Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,609,015	A→G	V3A (GTT→GCT)	yneE ←	bestrophin family putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,609,015	0	A	G	100.0%	148.6 / NA	41	V3A (GTT→GCT)	yneE	bestrophin family putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/15); total (26/15)

GAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCAT  >  NC_000913/1608867‑1609158

gAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCa                                                                                                                                               <  2:420873/151‑1 (MQ=255)

                tGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTc                                                                                                                                >  1:636445/1‑150 (MQ=255)

                 gCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTc                                                                                                                                >  2:372264/1‑149 (MQ=255)

                    tgtACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCg                                                                                                                           >  2:222726/1‑151 (MQ=255)

                       aCCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTgg                                                                                                                        >  2:351857/1‑151 (MQ=255)

                         cAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGaa                                                                                                                      <  2:75700/151‑1 (MQ=255)

                           gggCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGaaaa                                                                                                                    <  2:273983/151‑1 (MQ=255)

                               agcaTGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCg                                                                                                                >  2:37550/1‑151 (MQ=255)

                                  aTGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGccc                                                                                                             >  2:553747/1‑151 (MQ=255)

                                      aaaTAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTAtt                                                                                                         >  1:492109/1‑151 (MQ=255)

                                            cAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGt                                                                                                   <  1:253287/151‑1 (MQ=255)

                                               cGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGAt                                                                                                >  1:654149/1‑151 (MQ=255)

                                               cGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGAt                                                                                                >  2:416221/1‑151 (MQ=255)

                                                  tAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTAc                                                                                             >  2:310959/1‑151 (MQ=255)

                                                   aGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACg                                                                                            <  1:568538/151‑1 (MQ=255)

                                                         gagaAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATcac                                                                                       >  2:665434/1‑150 (MQ=255)

                                                                   agtagtAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGAc                                                                            <  2:558787/151‑1 (MQ=255)

                                                                    gtagtaAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACg                                                                           <  2:112671/151‑1 (MQ=255)

                                                                          aGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACtt                                                                     >  1:474153/1‑151 (MQ=255)

                                                                          aGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACtt                                                                     >  1:350039/1‑151 (MQ=255)

                                                                                 ggATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAAtt                                                              <  1:35417/151‑1 (MQ=255)

                                                                                  gATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTa                                                             <  2:654160/151‑1 (MQ=255)

                                                                                     atCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCt                                                          >  1:221516/1‑151 (MQ=255)

                                                                                         tGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACa                                                       <  1:15161/150‑1 (MQ=255)

                                                                                            ataataCTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCt                                                   <  2:35064/151‑1 (MQ=255)

                                                                                            ataataCTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCt                                                   >  2:506564/1‑151 (MQ=255)

                                                                                                  cTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTacaac                                             >  1:6789/1‑151 (MQ=255)

                                                                                                     aGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACAtt                                          >  1:295162/1‑151 (MQ=255)

                                                                                                     aGCCGTGCCAGACAAAAATACGGCGCAGACAGTGTTGCTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGAAAAAGTCACCCTATTAAACGTGATTACGATCACATTCTCAAGACCCTAATTACAAATTACCTACAAGCTTACAACAtt                                          >  1:519032/1‑151 (MQ=255)

                                                                                                           gCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAgg                                    >  2:28983/1‑151 (MQ=255)

                                                                                                                aCAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTg                               >  2:179450/1‑151 (MQ=255)

                                                                                                                  aaaaaTACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGc                              >  1:429230/1‑150 (MQ=255)

                                                                                                                     aaTACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCAtgt                          <  2:492111/151‑1 (MQ=255)

                                                                                                                     aaTACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCAtgt                          >  2:200575/1‑151 (MQ=255)

                                                                                                                     aaTACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCAtgt                          <  2:577728/151‑1 (MQ=255)

                                                                                                                       tACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCtt                                                  <  1:251210/125‑1 (MQ=255)

                                                                                                                       tACGGCGCAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCtt                                                  >  2:251211/1‑125 (MQ=255)

                                                                                                                             gcAGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTg                  <  2:295164/151‑1 (MQ=255)

                                                                                                                               atccAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGtt                >  2:120314/3‑151 (MQ=255)

                                                                                                                               aGCCAGTGTTGTTGTGGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGtt                >  2:183174/1‑151 (MQ=255)

                                                                                                                                             tgGACGAGCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCat  >  1:630472/1‑151 (MQ=255)

GAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCAT  >  NC_000913/1608867‑1609158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A