Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,664,969	T→C	L155P (CTT→CCT)	dmsD →	twin‑argninine leader‑binding protein for DmsA and TorA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,664,969	0	T	C	100.0%	173.6 / NA	46	L155P (CTT→CCT)	dmsD	twin‑argninine leader‑binding protein for DmsA and TorA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/21); total (25/21)

CGTCAGTGGATGCGCGAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCTTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCG  >  NC_000913/1664824‑1665116

cGTCAGTGGATGCGCGAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCctttt                                                                                                                                                 <  2:219962/150‑1 (MQ=255)

  tCAGTGGATGCGCGAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCg                                                                                                                                              >  1:504611/1‑151 (MQ=255)

       ggATGCGCGAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGt                                                                                                                                          <  2:504916/150‑1 (MQ=255)

       ggATGCGCGAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTc                                                                                                                                         >  1:444204/1‑151 (MQ=255)

            cgcgAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAacac                                                                                                                                    >  2:60197/1‑151 (MQ=255)

             gcgAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACg                                                                                                                                   <  1:475968/151‑1 (MQ=255)

                 gaAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGtttt                                                                                                                               >  1:682730/1‑151 (MQ=255)

                    aGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCtt                                                                                                                            <  2:546444/151‑1 (MQ=255)

                          tCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGt                                                                                                                       <  2:512305/150‑1 (MQ=255)

                          tCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGt                                                                                                                       <  1:224079/150‑1 (MQ=255)

                            aGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGtttt                                                                                                                    <  1:359373/151‑1 (MQ=255)

                                  aaaTGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGa                                                                                                              <  2:627426/151‑1 (MQ=255)

                                      gAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGaaaaa                                                                                                          <  1:654230/151‑1 (MQ=255)

                                      gAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGaaaa                                                                                                           <  1:541156/150‑1 (MQ=255)

                                        aGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGc                                                                                                        <  1:8976/151‑1 (MQ=255)

                                            aaaCGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGa                                                                                                     >  2:430510/1‑150 (MQ=255)

                                               cGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAacac                                                                                                 >  2:327010/1‑151 (MQ=255)

                                                  aCCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCt                                                                                              <  2:478003/151‑1 (MQ=255)

                                                     ggAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTc                                                                                           >  2:649420/1‑151 (MQ=255)

                                                       aaGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCt                                                                                          >  2:432695/1‑150 (MQ=255)

                                                         gATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTAcc                                                                                       >  1:616094/1‑151 (MQ=255)

                                                          aTCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAAAACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCg                                                                                      >  1:161674/1‑151 (MQ=255)

                                                           tCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGt                                                                                     >  2:607951/1‑151 (MQ=255)

                                                            cATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTg                                                                                    <  1:4000/151‑1 (MQ=255)

                                                            cATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTg                                                                                    >  1:97423/1‑151 (MQ=255)

                                                              ttttGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGc                                                                                   >  2:337935/1‑150 (MQ=255)

                                                                       aCTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGa                                                                          >  1:284367/1‑150 (MQ=255)

                                                                           ctgTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTgg                                                                     >  1:652095/1‑151 (MQ=255)

                                                                                gATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGc                                                                <  1:440515/151‑1 (MQ=255)

                                                                                   ggCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCtt                                                              >  2:589084/1‑150 (MQ=255)

                                                                                     cTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTACCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAAc                                                           >  2:479955/1‑151 (MQ=255)

                                                                                      tGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACg                                                          >  2:636387/1‑151 (MQ=255)

                                                                                           ggTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGgc                                                     <  1:148575/151‑1 (MQ=255)

                                                                                                         ggTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATcac                                       >  1:28222/1‑151 (MQ=255)

                                                                                                         ggTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATca                                        <  2:202151/150‑1 (MQ=255)

                                                                                                           tCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATcaca                                      >  2:407014/1‑150 (MQ=255)

                                                                                                            cGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACaa                                     <  2:76333/150‑1 (MQ=255)

                                                                                                              ccAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTg                                  >  2:150678/1‑151 (MQ=255)

                                                                                                              ccAGACAGAATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTg                                  >  2:125340/1‑151 (MQ=255)

                                                                                                                  acagaATGCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTa                               >  2:247315/1‑150 (MQ=255)

                                                                                                                         gCGAAGAATTGCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTg                        <  2:304194/150‑1 (MQ=255)

                                                                                                                                   gCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTaa              >  2:620509/1‑150 (MQ=255)

                                                                                                                                   gCTGGCATGGCACCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTaa              <  2:620204/150‑1 (MQ=255)

                                                                                                                                     tggcatggcaCTCTTTTCCGTGGTCAACACGGTTTCTTGATGTTTCTATCGAAAAAGCAGAACACCCTTTTCACCGTGCACTGGGTGAACTGGCGCGCTTAACGTTGGCGCAATAGCAATCACAACTGTTAATTCCTGTCACGGTTAAAcc           >  1:137483/1‑151 (MQ=255)

                                                                                                                                      ggcatggcaCCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAAcc           <  2:678833/150‑1 (MQ=255)

                                                                                                                                      ggcatggcaCCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAAcc           <  2:7744/150‑1 (MQ=255)

                                                                                                                                              aCCCTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCg  <  2:357702/151‑1 (MQ=255)

CGTCAGTGGATGCGCGAGAAAGGCATTCAGTTTGAAATGAAGCAAAACGAACCGGAAGATCATTTTGGTTCACTGCTGTTGATGGCTGCGTGGTTGGCGGAGAATGGTCGCCAGACAGAATGCGAAGAATTGCTGGCATGGCACCTTTTTCCGTGGTCAACACGTTTTCTTGATGTTTTTATCGAAAAAGCAGAACACCCTTTCTACCGTGCACTGGGTGAACTGGCTCGCTTAACGCTGGCGCAATGGCAATCACAACTGTTAATTCCTGTCGCGGTTAAACCGCTGTTTCG  >  NC_000913/1664824‑1665116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A