Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,226,817	A→G	Y73C (TAC→TGC)	ycgJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,226,817	0	A	G	100.0%	144.8 / NA	39	Y73C (TAC→TGC)	ycgJ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/21); total (18/21)

GACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCG  >  NC_000913/1226675‑1226961

gACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATcc                                                                                                                                            >  1:140327/1‑149 (MQ=255)

        cGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAAttt                                                                                                                                  <  1:237751/151‑1 (MQ=255)

                 aTCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCt                                                                                                                         >  2:183782/1‑151 (MQ=255)

                     ccGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACg                                                                                                                     <  1:556977/151‑1 (MQ=255)

                                  ttgttTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCg                                                                                                         >  2:161595/1‑150 (MQ=255)

                                      ttGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGtt                                                                                                    >  1:38644/1‑151 (MQ=255)

                                          gACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACAtt                                                                                                                             >  2:397076/1‑122 (MQ=255)

                                          gACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACAtt                                                                                                                             <  1:397072/122‑1 (MQ=255)

                                             ccGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGaaa                                                                                             <  2:488935/151‑1 (MQ=255)

                                                tataTTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTATATTTTGCGATGTTAAAGAAAAAt                                                                                          >  1:194538/1‑151 (MQ=255)

                                                         gtgAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtg                                                                                 <  1:86874/151‑1 (MQ=255)

                                                           gAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACACTCGCTAACGGTGTATTTTGCGATGTTAAACAAAAATTATGTCGtgat                                                                               <  1:222357/151‑1 (MQ=255)

                                                            aaCTCAGATGGCATTTCCCCAGAGTTAATAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtgatg                                                                              <  2:447318/151‑1 (MQ=255)

                                                             aCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTAtttt                                                                                                            <  1:156935/120‑1 (MQ=255)

                                                             aCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTAtttt                                                                                                            >  2:156938/1‑120 (MQ=255)

                                                             aCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtgatga                                                                             <  2:399290/151‑1 (MQ=255)

                                                              cTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTgatgat                                                                            >  2:247873/1‑151 (MQ=255)

                                                                 aGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGt                                                                         >  1:42306/1‑151 (MQ=255)

                                                                  gATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGtt                                                                        <  2:276541/151‑1 (MQ=255)

                                                                      gCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTAttt                                                                    <  2:223569/151‑1 (MQ=255)

                                                                        aTTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTg                                                                  <  2:103866/151‑1 (MQ=255)

                                                                        aTTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTg                                                                  >  2:132014/1‑151 (MQ=255)

                                                                           tCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGgt                                                                >  2:469676/1‑150 (MQ=255)

                                                                            ccccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGgtgt                                                              <  2:620637/151‑1 (MQ=255)

                                                                             cccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGtgtg                                                             >  2:430809/1‑151 (MQ=255)

                                                                                  agTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATgg                                                        <  2:650184/151‑1 (MQ=255)

                                                                                        cAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCg                                                  >  1:342794/1‑151 (MQ=255)

                                                                                        cAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCg                                                  >  1:328255/1‑151 (MQ=255)

                                                                                         aaGGAAATATCTCGGCGAAAAAGCCGCTGAAATCTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGGTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCg                                                  <  1:382270/150‑1 (MQ=255)

                                                                                            gAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGt                                              <  2:617619/151‑1 (MQ=255)

                                                                                                  tctcGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaaa                                         >  2:538553/1‑150 (MQ=255)

                                                                                                      ggtgAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaatca                                    <  2:338085/148‑1 (MQ=255)

                                                                                                                     gAAAACTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACcaca                      >  1:422582/1‑150 (MQ=255)

                                                                                                                        aaCTTACAATCATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACaacg                   >  1:473452/1‑148 (MQ=255)

                                                                                                                                  cATTACAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAATCACCACAAAGATGttatttat        <  1:262850/151‑1 (MQ=255)

                                                                                                                                      aCAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATgtgt    >  1:237675/1‑151 (MQ=255)

                                                                                                                                       cAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTc   <  1:433274/151‑1 (MQ=255)

                                                                                                                                       cAAGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTc   <  2:623692/151‑1 (MQ=255)

                                                                                                                                        aaGGCTGCGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCg  <  1:316007/151‑1 (MQ=255)

GACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCG  >  NC_000913/1226675‑1226961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A