breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 10,463 G→A P11L (CCG→CTG)  satP ← succinate‑acetate transporter
RA 17,348 A→G intergenic (‑388/‑141) mokC ← / → nhaA regulatory protein for HokC, overlaps CDS of hokC/sodium‑proton antiporter
RA 113,104 A→G V39A (GTG→GCG)  coaE ← dephospho‑CoA kinase
RA 121,578 (T)7→6 intergenic (‑27/‑514) aroP ← / → pdhR aromatic amino acid transporter/pyruvate dehydrogenase complex repressor; autorepressor
RA 125,921 C→T S76F (TCC→TTC)  aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 198,049 C→T A41V (GCG→GTG)  bamA → BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
RA 218,942 A→G V218A (GTT→GCT)  tsaA ← tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
RA 240,865 G→A A813A (GCC→GCT fadE ← acyl coenzyme A dehydrogenase
MC JC 257,908 Δ776 bp [crl] [crl]
RA 264,465 C→A E90* (GAG→TAG)  ykfG ← CP4‑6 prophage; RadC‑like JAB domain protein
RA 282,993 C→T A239V (GCC→GTC)  yagE → 2‑keto‑3‑deoxy gluconate (KDG) aldolase; CP4‑6 prophage
RA 287,616 G→A L276L (CTG→CTA yagH → CP4‑6 prophage; putative xylosidase/arabinosidase
RA 297,699 A→G pseudogene (21/339 nt) yagP ← pseudogene, LysR family, fragment;putative regulator; Not classified; putative transcriptional regulator LYSR‑type
RA 308,054 (C)7→8 coding (1279/2526 nt) ecpC ← ECP production outer membrane protein
RA 310,993 A→G V115A (GTA→GCA)  ecpR ← putative transcriptional regulator for the ecp operon
RA 329,853 A→G T131A (ACG→GCG)  betT → choline transporter of high affinity
RA 335,321 A→G A14A (GCA→GCG yahD → ankyrin repeat protein
RA 339,992 Δ1 bp intergenic (+249/‑173) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 340,028 T→C intergenic (+285/‑137) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 340,034 T→C intergenic (+291/‑131) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 340,051 A→G intergenic (+308/‑114) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 349,456 A→G T259A (ACG→GCG)  prpB → 2‑methylisocitrate lyase
RA 414,867 C→T W296* (TGG→TAG)  sbcC ← exonuclease, dsDNA, ATP‑dependent
RA 419,364 T→C intergenic (+180/‑227) phoR → / → brnQ sensory histidine kinase in two‑component regulatory system with PhoB/branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val
RA 468,271 (T)8→7 intergenic (+41/‑112) cof → / → ybaO thiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase/putative DNA‑binding transcriptional regulator
RA 481,469 A→G S979P (TCG→CCG)  acrB ← multidrug efflux system protein
RA 521,921 A→G V502V (GTA→GTG ybbP → putative ABC transporter permease
RA 576,168 T→C pseudogene (658/1040 nt) nmpC ← DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage
RA 577,085 G→A intergenic (‑260/‑313) nmpC ← / → essD DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage/DLP12 prophage; putative phage lysis protein
RA 588,485 G→A R824C (CGC→TGC)  nfrA ← bacteriophage N4 receptor, outer membrane subunit
RA 650,431 C→T V20V (GTG→GTA citE ← citrate lyase, citryl‑ACP lyase (beta) subunit
RA 693,599 T→C T453A (ACA→GCA)  miaB ← tRNA‑i(6)A37 methylthiotransferase
RA 717,098 A→G F390L (TTT→CTT)  potE ← putrescine/proton symporter: putrescine/ornithine antiporter
RA 741,429 A→G S376S (TCT→TCC dtpD ← dipeptide and tripeptide permease D
RA 781,084 T→C noncoding (17/76 nt) valZ → tRNA‑Val
RA 807,115 T→C Q56R (CAA→CGA)  ybhC ← acyl‑CoA thioesterase, lipoprotein
RA 835,945 A→G Y233C (TAT→TGT)  ybiB → putative family 3 glycosyltransferase
RA 847,016 A→G V35A (GTA→GCA)  glnP ← glutamine transporter subunit
