Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 160 117.2 4331776 85.4% 3699336 149.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,684,983 T→C V242A (GTC→GCC)  tus → inhibitor of replication at Ter, DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,684,9830TC99.1% 375.7 / NA 106V242A (GTC→GCC) tusinhibitor of replication at Ter, DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (46/59);  total (47/59)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

AGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTGCAGCACCGTTATAAACC  >  NC_000913/1684835‑1685130
                                                                                                                                                     |                                                                                                                                                   
aGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCt                                                                                                                                                   >  2:417743/1‑151 (MQ=255)
aGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCt                                                                                                                                                   >  3:209752/1‑151 (MQ=255)
  ccTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTgg                                                                                                                                                 <  4:293609/151‑1 (MQ=255)
    tGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGt                                                                                                                                                >  2:1550807/1‑150 (MQ=255)
     gAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTAc                                                                                                                                              <  3:301588/151‑1 (MQ=255)
        aTCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACaaa                                                                                                                                           <  4:1496314/151‑1 (MQ=255)
            ccacGCAGGGGCGCACCGGGGACGCGCGAGGAGGGGCAAAGAAAA‑CGGGAGCGAGAGGAGCAGGAGAGCGCGGCCCGGCCACAGAACGCGAAGTTAAAAATCAAACGGCCGGGGAAGGGGCAGCCGATTGCCCGCGCCTGGTACAAAGgag                                                                                                                                       <  2:1150213/151‑1 (MQ=255)
             cacGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAgg                                                                                                                                         >  2:191711/1‑148 (MQ=255)
              acGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAgg                                                                                                                                         >  1:427205/1‑147 (MQ=255)
              acGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAgg                                                                                                                                         >  1:57231/1‑147 (MQ=255)
                gCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATc                                                                                                                                    >  4:458562/1‑150 (MQ=255)
                gCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATc                                                                                                                                    >  2:1358279/1‑150 (MQ=255)
                gCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCa                                                                                                                                   <  4:1382917/151‑1 (MQ=255)
                    tgtCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCaaaa                                                                                                                                >  2:1316813/1‑150 (MQ=255)
                       cGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACaa                                                                                                                            <  4:25165/151‑1 (MQ=255)
                       cGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACaa                                                                                                                            <  4:598527/151‑1 (MQ=255)
                          aCCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTc                                                                                                                         <  2:108492/151‑1 (MQ=255)
                          aCCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTc                                                                                                                         >  2:569873/1‑151 (MQ=255)
                           ccGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTcc                                                                                                                        >  4:478353/1‑151 (MQ=255)
                              tGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAac                                                                                                                     >  2:6176/1‑151 (MQ=255)
                               ggACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAac                                                                                                                     <  4:119134/150‑1 (MQ=255)
                                 aCGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACg                                                                                                                  <  4:70279/151‑1 (MQ=255)
                                 aCGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACg                                                                                                                  >  4:788810/1‑151 (MQ=255)
                                  cgcgcgAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGc                                                                                                                 >  2:1460165/1‑151 (MQ=255)
                                      cgAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGc                                                                                                             >  2:1359889/1‑151 (MQ=255)
                                       gaggagTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGcc                                                                                                            <  4:1092569/151‑1 (MQ=255)
                                       gaggagTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGcc                                                                                                            >  2:960539/1‑151 (MQ=255)
                                        aggagTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGccc                                                                                                           >  3:416266/1‑151 (MQ=255)
                                          ggggggCAAAGAAAATCTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGcct                                                                                                           <  1:240771/146‑2 (MQ=255)
                                          gagTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCt                                                                                                          >  4:267195/1‑150 (MQ=255)
                                                 aaaGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACt                                                                                                  <  2:118922/151‑1 (MQ=255)
                                                 aaaGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACt                                                                                                  <  2:1439432/151‑1 (MQ=255)
                                                 aaaGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCGGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTAcaccac                                                                                                   <  3:83496/150‑1 (MQ=255)
                                                  aaGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTg                                                                                                 >  1:34940/1‑151 (MQ=255)
                                                  aaGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTg                                                                                                 >  4:1612690/1‑151 (MQ=255)
                                                     aaaa‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGAtt                                                                                              >  4:674298/1‑151 (MQ=255)
                                                      aaa‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTg                                                                                             <  3:356729/151‑1 (MQ=255)
                                                       aa‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGc                                                                                            >  4:1449412/1‑151 (MQ=255)
                                                             gAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTg                                                                                        >  2:758848/1‑150 (MQ=255)
                                                               gCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGAtg                                                                                     <  4:1136596/151‑2 (MQ=255)
                                                                cGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGAtt                                                                                     >  2:611702/1‑150 (MQ=255)
                                                                 gagaGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTaa                                                                                   <  4:1008628/151‑1 (MQ=255)
                                                                    agTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCg                                                                                >  2:1375171/1‑151 (MQ=255)
                                                                      tagcAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCgg                                                                               <  2:1290361/147‑1 (MQ=255)
                                                                       aTCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCgg                                                                               <  2:992648/149‑1 (MQ=255)
                                                                       aTCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGa                                                                             <  2:823890/151‑1 (MQ=255)
                                                                         cAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGAt                                                                            >  2:1100209/1‑150 (MQ=255)
                                                                         cAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATa                                                                           <  2:1581446/151‑1 (MQ=255)
                                                                             atatCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAAt                                                                         >  3:77573/1‑149 (MQ=255)
