breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 1020 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913366,5260TG46.4% 160.8 / 51.4 114T329P (ACC→CCC) lacIlactose‑inducible lac operon transcriptional repressor
*NC_000913357,8900CA45.8% 46.2 / 33.9 60L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_0009132,571,0360TG45.5% 193.1 / 13.0 112N239T (AAC→ACC) eutEaldehyde oxidoreductase, ethanolamine utilization protein
*NC_00091329,4960TA44.6% 144.3 / 68.4 113intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
*NC_000913354,2880TG44.0% 200.4 / 34.3 116V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NC_0009133,206,4350CT43.8% 73.9 / 69.0 89intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
*NC_0009133,261,0550AC43.6% 199.4 / 22.5 110V455G (GTG→GGG) tdcEpyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
*NC_00091329,4860CA43.2% 194.9 / 82.0 111intergenic (+291/‑165)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
*NC_0009133,177,7480CA42.7% 176.9 / 72.1 118E55* (GAG→TAG) nudFADP‑ribose pyrophosphatase
*NC_0009133,350,8530AC42.2% 204.4 / 16.0 102S725A (TCA→GCA) arcBaerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
*NC_0009133,443,2770AC42.1% 216.2 / 31.2 114V274G (GTA→GGA) secYpreprotein translocase membrane subunit
*NC_0009131,097,5810AG41.9% 139.6 / 31.7 87noncoding (72/88 nt)serXtRNA‑Ser
*NC_0009131,376,5050AC41.8% 184.1 / 21.2 98T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
*NC_0009131,153,5730TG41.3% 116.1 / 42.5 75Y92D (TAC→GAC) pabC4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
*NC_000913573,4090CA41.3% 166.7 / 39.3 93S13R (AGC→AGArusADLP12 prophage; endonuclease RUS
*NC_0009134,604,3440CT41.2% 107.9 / 38.8 85S62F (TCT→TTT) bglJbgl operon transcriptional activator
*NC_0009135,1590GA41.1% 127.0 / 62.6 108intergenic (+139/‑75)thrC/yaaXL‑threonine synthase/DUF2502 family putative periplasmic protein
*NC_000913213,3410AG40.9% 189.5 / 40.0 116G337G (GGA→GGGtilStRNA(Ile)‑lysidine synthetase
*NC_0009133,492,8340TG40.7% 163.1 / 16.6 93G89G (GGT→GGGtsgAputative transporter
*NC_000913514,6240AC40.6% 174.2 / 11.6 96V79G (GTG→GGG) ybbJinner membrane protein; stimulator of the QmcA suppressor of ftsH‑htpX

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 925901 =107 (0.900)9 (0.080)
+C
7/296 19.8 8.7% noncoding (71/88 nt) serW tRNA‑Ser
?NC_000913 = 1097399 84 (0.710)intergenic (+570/+166) ycdU/serX putative inner membrane protein/tRNA‑Ser