RA 850,338 A→G intergenic (‑241/‑112) rhtA ← / → ompX threonine and homoserine efflux system/outer membrane protein X
RA 870,807 C→T A466V (GCG→GTG)  gsiB → glutathione ABC transporter periplasmic binding protein
RA 913,520 Δ1 bp coding (295/1653 nt) hcp ← hybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases
RA 927,272 T→C D54G (GAC→GGC)  aat ← leucyl/phenylalanyl‑tRNA‑protein transferase
JC JC 944,912 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp intergenic (+16/+14) dmsC → / ← ycaC dimethyl sulfoxide reductase, anaerobic, subunit C/putative isochorismatase family hydrolase
RA 1,028,132 A→G T63A (ACG→GCG)  yccU → putative CoA‑binding protein
RA 1,032,299 T→C V54A (GTG→GCG)  hyaA → hydrogenase 1, small subunit
RA 1,055,492 T→A A229A (GCA→GCT torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,116,797 A→G intergenic (‑215/‑10) lpxL ← / → yceA lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase/putative rhodanese‑related sulfurtransferase
RA 1,124,503 T→C H275R (CAC→CGC)  mdtH ← multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
RA 1,129,249 T→C L471L (TTG→CTG)  murJ → putative lipid II flippase
RA 1,154,782 A→G E224G (GAA→GGA)  yceG → septation protein, ampicillin sensitivity
RA 1,203,225 C→T intergenic (+1/‑43) croE → / → ymfL e14 prophage; putative DNA‑binding transcriptional regulator/e14 prophage; putative DNA‑binding transcriptional regulator
RA 1,226,817 A→G Y73C (TAC→TGC)  ycgJ → uncharacterized protein
RA 1,234,629 A→G I30T (ATC→ACC)  nhaB ← sodium:proton antiporter
RA 1,241,779 A→G L98P (CTG→CCG)  cvrA ← putative cation/proton antiporter
RA 1,246,015 T→C A454A (GCA→GCG treA ← periplasmic trehalase
RA 1,288,215 A→G L137P (CTG→CCG)  purU ← formyltetrahydrofolate hydrolase
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,307,582 C→T C355Y (TGC→TAC)  clsA ← cardiolipin synthase 1
RA 1,314,744 T→C T161A (ACT→GCT)  yciE ← putative rubrerythrin/ferritin‑like metal‑binding protein
RA 1,360,045 T→C Q288Q (CAA→CAG puuA ← glutamate‑‑putrescine ligase
RA 1,363,578 A→G D279G (GAC→GGC)  puuC → gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase; succinate semialdehyde dehydrogenase
RA 1,370,155 A→G intergenic (+152/‑61) pspE → / → ycjM thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein
RA 1,419,456 T→C M1M (ATG→GTG) † ydaG ← Rac prophage; uncharacterized protein
RA 1,432,298 A→G T1084A (ACG→GCG)  stfR → Rac prophage; putative tail fiber protein
RA 1,454,185 G→A G87S (GGC→AGC)  paaA → ring 1,2‑phenylacetyl‑CoA epoxidase subunit
RA 1,483,905 T→C V282A (GTA→GCA)  hrpA → putative ATP‑dependent helicase
RA 1,539,343 T→C E1083G (GAA→GGA)  narZ ← nitrate reductase 2 (NRZ), alpha subunit
RA 1,541,602 T→C Y330C (TAC→TGC)  narZ ← nitrate reductase 2 (NRZ), alpha subunit
RA 1,594,132 (C)8→9 pseudogene (3856/3861 nt) yneO ← pseudogene, AidA homolog
RA 1,609,015 A→G V3A (GTT→GCT)  yneE ← bestrophin family putative inner membrane protein
RA 1,630,461 A→G N151N (AAT→AAC ydfI ← putative NAD‑dependent D‑mannonate oxidoreductase
RA 1,687,757 T→C K207K (AAA→AAG fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,730,559 G→A P491P (CCG→CCA lhr → putative ATP‑dependent helicase
RA 1,754,748 A→G intergenic (‑271/+184) ydhY ← / ← ydhZ putative 4Fe‑4S ferridoxin‑type protein; FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex/uncharacterized protein
RA 1,756,590 T→C V298A (GTT→GCT)  pykF → pyruvate kinase I
RA 1,792,078 T→C intergenic (‑58/+189) ydiV ← / ← nlpC anti‑FlhD4C2 factor, inactive EAL family phosphodiesterase/putative C40 clan peptidase lipoprotein