                                                                               atCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGgcg                                                                     >  3:206707/1‑151 (MQ=255)
                                                                                  gCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGcgcg                                                                   <  2:1017812/150‑1 (MQ=255)
                                                                                  gCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCgg                                                                  <  2:1534010/151‑1 (MQ=255)
                                                                                   ctgccctgccACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCgg                                                                  <  4:167696/150‑1 (MQ=255)
                                                                                    tgccctgccACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGc                                                                >  3:241128/1‑151 (MQ=255)
                                                                                      ccctgccACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGt                                                              <  2:1567312/151‑1 (MQ=255)
                                                                                        ctgccACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGc                                                            >  2:553865/1‑151 (MQ=255)
                                                                                          gccACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGc                                                            <  4:150536/149‑1 (MQ=255)
                                                                                            cacaGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGa                                                        <  2:90078/151‑1 (MQ=255)
                                                                                             acaGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGAc                                                       <  4:389947/151‑1 (MQ=255)
                                                                                             acaGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGAc                                                       <  4:489273/151‑1 (MQ=255)
                                                                                                 aaCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTg                                                   >  1:147969/1‑151 (MQ=255)
                                                                                                  aCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTgg                                                  >  1:362758/1‑151 (MQ=255)
                                                                                                      gAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAg                                              <  2:1152658/151‑1 (MQ=255)
                                                                                                        aGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAgtt                                            >  2:852445/1‑151 (MQ=255)
                                                                                                         gTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAgttg                                           <  2:95148/151‑1 (MQ=255)
                                                                                                         gTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAgttg                                           <  4:346374/151‑1 (MQ=255)
                                                                                                          ttAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAgttgt                                          >  3:399549/1‑151 (MQ=255)
                                                                                                          ttAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAgttg                                           <  3:171664/150‑1 (MQ=255)
                                                                                                            aaaaaTCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAgttgtt                                         <  4:630142/150‑1 (MQ=255)
                                                                                                             aaaaTCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTaa                                       <  3:256989/151‑1 (MQ=255)
                                                                                                                aTCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAAtt                                    <  4:881420/151‑1 (MQ=255)
                                                                                                                 tCACACGACAGGTGAAGGTGCAGCAGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGAGTGCCGGACGTTGGTGCGTTGTTAAAt                                     >  2:1528429/1‑149 (MQ=255)
                                                                                                                  cAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTAc                                  <  2:1455317/151‑1 (MQ=255)
                                                                                                                  cAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTAc                                  >  4:1034649/1‑151 (MQ=255)
                                                                                                                   aaaCGAACGGTGAAGGCGCTGCCGATTGCCCGCGTCTGGTACAAAGGAGCTCAAAAACAAGTCCAACACGCCAGACCTACACCACTGATTGCACTGATTATTCGGGATAATGGCGCGGCCGTGCCGGACGTTGGTGAGTTGTTAAATTa                                   >  2:704669/1‑149 (MQ=255)
                                                                                                                      cGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGAt                               <  2:673375/150‑1 (MQ=255)
                                                                                                                      cGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATg                              >  2:1534794/1‑151 (MQ=255)
                                                                                                                      cGTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATg                              <  4:1318460/151‑1 (MQ=255)
                                                                                                                       gTCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGc                             >  2:595970/1‑151 (MQ=255)
                                                                                                                        tCCGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGcc                            >  4:160538/1‑151 (MQ=255)
                                                                                                                         ccGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGcc                            <  4:1486625/150‑1 (MQ=255)
                                                                                                                         ccGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGcc                            <  4:1159613/150‑1 (MQ=255)
                                                                                                                         ccGGTGAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCg                           <  2:927026/151‑1 (MQ=255)
                                                                                                                              gAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAAt                      <  2:525611/151‑1 (MQ=255)
                                                                                                                              gAAGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAAt                      <  4:685569/151‑1 (MQ=255)
                                                                                                                                aGGTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAAtgt                    <  4:221273/151‑1 (MQ=255)
                                                                                                                                  gTGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTgc                  <  4:1467675/151‑1 (MQ=255)
                                                                                                                                   tGCAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTgc                  >  4:1454593/1‑150 (MQ=255)
                                                                                                                                     cAGCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTgcagc               <  2:47940/151‑1 (MQ=255)
                                                                                                                                       gCCGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTgcagca              <  1:392963/150‑1 (MQ=255)
                                                                                                                                         cGATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTGCAGCACCg           <  4:831764/151‑1 (MQ=255)
                                                                                                                                          gATTGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTGCAGCACCGt          <  4:313568/151‑1 (MQ=255)
                                                                                                                                            ttGCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGGCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATGACGATGCCGACAATGTGCAGCATCGtt         <  2:626544/150‑1 (MQ=255)
                                                                                                                                              gCCCGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTGCAGCACCGTtat       <  2:1390816/150‑1 (MQ=255)
                                                                                                                                               cccGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTGCAGCACCGTTATaa     <  2:1567746/151‑1 (MQ=255)
                                                                                                                                                ccGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTGCAGCACCGTTATaaa    <  3:433774/151‑1 (MQ=255)
                                                                                                                                                ccGCGCCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTGCAGCACCGTTATaa     >  4:1576003/1‑150 (MQ=255)
                                                                                                                                                  gcgcCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTGCAGCACCGTTATAAAcc  <  2:787771/151‑1 (MQ=255)
                                                                                                                                                  gcgcCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTGCAGCACCGTTATAAAcc  >  2:954144/1‑151 (MQ=255)
                                                                                                                                                  gcgcCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTGCAGCACCGTTATAAAcc  >  2:1431269/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAA‑CTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAATTACGATGCCGACAATGTGCAGCACCGTTATAAACC  >  NC_000913/1684835‑1685130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A