breseq version 0.29.0 revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 1020 shown) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 366,526 | 0 | T | G | 46.4% | 160.8 / 51.4 | 114 | T329P (ACC→CCC) | lacI | lactose‑inducible lac operon transcriptional repressor |
* | NC_000913 | 357,890 | 0 | C | A | 45.8% | 46.2 / 33.9 | 60 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
* | NC_000913 | 2,571,036 | 0 | T | G | 45.5% | 193.1 / 13.0 | 112 | N239T (AAC→ACC) | eutE | aldehyde oxidoreductase, ethanolamine utilization protein |
* | NC_000913 | 29,496 | 0 | T | A | 44.6% | 144.3 / 68.4 | 113 | intergenic (+301/‑155) | dapB/carA | dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
* | NC_000913 | 354,288 | 0 | T | G | 44.0% | 200.4 / 34.3 | 116 | V528G (GTG→GGG) | prpE | propionate‑‑CoA ligase |
* | NC_000913 | 3,206,435 | 0 | C | T | 43.8% | 73.9 / 69.0 | 89 | intergenic (‑179/‑28) | ttdR/ttdA | transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit |
* | NC_000913 | 3,261,055 | 0 | A | C | 43.6% | 199.4 / 22.5 | 110 | V455G (GTG→GGG) | tdcE | pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase |
* | NC_000913 | 29,486 | 0 | C | A | 43.2% | 194.9 / 82.0 | 111 | intergenic (+291/‑165) | dapB/carA | dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
* | NC_000913 | 3,177,748 | 0 | C | A | 42.7% | 176.9 / 72.1 | 118 | E55* (GAG→TAG) | nudF | ADP‑ribose pyrophosphatase |
* | NC_000913 | 3,350,853 | 0 | A | C | 42.2% | 204.4 / 16.0 | 102 | S725A (TCA→GCA) | arcB | aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB |
* | NC_000913 | 3,443,277 | 0 | A | C | 42.1% | 216.2 / 31.2 | 114 | V274G (GTA→GGA) | secY | preprotein translocase membrane subunit |
* | NC_000913 | 1,097,581 | 0 | A | G | 41.9% | 139.6 / 31.7 | 87 | noncoding (72/88 nt) | serX | tRNA‑Ser |
* | NC_000913 | 1,376,505 | 0 | A | C | 41.8% | 184.1 / 21.2 | 98 | T158P (ACC→CCC) | ycjR | putative TIM alpha/beta barrel enzyme |
* | NC_000913 | 1,153,573 | 0 | T | G | 41.3% | 116.1 / 42.5 | 75 | Y92D (TAC→GAC) | pabC | 4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex |
* | NC_000913 | 573,409 | 0 | C | A | 41.3% | 166.7 / 39.3 | 93 | S13R (AGC→AGA) | rusA | DLP12 prophage; endonuclease RUS |
* | NC_000913 | 4,604,344 | 0 | C | T | 41.2% | 107.9 / 38.8 | 85 | S62F (TCT→TTT) | bglJ | bgl operon transcriptional activator |
* | NC_000913 | 5,159 | 0 | G | A | 41.1% | 127.0 / 62.6 | 108 | intergenic (+139/‑75) | thrC/yaaX | L‑threonine synthase/DUF2502 family putative periplasmic protein |
* | NC_000913 | 213,341 | 0 | A | G | 40.9% | 189.5 / 40.0 | 116 | G337G (GGA→GGG) | tilS | tRNA(Ile)‑lysidine synthetase |
* | NC_000913 | 3,492,834 | 0 | T | G | 40.7% | 163.1 / 16.6 | 93 | G89G (GGT→GGG) | tsgA | putative transporter |
* | NC_000913 | 514,624 | 0 | A | C | 40.6% | 174.2 / 11.6 | 96 | V79G (GTG→GGG) | ybbJ | inner membrane protein; stimulator of the QmcA suppressor of ftsH‑htpX |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 925901 = | 107 (0.900) | 9 (0.080) +C |
7/296 | 19.8 | 8.7% | noncoding (71/88 nt) | serW | tRNA‑Ser |
? | NC_000913 | = 1097399 | 84 (0.710) | intergenic (+570/+166) | ycdU/serX | putative inner membrane protein/tRNA‑Ser |