RA 1,832,946 T→C R173R (CGT→CGC xthA → exonuclease III
RA 1,861,807 T→C A56A (GCA→GCG yeaC ← DUF1315 family protein
RA 1,863,313 T→C A181A (GCT→GCC gapA → glyceraldehyde‑3‑phosphate dehydrogenase A
RA 1,936,375 A→G intergenic (‑61/‑277) zwf ← / → yebK glucose‑6‑phosphate 1‑dehydrogenase/putative DNA‑binding transcriptional regulator
RA 1,956,520 T→C N163S (AAC→AGC)  torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit
RA 1,967,290 C→T G50S (GGT→AGT)  cheY ← chemotaxis regulator transmitting signal to flagellar motor component
RA 1,974,562 T→C T255A (ACG→GCG)  cheA ← fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein
RA 1,977,549 T→C Y99C (TAC→TGC)  flhC ← flagellar class II regulon transcriptional activator, with FlhD
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,000,046 G→A R114C (CGC→TGC)  fliY ← cystine transporter subunit
RA 2,016,401 C→T G178G (GGC→GGT fliH → negative regulator of FliI ATPase activity
RA 2,028,056 T→C intergenic (‑39/+132) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein
RA 2,036,629 A→G A265A (GCA→GCG hchA → glyoxalase III and Hsp31 molecular chaperone
RA 2,082,691 T→C intergenic (‑144/‑65) dacD ← / → sbcB D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b/exodeoxyribonuclease I; exonuclease I
RA 2,110,993 T→C L9L (TTA→TTG rfbA ← glucose‑1‑phosphate thymidylyltransferase
RA 2,117,002 T→C T42T (ACA→ACG wcaL ← putative glycosyl transferase
RA 2,136,954 A→G V97A (GTC→GCC)  wza ← colanic acid export protein; outer membrane auxillary lipoprotein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,177,605 T→C G320G (GGA→GGG fbaB ← fructose‑bisphosphate aldolase class I
RA 2,183,426 G→A Q76* (CAG→TAG)  yegX ← putative family 25 glycosyl hydrolase
RA 2,224,908 C→T intergenic (‑38/+136) pbpG ← / ← yohC D‑alanyl‑D‑alanine endopeptidase/Yip1 family inner membrane protein
RA 2,241,409 T→C Q87R (CAG→CGG)  galS ← galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor
RA 2,252,698 A→G V333V (GTA→GTG yeiI → putative kinase
RA 2,258,247 A→G F41L (TTC→CTC)  psuG ← pseudouridine 5'‑phosphate glycosidase
RA 2,272,752 C→T P130L (CCC→CTC)  yejA → microcin C ABC transporter periplasmic binding protein
RA 2,389,752 A→G N213D (AAT→GAT)  yfbP → TPR‑like repeats‑containing protein
RA 2,460,130 T→C M114V (ATG→GTG)  fadI ← beta‑ketoacyl‑CoA thiolase, anaerobic, subunit
RA 2,462,119 T→C F272L (TTT→CTT)  fadL → long‑chain fatty acid outer membrane transporter
RA 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein
RA 2,467,446 A→G K301R (AAG→AGG)  intS → CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase
RA 2,522,472 T→C E2E (GAA→GAG xapR ← transcriptional activator of xapAB
RA 2,530,256 G→A A326A (GCC→GCT zipA ← FtsZ stabilizer
RA 2,533,049 C→T A214V (GCG→GTG)  cysK → cysteine synthase A, O‑acetylserine sulfhydrolase A subunit
RA 2,609,458 T→C R324R (CGT→CGC hyfG → hydrogenase 4, subunit
JC 2,636,790 (TTC)3→2 coding (565‑567/1473 nt) der ← GTPase; multicopy suppressor of ftsJ
RA 2,647,150 G→A A59V (GCT→GTT)  pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
RA 2,662,523 A→G intergenic (‑392/+60) iscR ← / ← trmJ isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
RA 2,684,690 G→A A273V (GCG→GTG)  glyA ← serine hydroxymethyltransferase
RA 2,698,706 C→A intergenic (‑156/‑53) pgpC ← / → yfhH phosphatidylglycerophosphatase C, membrane bound/putative DNA‑binding transcriptional regulator
RA 2,718,339 A→G W64R (TGG→CGG)  yfiF ← putative methyltransferase
RA 2,764,190 A→G F322L (TTC→CTC)  yfjL ← CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein
