=255)
                                                                                                                                              caaaaaaAGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCa       >  4:209721/2‑150 (MQ=255)
                                                                                                                                                aaaaaaGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCaa      >  3:99148/1‑149 (MQ=255)
                                                                                                                                                  aaaaGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCAAAgcg  <  3:620206/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCG  >  NC_000913/1655097‑1655393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A