Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 160 117.2 4331776 85.4% 3699336 149.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,925,601 A→G R54R (CGA→CGG)  yobB → C‑N hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,925,6010AG97.7% 293.1 / ‑3.3 86R54R (CGA→CGG) yobBC‑N hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (41/43);  total (43/43)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGA  >  NC_000913/1925454‑1925748
                                                                                                                                                   |                                                                                                                                                   
aGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGt                                                                                                                                                  <  4:878980/151‑1 (MQ=255)
 gTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTg                                                                                                                                                 >  4:977611/1‑151 (MQ=255)
    gcagcaGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGca                                                                                                                                               <  2:444710/150‑2 (MQ=255)
      agcagcGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCtt                                                                                                                                            <  4:805024/151‑1 (MQ=255)
           cgcAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGc                                                                                                                                       >  3:452905/1‑151 (MQ=255)
            gcAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGcc                                                                                                                                      >  3:344579/1‑151 (MQ=255)
             cAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGccc                                                                                                                                     <  1:191550/151‑1 (MQ=255)
               atatGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCa                                                                                                                                   <  2:105468/151‑1 (MQ=255)
                    aGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCg                                                                                                                               >  4:1341281/1‑150 (MQ=255)
                          gTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGAtctctc                                                                                                                        >  4:696611/1‑151 (MQ=255)
                             aaaCCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACt                                                                                                                     <  4:1491729/151‑1 (MQ=255)
                              aaCCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTg                                                                                                                    >  4:484073/1‑151 (MQ=255)
                                 ctcactcaCCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGtt                                                                                                                  >  3:258670/1‑150 (MQ=255)
                                  tcactcacCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGtt                                                                                                                  >  1:227841/1‑149 (MQ=255)
                                   cactcacCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGtt                                                                                                                  >  3:107710/1‑148 (MQ=255)
                                   cactcacCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTa                                                                                                                 <  1:345316/149‑1 (MQ=255)
                                      tcacCGAACAGGCGGGTCATCATCTGGAGGTTGTGAGGGGCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGCTCTCTCACTGTTAGAccc                                                                                                            >  1:247524/1‑151 (MQ=255)
                                       cacCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGAccc                                                                                                            <  4:1679929/150‑1 (MQ=255)
                                       cacCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGAccc                                                                                                            <  1:452885/150‑1 (MQ=255)
                                       cacCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCg                                                                                                           >  2:946700/1‑151 (MQ=255)
                                       cacCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCg                                                                                                           <  4:1038422/151‑1 (MQ=255)
                                        acCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGc                                                                                                          >  4:575766/1‑151 (MQ=255)
                                         ccGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCt                                                                                                         >  2:52305/1‑151 (MQ=255)
                                                  tAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGc                                                                                                >  2:816071/1‑151 (MQ=255)
                                                   aGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGcc                                                                                               <  4:1019034/151‑1 (MQ=255)
                                                    gCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCg                                                                                              <  4:286149/151‑1 (MQ=255)
                                                    gCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCg                                                                                              >  4:868445/1‑151 (MQ=255)
                                                          catcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACg                                                                                         <  1:341507/150‑1 (MQ=255)
                                                          catcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGa                                                                                        <  4:1222321/151‑1 (MQ=255)
                                                            tcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGAcc                                                                                      >  1:268420/1‑151 (MQ=255)
                                                             cTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCt                                                                                     >  3:276341/1‑151 (MQ=255)
                                                               ggAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTg                                                                                    <  2:616642/150‑1 (MQ=255)
                                                                gAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGc                                                                                  <  4:785290/151‑1 (MQ=255)
                                                                 aGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCg                                                                                 >  4:869156/1‑151 (MQ=255)
                                                                    ttGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAAt                                                                              >  1:23835/1‑151 (MQ=255)
                                                                     tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAAt                                                                              <  4:797225/150‑1 (MQ=255)
                                                                      gtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATg                                                                             <  2:1442756/150‑1 (MQ=255)
                                                                        gAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGAc                                                                           <  4:771051/150‑1 (MQ=255)
                                                                            gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACcatca                                                                      <  2:596722/151‑1 (MQ=255)
                                                                             ccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACcatcat                                                                     >  2:448970/1‑151 (MQ=255)
                                                                              cgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCAtt                                                                    <  4:1384350/151‑1 (MQ=255)
                                                                              cgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCAtt                                                                    >  2:450724/1‑151 (MQ=255)