RA 2,767,140 T→C L53L (TTG→CTG)  rnlB → CP4‑57 prophage; uncharacterized protein
RA 2,780,330 G→A G799G (GGC→GGT ypjA ← adhesin‑like autotransporter
RA 2,891,465 T→C N145S (AAC→AGC)  cysJ ← sulfite reductase, alpha subunit, flavoprotein
RA 2,898,881 C→T A180T (GCA→ACA)  ygcU ← putative FAD‑linked oxidoreductase
RA 2,912,885 A→G I256T (ATC→ACC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,931,401 A→G Q116R (CAG→CGG)  ygdG → Ssb‑binding protein, misidentified as ExoIX
RA 2,933,617 Δ1 bp coding (72/648 nt) fucA ← L‑fuculose‑1‑phosphate aldolase
RA 3,007,381 T→C S374P (TCC→CCC)  ygeW → putative carbamoyltransferase
RA 3,048,315 C→T V243I (GTC→ATC)  gcvP ← glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
RA 3,065,614 T→C R271R (CGT→CGC scpC → propionyl‑CoA:succinate CoA transferase
RA 3,086,618 A→G intergenic (+216/‑88) yqgC → / → metK uncharacterized protein/S‑adenosylmethionine synthetase
RA 3,091,787 A→G G218G (GGA→GGG rsmE → 16S rRNA m(3)U1498 methyltransferase, SAM‑dependent
RA 3,093,493 A→G H186R (CAC→CGC)  yqgE → uncharacterized protein
RA 3,196,367 A→T D129E (GAT→GAA hldE ← heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase
RA 3,266,688 C→T A126A (GCG→GCA tdcA ← tdc operon transcriptional activator
RA 3,270,143 C→T intergenic (+541/+482) yhaC → / ← garK pentapetide repeats‑related protein/glycerate kinase I
RA 3,272,271 Δ1 bp coding (481/885 nt) garR ← tartronate semialdehyde reductase
RA 3,295,531 T→C R46R (CGT→CGC yraN → UPF0102 family protein
RA 3,311,285 T→C intergenic (‑117/+130) pnp ← / ← rpsO polynucleotide phosphorylase/polyadenylase/30S ribosomal subunit protein S15
RA 3,313,236 T→C intergenic (‑58/+106) rbfA ← / ← infB 30s ribosome binding factor/translation initiation factor IF‑2
RA 3,376,403 T→C D125G (GAC→GGC)  sspB ← ClpXP protease specificity enhancing factor
RA 3,377,215 A→G L69P (CTG→CCG)  sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
RA 3,440,116 T→C D305G (GAC→GGC)  rpoA ← RNA polymerase, alpha subunit
RA 3,449,100 T→C T24A (ACG→GCG)  rplP ← 50S ribosomal subunit protein L16
RA 3,511,488 A→G L286P (CTC→CCC)  yhfZ ← putative DNA‑binding transcriptional regulator
RA 3,515,565 A→G Y117H (TAC→CAC)  dam ← DNA adenine methyltransferase
RA 3,524,787 T→C A639A (GCT→GCC mrcA → penicillin‑binding protein 1a, murein transglycosylase and transpeptidase
RA 3,525,382 A→G T838A (ACG→GCG)  mrcA → penicillin‑binding protein 1a, murein transglycosylase and transpeptidase
RA 3,552,089 T→C T129A (ACT→GCT)  malP ← maltodextrin phosphorylase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,575,617 G→A A183T (GCA→ACA)  yhgN → UPF0056 family inner membrane protein
RA 3,601,276 G→A P271S (CCG→TCG)  ftsX ← putative ABC transporter permease
RA 3,612,540 T→C W226R (TGG→CGG)  yhhT → UPF0118 family putative transporter
RA 3,631,895 G→A S232S (AGC→AGT yhiJ ← DUF4049 family protein
RA 3,717,476 T→C V56A (GTA→GCA)  ghrB → glyoxylate/hydroxypyruvate reductase B
RA 3,725,236 A→G V28A (GTT→GCT)  glyQ ← glycine tRNA synthetase, alpha subunit
RA 3,753,413 A→G Y229Y (TAT→TAC yiaV ← signal‑anchored membrane fusion protein (MFP) component of efflux pump
RA 3,770,892 T→C D163G (GAC→GGC)  yibH ← putative membrane fusion protein (MFP) component of efflux pump
RA 3,781,563 G→A E117K (GAA→AAA)  trmL → tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,788,944 T→C F282L (TTT→CTT)  yibQ → putative polysaccharide deacetylase
RA 3,790,211 T→C intergenic (‑130/+109) waaH ← / ← tdh LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding
