breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 25,559 A→G H118R (CAC→CGC)  lspA → prolipoprotein signal peptidase (signal peptidase II)
RA 34,120 (T)9→8 intergenic (+82/‑180) carB → / → caiF carbamoyl‑phosphate synthase large subunit/cai operon transcriptional activator
RA 54,593 A→G V37A (GTG→GCG)  surA ← peptidyl‑prolyl cis‑trans isomerase (PPIase)
RA 57,698 G→A R112H (CGC→CAC)  djlA → membrane‑anchored DnaK co‑chaperone, DNA‑binding protein
RA 77,913 C→T A98V (GCA→GTA)  setA → broad specificity sugar efflux system
RA 92,324 A→G E304E (GAA→GAG ftsI → transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3
RA 205,820 G→A R232H (CGT→CAT)  dnaE → DNA polymerase III alpha subunit
RA 230,758 C→T E42K (GAG→AAG)  yafC ← LysR family putative transcriptional regulator
RA 257,644 A→G D373G (GAC→GGC)  frsA → fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
MC JC 257,908 Δ776 bp [crl] [crl]
RA 311,012 T→C S109G (AGC→GGC)  ecpR ← putative transcriptional regulator for the ecp operon
RA 322,322 C→T R239C (CGC→TGC)  ykgE → cysteine‑rich LutA family protein; putative electron transport chain YkgEFG component
RA 379,275 A→G R99R (CGT→CGC frmA ← alcohol dehydrogenase class III; glutathione‑dependent formaldehyde dehydrogenase
RA 396,507 A→G intergenic (‑220/‑132) ampH ← / → sbmA D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase/peptide antibiotic transporter
RA 428,067 A→G T141A (ACC→GCC)  secD → SecYEG protein translocase auxillary subunit
RA 455,102 A→G intergenic (+313/‑31) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
RA 456,232 T→C V367A (GTG→GCG)  tig → peptidyl‑prolyl cis/trans isomerase (trigger factor)
RA 489,636 A→G H1034R (CAC→CGC)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated
RA 511,225 A→G L52P (CTG→CCG)  copA ← copper transporter
RA 554,198 A→G R80R (CGT→CGC ppiB ← peptidyl‑prolyl cis‑trans isomerase B (rotamase B)
RA 562,993 C→T P220S (CCT→TCT)  sfmH → FimA homolog, function unknown
RA 592,031 T→C Y383C (TAT→TGT)  nfrB ← bacteriophage N4 receptor, inner membrane subunit
RA 667,766 T→C Y151C (TAC→TGC)  mrdA ← penicillin‑binding protein 2, transpeptidase involved in peptidoglycan synthesis
RA 699,271 T→C intergenic (‑94/+303) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 724,758 T→C E74E (GAA→GAG kdpC ← potassium translocating ATPase, subunit C
RA 756,306 A→G T134A (ACT→GCT)  sdhA → succinate dehydrogenase, flavoprotein subunit
RA 759,716 T→C R337R (CGT→CGC sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
RA 768,193 T→C R72R (CGT→CGC mngB → alpha‑mannosidase
RA 804,844 C→T R448C (CGC→TGC)  ybhJ → aconitase family protein
RA 808,658 C→T A200A (GCG→GCA bioA ← 7,8‑diaminopelargonic acid synthase, PLP‑dependent
RA 886,797 T→C A181A (GCA→GCG ybjJ ← putative drug efflux MFS transporter, inner membrane protein
RA 887,114 T→C T76A (ACA→GCA)  ybjJ ← putative drug efflux MFS transporter, inner membrane protein
JC JC 944,912 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp intergenic (+16/+14) dmsC → / ← ycaC dimethyl sulfoxide reductase, anaerobic, subunit C/putative isochorismatase family hydrolase
RA 976,248 A→G E209E (GAA→GAG mukE → chromosome condensin MukBEF, MukE localization factor
RA 981,315 A→G Y90C (TAT→TGT)  ldtD → murein L,D‑transpeptidase
RA 994,513 A→G V107A (GTG→GCG)  ssuC ← aliphatic sulfonate ABC transporter permease
RA 1,007,093 G→A Q170* (CAA→TAA)  ycbX ← 6‑N‑hydroxylaminopurine detoxification oxidoreductase
RA 1,007,235 C→T W122* (TGG→TGA ycbX ← 6‑N‑hydroxylaminopurine detoxification oxidoreductase