                                                                               gccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCAATg                                                                   >  3:165860/1‑151 (MQ=255)
                                                                                ccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGc                                                                  >  4:575466/1‑151 (MQ=255)
                                                                                    cAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGc                                                              <  2:1586319/151‑1 (MQ=255)
                                                                                    cAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGc                                                              >  2:440222/1‑151 (MQ=255)
                                                                                     aGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGcc                                                             >  4:952590/1‑151 (MQ=255)
                                                                                      ggCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCt                                                            >  4:452589/1‑151 (MQ=255)
                                                                                        caacaaTGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTc                                                          >  4:832980/1‑151 (MQ=255)
                                                                                           caaTGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCg                                                       <  1:107696/151‑1 (MQ=255)
                                                                                               gTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTg                                                    <  4:1117990/150‑1 (MQ=255)
                                                                                               gTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGa                                                   <  4:1472235/151‑1 (MQ=255)
                                                                                                tCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGa                                                   >  3:381347/1‑150 (MQ=255)
                                                                                                 cAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGaa                                                  <  4:368573/150‑1 (MQ=255)
                                                                                                 cAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAat                                                 <  3:9339/151‑1 (MQ=255)
                                                                                                 cAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAat                                                 <  4:1000858/151‑1 (MQ=255)
                                                                                                    cTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAatat                                               >  3:149166/1‑150 (MQ=255)
                                                                                                     tGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAAtata                                              >  4:1424378/1‑150 (MQ=255)
                                                                                                       ttAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACg                                           >  1:232334/1‑151 (MQ=255)
                                                                                                        tAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACg                                           <  2:516161/150‑1 (MQ=255)
                                                                                                               tCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCtt                                    <  4:567117/150‑1 (MQ=255)
                                                                                                                 cATCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTAt                                 <  2:1339604/151‑1 (MQ=255)
                                                                                                                  aTCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTAtt                                <  2:1403975/151‑1 (MQ=255)
                                                                                                                  aTCGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTAtt                                >  4:716961/1‑151 (MQ=255)
                                                                                                                    cGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCg                              >  1:88412/1‑151 (MQ=255)
                                                                                                                    cGCTTTCTTTACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCg                              >  3:200549/1‑151 (MQ=255)
                                                                                                                       tttctttACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTgg                           >  4:1523533/1‑151 (MQ=255)
                                                                                                                        ttctttACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCTCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTgg                           <  4:131992/150‑1 (MQ=255)
                                                                                                                        ttctttACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTg                            <  3:176018/149‑1 (MQ=255)
                                                                                                                           tttACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAAtt                       >  2:1374344/1‑151 (MQ=255)
                                                                                                                            ttACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGaa                         <  2:613408/148‑1 (MQ=255)
                                                                                                                             tACTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGc                     >  1:340719/1‑151 (MQ=255)
                                                                                                                              aCTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGc                     <  3:165358/150‑1 (MQ=255)
                                                                                                                              aCTGGGGTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCg                    <  2:976338/151‑1 (MQ=255)
                                                                                                                                tGGGGAGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGc                     >  2:1266711/1‑148 (MQ=255)
                                                                                                                                 ggggTGTGATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGgt                  >  4:1687495/1‑150 (MQ=255)
                                                                                                                                      gtgATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGc            <  1:258655/151‑1 (MQ=255)
                                                                                                                                        gATTATTCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCAc          <  4:774889/151‑1 (MQ=255)
                                                                                                                                          ttattCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCAcc         <  4:947933/150‑1 (MQ=255)
                                                                                                                                            attCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTg      <  2:729757/151‑1 (MQ=255)
                                                                                                                                            attCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTg      >  1:274123/1‑151 (MQ=255)
                                                                                                                                            attCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTg      >  4:1411896/1‑151 (MQ=255)
                                                                                                                                             ttCGCGGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTgg     >  4:480971/1‑151 (MQ=255)
                                                                                                                                               cgcgGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCTGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGc    <  2:932626/150‑1 (MQ=255)
                                                                                                                                               cgcgGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCg   <  4:62219/151‑1 (MQ=255)
                                                                                                                                               cgcgGCGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCg   >  1:213055/1‑151 (MQ=255)
                                                                                                                                                gcggcgTGCCCTTCCTGCCCCTCCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGa  <  2:374254/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGGAATTGCGGTGTTCGCACCGTGGCGA  >  NC_000913/1925454‑1925748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A