RA 3,815,808 (A)8→7 intergenic (‑40/+26) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,828,150 G→A G172G (GGC→GGT gltS ← glutamate transporter
RA 3,836,534 (C)6→7 pseudogene (110/132 nt) yicT → pseudogene
RA 3,893,403 C→T S213S (AGC→AGT yidZ → putative DNA‑binding transcriptional regulator
RA 3,907,945 T→C N141S (AAC→AGC)  pstB ← phosphate ABC transporter ATPase
RA 3,914,638 G→C T188S (ACC→AGC)  glmU ← fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase
RA 3,916,435 G→A A314V (GCG→GTG)  atpD ← F1 sector of membrane‑bound ATP synthase, beta subunit
RA 3,940,308 A→G L102P (CTG→CCG)  hsrA ← putative multidrug or homocysteine efflux system
RA 3,943,370 C→T intergenic (+21/‑65) rrsC → / → gltU 16S ribosomal RNA of rrnC operon/tRNA‑Glu
RA 3,949,005 T→C Q322R (CAG→CGG)  yifB ← magnesium chelatase family protein and putative transcriptional regulator
RA 3,951,452 C→T pseudogene (893/984 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
RA 3,967,093 A→G E226G (GAA→GGA)  rho → transcription termination factor
RA 4,003,303 A→G R292R (CGT→CGC rarD ← putative chloramphenical resistance permease
RA 4,017,296 T→C intergenic (+104/‑37) udp → / → rmuC uridine phosphorylase/DNA recombination protein
RA 4,098,989 A→G H115R (CAC→CGC)  rhaR → transcriptional activator of rhaSR
RA 4,102,930 C→T L37L (CTG→TTG)  yiiM → 6‑N‑hydroxylaminopurine resistance protein
RA 4,109,803 G→A D324N (GAT→AAT)  sbp → sulfate transporter subunit
RA 4,128,316 A→G V27A (GTT→GCT)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding
RA 4,129,743 A→G T358A (ACG→GCG)  metB → cystathionine gamma‑synthase, PLP‑dependent
RA 4,143,617 A→G intergenic (+8/‑7) frwC → / → frwB putative enzyme IIC component of PTS/putative enzyme IIB component of PTS
RA 4,154,549 T→C E100G (GAG→GGG)  argE ← acetylornithine deacetylase
RA 4,185,098 A→G Y1285C (TAC→TGC)  rpoB → RNA polymerase, beta subunit
RA 4,188,413 C→T P1022S (CCG→TCG)  rpoC → RNA polymerase, beta prime subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,307,043 C→T A253T (GCC→ACC)  alsK ← D‑allose kinase
RA 4,342,193 A→G T95A (ACG→GCG)  melA → alpha‑galactosidase, NAD(P)‑binding
RA 4,356,166 C→T A82A (GCG→GCA dtpC ← dipeptide and tripeptide permease
RA 4,357,602 A→G F339S (TTC→TCC)  cadA ← lysine decarboxylase, acid‑inducible
RA 4,366,223 A→G F184S (TTC→TCC)  dcuA ← C4‑dicarboxylate antiporter
RA 4,399,151 Δ1 bp coding (1740/1848 nt) mutL → methyl‑directed mismatch repair protein
RA 4,453,330 A→G N254D (AAC→GAC)  ytfT → putative sugar ABC transporter permease
RA 4,485,872 C→T R27H (CGC→CAC)  pepA ← multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
RA 4,503,643 T→C intergenic (‑212/‑415) insG ← / → yjhB IS4 transposase/putative MFS transporter, membrane protein
RA 4,550,206 G→A A364V (GCC→GTC)  gntP ← fructuronate transporter
RA 4,586,424 C→T R113H (CGC→CAC)  hsdR ← endonuclease R Type I restriction enzyme

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423736–3424233 3424613–3424238 6–878 16 [15] [15] 17 [rrfD]–[rrlD] [rrfD], [rrlD]
* * ÷ NC_000913 4035275–4035732 4037742–4035736 5–2468 16 [15] [15] 16 rrsA–[rrlA] rrsA, ileT, alaT, [rrlA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =3 (0.080)23 (0.650) 23/266 0.9 81.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 8 (0.230)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078053 (0.080)30 (0.850) 28/266 0.5 84.9% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 8 (0.230)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related