RA 1,061,552 G→A R766Q (CGA→CAA)  torA → trimethylamine N‑oxide (TMAO) reductase I, catalytic subunit
RA 1,071,745 A→G P7P (CCT→CCC rutD ← putative reactive intermediate detoxifying aminoacrylate hydrolase
RA 1,072,993 A→G V340A (GTG→GCG)  rutA ← pyrimidine oxygenase, FMN‑dependent
RA 1,109,596 T→C Y88H (TAC→CAC)  opgG → OPG biosynthetic periplasmic beta‑1,6 branching glycosyltransferase
RA 1,111,899 A→G D346G (GAT→GGT)  opgH → OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist
RA 1,147,646 A→G D9G (GAT→GGT)  plsX → putative phosphate acyltransferase
RA 1,163,318 C→T P20L (CCG→CTG)  thiK → thiamine kinase
RA 1,228,980 C→T R56C (CGC→TGC)  ycgN → UPF0153 family cysteine cluster protein
RA 1,233,845 A→G R291R (CGT→CGC nhaB ← sodium:proton antiporter
RA 1,260,214 (A)6→5 coding (590/1680 nt) dauA ← C4‑dicarboxylic acid transporter
RA 1,306,946 (A)6→7 coding (205/330 nt) yciU ← UPF0263 family protein
RA 1,307,362 C→T W428* (TGG→TGA clsA ← cardiolipin synthase 1
RA 1,325,092 T→C V116A (GTG→GCG)  yciQ → enhancer of membrane protein expression; putative inner membrane protein
RA 1,370,044 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein
RA 1,379,556 T→C L558P (CTG→CCG)  ycjT → putative family 65 glycosyl hydrolase
RA 1,399,186 T→C Q114R (CAG→CGG)  fnr ← oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor
RA 1,416,148 A→G H413H (CAT→CAC recE ← Rac prophage; exonuclease VIII, 5' to 3' specific dsDNA exonuclease
RA 1,419,242 A→G F24L (TTC→CTC)  ydaF ← uncharacterized protein, Rac prophage
RA 1,476,832 T→C Y563Y (TAT→TAC ydbD → PF10971 family putative periplasmic methylglyoxal resistance protein
RA 1,541,618 C→T D325N (GAC→AAC)  narZ ← nitrate reductase 2 (NRZ), alpha subunit
RA 1,582,994 T→C K232E (AAG→GAG)  ydeO ← UV‑inducible global regulator, EvgA‑, GadE‑dependent
RA 1,585,827 A→G G220G (GGT→GGC ydeP ← putative oxidoreductase
RA 1,594,132 (C)8→9 pseudogene (3856/3861 nt) yneO ← pseudogene, AidA homolog
RA 1,618,206 A→G E393E (GAA→GAG ydeA → arabinose efflux transporter, arabinose‑inducible
RA 1,684,983 T→C V242A (GTC→GCC)  tus → inhibitor of replication at Ter, DNA‑binding protein
RA 1,693,262 G→A G334G (GGC→GGT uidB ← glucuronide transporter
RA 1,698,899 T→C G94G (GGA→GGG malI ← transcriptional repressor of Mal regulon
RA 1,703,968 A→G L114P (CTG→CCG)  ydgJ ← putative oxidoreductase
RA 1,797,110 G→A R279W (CGG→TGG)  pheT ← phenylalanine tRNA synthetase, beta subunit
RA 1,816,806 A→G R110R (CGT→CGC chbG ← chito‑oligosaccharide deacetylase
RA 1,867,340 A→T N145Y (AAC→TAC)  yeaG → protein kinase, endogenous substrate unidentified; autokinase
RA 1,920,830 T→C V203A (GTG→GCG)  rsmF → 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
RA 1,925,601 A→G R54R (CGA→CGG yobB → C‑N hydrolase family protein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,003,932 A→G S21G (AGC→GGC)  fliD → flagellar filament capping protein
RA 2,093,269 A→G Q291R (CAG→CGG)  hisC → histidinol‑phosphate aminotransferase
RA 2,150,798 T→C D63G (GAC→GGC)  yegI ← protein kinase‑related putative non‑specific DNA‑binding protein
RA 2,170,145 T→C pseudogene (23/475 nt) gatR ← pseudogene, repressor for gat operon; interrupted by IS3; split galactitol utilization operon repressor, fragment 2; split galactitol utilization operon repressor, interrupted
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,206,043 A→G T117A (ACG→GCG)  yehM → uncharacterized protein
RA 2,213,613 A→T G344G (GGT→GGA yehU ← inner membrane putative sensory kinase in two‑component system with YehT
RA 2,315,572 C→T C695C (TGC→TGT rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
RA 2,315,727 G→A G747D (GGC→GAC)  rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
RA 2,371,563 T→C V219A (GTT→GCT)  arnT → 4‑amino‑4‑deoxy‑L‑arabinose transferase
RA 2,373,305 T→C E78E (GAA→GAG pmrD ← inactive two‑component system connector protein
RA 2,413,228 T→C intergenic (‑96/‑242) yfbV ← / → ackA UPF0208 family inner membrane protein/acetate kinase A and propionate kinase 2
RA 2,461,278 A→G intergenic (‑344/‑28) yfcZ ← / → fadL UPF0381 family protein/long‑chain fatty acid outer membrane transporter
RA 2,479,993 T→C R264R (CGT→CGC dsdA → D‑serine dehydratase
RA 2,484,572 A→G T67A (ACG→GCG)  evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA
RA 2,490,592 A→G S453S (AGT→AGC oxc ← oxalyl CoA decarboxylase, ThDP‑dependent
RA 2,521,359 (T)6→7 intergenic (+31/+234) lysV → / ← xapR tRNA‑Lys/transcriptional activator of xapAB
RA 2,527,479 G→A G178G (GGG→GGA yfeH → putative inorganic ion transporter
RA 2,550,463 T→C D28G (GAC→GGC)  yfeX ← porphyrinogen oxidase, cytoplasmic
RA 2,596,461 T→C T93A (ACC→GCC)  ypfJ ← putative neutral zinc metallopeptidase
RA 2,621,017 A→G intergenic (‑145/‑180) upp ← / → purM uracil phosphoribosyltransferase/phosphoribosylaminoimidazole synthetase
RA 2,700,875 T→C T42A (ACG→GCG)  acpS ← holo‑[acyl‑carrier‑protein] synthase 1
RA 2,711,683 T→C W422R (TGG→CGG)  nadB → quinolinate synthase, L‑aspartate oxidase (B protein) subunit
RA 2,712,443 A→G W108R (TGG→CGG)  trmN ← tRNA1(Val) (adenine(37)‑N6)‑methyltransferase
RA 2,733,986 T→C D63G (GAT→GGT)  clpB ← protein disaggregation chaperone
RA 2,734,168 A→G R2R (CGT→CGC clpB ← protein disaggregation chaperone
RA 2,809,774 (T)7→6 coding (158/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein
RA 2,821,424 G→C G196G (GGC→GGG alaS ← alanyl‑tRNA synthetase
RA 2,838,061 G→A C15C (TGC→TGT hydN ← formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit
RA 2,896,700 G→A P391S (CCG→TCG)  ygcS ← putative MFS sugar transporter; membrane protein
RA 2,905,028 T→C T131A (ACC→GCC)  queE ← 7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA 2,908,834 T→C Q278R (CAG→CGG)  pyrG ← CTP synthetase
RA 2,912,357 T→C H432R (CAC→CGC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,915,854 T→C R266R (CGT→CGC barA → hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
RA 2,947,095 G→A R28R (CGC→CGT mltA ← membrane‑bound lytic murein transglycosylase A
RA 3,044,702 T→C S347S (TCT→TCC bglA → 6‑phospho‑beta‑glucosidase A
RA 3,085,843 C→T Q23Q (CAG→CAA speA ← biosynthetic arginine decarboxylase, PLP‑binding
RA 3,089,713 A→G intergenic (+35/‑42) galP → / → yggI D‑galactose transporter/Zn‑dependent metalloprotease‑related protein
RA 3,094,280 C→T V267V (GTG→GTA yggR ← putative PilT family AAA+ ATPase
RA 3,124,632 A→G S276S (AGT→AGC glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
RA 3,179,085 T→C V324A (GTG→GCG)  tolC → transport channel
RA 3,197,294 T→C E783G (GAA→GGA)  glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
RA 3,206,722 T→C F87S (TTC→TCC)  ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,214,171 G→A A375A (GCG→GCA rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 3,243,419 T→C E441E (GAA→GAG uxaC ← uronate isomerase
RA 3,297,157 T→C T328A (ACA→GCA)  yraQ ← putative inner membrane permease
RA 3,297,214 T→C S309G (AGT→GGT)  yraQ ← putative inner membrane permease
RA 3,332,634 T→C E34E (GAA→GAG yhbE ← EamA family inner membrane putative transporter
RA 3,340,559 C→A T95T (ACC→ACA yrbG → putative calcium/sodium:proton antiporter
RA 3,352,312 G→A R238R (CGC→CGT arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
RA 3,377,215 A→G L69P (CTG→CCG)  sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
RA 3,485,341 A→G E284E (GAA→GAG prkB → putative phosphoribulokinase
RA 3,523,544 T→C V225A (GTG→GCG)  mrcA → penicillin‑binding protein 1a, murein transglycosylase and transpeptidase
RA 3,545,025 T→C H48H (CAT→CAC gntX → DNA catabolic protein
RA 3,550,505 T→C T657A (ACG→GCG)  malP ← maltodextrin phosphorylase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,616,605 G→A A142T (GCC→ACC)  nikC → nickel ABC transporter permease
RA 3,627,512 G→A A676V (GCC→GTC)  rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
RA 3,636,906 C→T A169T (GCG→ACG)  yhiN ← putative oxidoreductase
RA 3,641,005 G→A S48S (TCG→TCA dtpB → dipeptide and tripeptide permease B
RA 3,655,517 A→G V129A (GTG→GCG)  yhiD ← putative Mg(2+) transport ATPase, inner membrane protein
RA 3,676,463 T→C T630A (ACG→GCG)  yhjG ← putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,703,956 A→G V269A (GTG→GCG)  dppC ← dipeptide/heme ABC transporter permease
RA 3,714,446 T→C Q650R (CAG→CGG)  bisC ← biotin sulfoxide reductase
RA 3,724,462 G→A A286V (GCA→GTA)  glyQ ← glycine tRNA synthetase, alpha subunit
RA 3,727,916 T→C intergenic (‑168/+1) yiaB ← / ← xylB YiaAB family inner membrane protein/xylulokinase
RA 3,728,118 (C)8→7 coding (1254/1455 nt) xylB ← xylulokinase
RA 3,789,720 T→C N121S (AAC→AGC)  waaH ← LPS(HepIII)‑glucuronic acid glycosyltransferase
RA 3,815,808 (A)8→7 intergenic (‑40/+26) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,832,490 T→C H683R (CAC→CGC)  yicI ← putative alpha‑glucosidase
RA 3,885,467 A→G Y131C (TAT→TGT)  yidC → membrane protein insertase
RA 3,903,442 G→A A87V (GCG→GTG)  bglB ← cryptic phospho‑beta‑glucosidase B
RA 3,932,390 C→T H359Y (CAC→TAC)  kup → potassium transporter
RA 3,939,197 T→C L324P (CTG→CCG)  rbsR → transcriptional repressor of ribose metabolism
RA 3,987,439 G→A A148A (GCC→GCT hemY ← putative protoheme IX synthesis protein
RA 4,128,247 C→T R50H (CGT→CAT)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding
RA 4,181,472 C→T G76G (GGC→GGT rpoB → RNA polymerase, beta subunit
RA 4,200,185 (A)7→6 intergenic (+91/‑96) yjaG → / → hupA DUF416 domain protein/HU, DNA‑binding transcriptional regulator, alpha subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,360,430 A→G V502A (GTA→GCA)  cadC ← cadBA operon transcriptional activator
RA 4,364,174 A→G V290A (GTG→GCG)  dsbD ← thiol:disulfide interchange protein and activator of DsbC
RA 4,388,960 C→T S137S (TCG→TCA mscM ← mechanosensitive channel protein, miniconductance
RA 4,399,151 Δ1 bp coding (1740/1848 nt) mutL → methyl‑directed mismatch repair protein
RA 4,442,003 A→G R58R (CGT→CGC msrA ← methionine sulfoxide reductase A
RA 4,573,096 A→G pseudogene (683/1503 nt) yjiT → pseudogene
RA 4,575,931 A→G pseudogene (2013/2937 nt) yjiV → pseudogene; conserved hypothetical protein
RA 4,582,590 A→G R153R (CGT→CGC hsdM ← DNA methyltransferase M
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423726–3424234 3424572–3424238 5–847 71 [68] [69] 70 [rrfD]–[rrlD] [rrfD], [rrlD]
* * ÷ NC_000913 3943470–3943987 3945515–3944061 75–2046 70 [67] [68] 72 [gltU]–[rrlC] [gltU], [rrlC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =15 (0.130)70 (0.660) 59/266 1.8 82.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 17 (0.160)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780516 (0.130)95 (0.900) 80/266 0.3 85.9% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 17 (0.160)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)91 (0.790) 81/290 0.6 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein